OCLN Antibody

Code CSB-PA016263LA01HU
Size US$299
Image
  • Western Blot
    Positive WB detected in: MCF-7 whole cell lysate
    All lanes: OCLN antibody at 3.2μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 60, 53, 55, 32, 24, 9 kDa
    Observed band size: 60 kDa

  • IHC image of CSB-PA016263LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of MCF-7 cells with CSB-PA016263LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Description

CUSABIO is glad to provide a highly specific antibody directed against an epitope of human OCLN. The antibody (CSB-PA016263LA01HU) is produced in the rabbit against a peptide corresponding to amino acid residues 285-430 of human OCLN. It exists as an unconjugated IgG isotype. It is protein G purified and reaches up to 95% in purity. This OCLN antibody has been validated for use in ELISA, WB, IHC, and IF analyses. And it has been designed to recognize OCLN from human samples. Its target protein OCLN may maintain tight junction (TJ) barriers, signaling and junction remodeling, and mediate cytokine-induced changes in barrier properties.

Full Product Name Rabbit anti-Homo sapiens (Human) OCLN Polyclonal antibody
Uniprot No. Q16625
Target Names OCLN
Alternative Names BLCPMG antibody; FLJ08163 antibody; FLJ18079 antibody; FLJ77961 antibody; FLJ94056 antibody; MGC34277 antibody; Occludin antibody; Ocln antibody; OCLN_HUMAN antibody; Phosphatase 1 regulatory subunit 115 antibody; PPP1R115 antibody; PTORCH1 antibody; Tight junction protein occludin antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Occludin protein (285-430AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
Gene References into Functions
  1. our findings for the first time identify the role of occludin as a tumor promoter and a prometastatic factor in lung cancer, demonstrating that occludin is a potential prognostic biomarker and therapeutic target in lung cancer PMID: 29750300
  2. Occludin protein may contribute to the development of cervical cancer. However, it was not correlated with the clinical features. PMID: 29516973
  3. Low occludin expression is associated with Renal cell carcinoma. PMID: 28534944
  4. decreased interaction between ZO-1 and occludin might contribute to the epiphora occurred in the transplanted submandibular glands PMID: 28332063
  5. integration of claudin-2, occludin and ZO-1 is necessary for maintaining the function of the proximal tubular epithelium. PMID: 29252987
  6. Molecular studies of the OCLN gene (NM_001205254) identified six distinct candidate mutations in polymicrogyria. PMID: 28179633
  7. Glutamine increased claudin-1 expression in the colonic mucosa of patients with irritable bowel syndrome. In contrast, occludin expression was not significantly modified by glutamine. PMID: 25972430
  8. Endothelial cellsTLR4 strongly regulates retinal vessel permeability by reducing expression of occludin and zonula occludens 1. PMID: 29136627
  9. Suggest endothelial progenitor cell occluding regulates tube formation, sprouting, and proliferation. PMID: 28681912
  10. Viral infectivity was significantly reduced by miR-200c but enhanced by miR-122. PMID: 28642978
  11. The mechanism of transition from nondifferentiated to differentiated states in HepaRG cells was studed by proteomics. Two key factors (MMP-14 and OCLN) were validated by qRT-PCR and Western blot. PMID: 27790907
  12. Intracellular zinc has an essential role in the maintenance of the intestinal epithelial tight junction barrier through regulation of occludin proteolysis and claudin-3 transcription. PMID: 27151944
  13. Data suggest that long noncoding RNA PlncRNA1 and microRNA miR-34c bound together to regulate the expressions of MAZ, ZO-1 and occludin. PMID: 28153728
  14. Study provides evidence that occludin contributes to the regulation of size-reductive proliferation and epithelial cell maturation in a phosphorylation-dependent manner. PMID: 27185880
  15. Report shows that silencing MARCH3 protects the endothelial barrier and upregulates OCLN in MARCH3-depleted cells. MARCH3 silencing results in the strengthening of cell-cell contacts and inactivates FoxO1. PMID: 27616439
  16. STAT3 activation downregulates the ZO-1 and occludin levels and increases the endothelial permeability through the induction of VEGF production in retinal endothelial cells. PMID: 27580405
  17. Downregulation of OCLN is associated with clear cell renal cell carcinoma. PMID: 28184927
  18. These data suggest that the Rho/ROCK signaling pathway is involved in HIV-1 Tat-mediated changes in occludin, RAGE, and LRP1 in human cerebral microvascular endothelial cells cells. PMID: 27563375
  19. OCLN is essential for HCV infection of human hepatic cells. PMID: 26887345
  20. OCLN and ZO1 levels appear to be early prognostic markers in patients suffering from sepsis. PMID: 26863122
