Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized KLRK1 (CSB-MP012474HU1) at 10 μg/ml can bind human Biotinylated ULBP1, the EC₅₀ is 4.254-7.295 ng/ml.

Target Names

ULBP1

Uniprot No.

Q9BZM6

Alternative Names

(ALCAN-beta)(NKG2D ligand 1)(N2DL-1)(NKG2DL1)(Retinoic acid early transcript 1I)(N2DL1)(RAET1I)

Molecular Characterization

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

26-216aa

Target Protein Sequence

GWVDTHCLCYDFIITPKSRPEPQWCEVQGLVDERPFLHYDCVNHKAKAFASLGKKVNVTKTWEEQTETLRDVVDFLKGQLLDIQVENLIPIEPLTLQARMSCEHEAHGHGRGSWQFLFNGQKFLLFDSNNRKWTALHPGAKKMTEKWEKNRDVTMFFQKISLGDCKMWLEEFLMYWEQMLDPTKPPSLAPG

Mol. Weight

51.3 kDa

Protein Length

Full Length of Mature Protein

Tag Info

C-terminal mFc-Avi-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant human ULBP1 protein is a biotinylated protein with relatively high bioactivity. It is produced in mammalian cells and fused with an mFc-Avi-tag at the C-terminus. Its expression region corresponds to amino acid residues 26-216 of the human ULBP1 protein. The purity of this ULBP1 protein is measured by SDS-PAGE and reaches up to 95%. On the gel, it has an apparent molecular mass of 67 kDa due to glycosylation. It contains low levels of endotoxin, less than 1.0 EU/ug determined by the LAL method. In the functional ELISA, this biotinylated ULBP1 can bind to the KLRK1, with EC₅₀ of 4.254-7.295 ng/ml. This biotinylated ULBP1 could be used to isolate ULBP1 antibodies from samples for subsequent analyses with high sensitivity. And it is available now.

ULBP1 is a stress-induced ligand for the activatory receptor NKG2D. It is found as a cell-surface protein on some malignant cells including human hepatocellular carcinomas. Upregulation of ULBP1 is detected during HCMV infection.

Target Background

Function

Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.

Gene References into Functions

we show that Simian Virus 40 (SV40)...evades NK cell attack through the down regulation of...ULBP1 PMID: 26992229
ATF4 drives ULBP1 gene expression in cancer cell lines, while the RNA-binding protein RBM4 supports ULBP1 expression by suppressing a novel alternatively spliced isoform of ULBP1 mRNA. PMID: 26565589
expression determines intrinsic acute myeloid leukemia susceptibility to allogeneic V[gamma]9V[delta]2 T cells PMID: 24911793
recurrence-free survival of patients with ULBP1-negative hepatocellular carcinoma (HCC) was significantly shorter than that of patients with ULBP1-positive HCC PMID: 21756848
recombinant ULBP1 fused to CD45 caused a reduction in cytotoxicity and degranulation by NK cells, implying a role for receptor ligand distribution in the activation of NK cell responses PMID: 21464092
Data show that ULBP1, TFR2 and IFITM1 were associated with increased susceptibility to Vgamma9Vdelta2 T-cell cytotoxicity. PMID: 20220060
These results identify Mult1 as a target for the MARCH family of E3 ligases PMID: 20870941
As NKG2D ligand, ULBP1 are expressed on immature dendritic cells and plays an important role in the cytotoxic effect of NK cells against iDC. PMID: 18394338
Data show that the protease NS3/4A of HCV down-regulates ULBP1 expression by inhibiting the transcription of ULBP1. PMID: 19500498
ULBP1 binds to the NKG2D receptor and activates multiple signaling pathways in primary natural killer cells. PMID: 11777960
The NKG2D ligand ULBP1 is up-regulated and readily detectable intracellularly in the endoplasmic reticulum of human cytomegalovirus-infected fibroblasts, where it colocalizes with viral protein UL16. PMID: 12847260
ULBP1 is a human ligand of the NKG2D receptor PMID: 16901903
The selective induction of ULBP1 expression by proteasome inhibitor drugs, along with variable NKG2D ligand expression by human tumor cells, indicates that NKG2D ligand genes are independently regulated. PMID: 19414815

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor. Endoplasmic reticulum.

Protein Families

MHC class I family

Tissue Specificity

Expressed in T-cells, B-cells, erythroleukemia cell lines and in a wide range of tissues including heart, brain, lung, liver, testis, lymph node, thymus, tonsil and bone marrow. Also found in fetal heart, brain, lung and liver.

Database Links

HGNC: 14893

OMIM: 605697

KEGG: hsa:80329

STRING: 9606.ENSP00000229708

UniGene: Hs.653255

Code	CSB-MP887177HUj1-B
Abbreviation	Recombinant Human ULBP1 protein, Biotinylated (Active)
MSDS	MSDS Datasheet
Size	$338
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized KLRK1 (CSB-MP012474HU1) at 10 μg/ml can bind human Biotinylated ULBP1, the EC₅₀ is 4.254-7.295 ng/ml. Biological Activity Assay
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Recombinant Human UL16-binding protein 1 (ULBP1),Biotinylated (Active)

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