  21. autotypic tight junctions molecular composition, like claudin-1 and occludin expression could influence the demyelinating process by altering the permeability of the blood-nerve barrier. PMID: 26662145
  22. Occludin expression has a clear relationship with bone metastasis in human cancer. PMID: 26977027
  23. This is report of a family of Indian origin with two affected sibs and segregation of a homozygous novel OCLN mutation in the exon 3(NG_028291.1(OCLN_v001):c.252delC). PMID: 26689621
  24. Data show that estrogen mediates control of hepatitis C virus through the G-protein-coupled estrogen receptor 30 (GPR30) pathway leading to cleavage of occludin by Matrix Metalloproteinase-9 (MMP-9). PMID: 26731262
  25. In hepatitis C virus -infected human livers, occludin and ADRP mRNA expression levels correlated with each other. Hepatitis C virus increases occludin expression via the upregulation of ADRP. PMID: 26731658
  26. Data show that claudin-6 (CLDN6) R209Q and occludin (OCLN) P24A mutations do not affect HCV pseudoparticles (HCVpp) entry. PMID: 26561856
  27. resistance to HCV in HIV+ patients may be related to genetic variation in SCARB1 or OCLN PMID: 26571379
  28. Angiopoietin-1 Regulates Brain Endothelial Permeability through PTPN-2 Mediated Tyrosine Dephosphorylation of Occludin PMID: 26090670
  29. TIMP1 downregulates MMP-2 activity and inhibits the degradation of occluding in Caco-2 intestinal cells PMID: 25665057
  30. Study uncovers a novel antiviral effect of miR-122 on human liver cells and shows that over-expression of miR-122 can decrease HCV entry into hepatocytes through down-regulation of OCLN. PMID: 25302477
  31. Lipid raft-associated processes, such as PP2A and MMP-2 activation, participate in PCB153-induced disruption of occludin function in brain endothelial barrier. PMID: 26080028
  32. miR-18a and RUNX1 could reversely regulate the permeability of blood-tumor barrier as well as the expressions and distributions of ZO-1, occludin and claudin-5. PMID: 25452107
  33. Zonula occludens-1, occludin and E-cadherin expression and organization in salivary glands PMID: 25248927
  34. LL-37 selectively increased the expression of several claudins and occludin, and enhanced their membrane distribution. PMID: 24862212
  35. In conclusion, our present study indicated that miR-34c regulated the permeability of BTB via MAZ-mediated expression changes of ZO-1, occludin, and claudin-5. PMID: 25201524
  36. findings suggest that cerebral vascular cells express functional BACE1. Moreover, elevated vascular BACE1 may contribute to deficiency of occludin in caa. PMID: 24739782
  37. luciferase assays and chromatin immunoprecipitation assays showed that KLF4 up-regulated the promoter activities and interacted with "CACCC" DNA sequence presented in the promoters of ZO-1, occludin, and claudin-5. PMID: 24318462
  38. We conclude that OCEL-mediated occludin interactions are essential for limiting paracellular macromolecular flux. PMID: 23924897
  39. Data suggest components of diet supplements (here, glutamine/arginine) can improve permeability and tight junction protein expression (OCLN/zona occludens 1) in enterocytes exposed to deleterious effects of antineoplastic agents (here, methotrexate). PMID: 23428392
  40. Eosinophilic esophagitis (EoE), an inflammatory atopic disease of the esophagus, causes massive eosinophil infiltration, basal cell hyperplasia, and sub-epithelial fibrosis. Western analysis of pinch biopsies from EoE and normal pediatric patients indicated reduced expression of intercellular junction proteins, E-cadherin and claudin-1 and increased expression of occludin and vimentin. PMID: 23792687
  41. actin cytoskeletal dynamics is detrimental to METH-induced BBB dysfunction by increasing internalization of occludin. PMID: 24081143
  42. identification of a novel deletion/rearrangement of OCLN that results in band-like brain calcification, limited cognitive abilities, failure to thrive and renal dysfunction in early childhood PMID: 23793442
  43. In HepG2 cells, both hepatitis C virus cell entry and tight junction formation were impaired by OCLN silencing and restored by expression of antibody regulatable OCLN mutant. PMID: 23555257
  44. Loss of occludin is involved in adhesion, apoptosis, differentiation, Ca2+-homeostasis and neoplastic transformation in UV-exposed human keratinocytes and in skin neoplasms. PMID: 23390516
  45. Sinonasal epithelium in allergic fungal rhinosinusitis displays increased epithelial permeability and an altered expression of occludin. PMID: 22927233
  46. HuR promotes occludin translation by blocking occludin mRNA translocation to P-bodies via the displacement of CUGBP1. PMID: 23155001
  47. It is concluded that attenuated expression of the TJ proteins occludin and ZO-1 in human gastric epithelial cells could be involved in clopidogrel-induced gastric mucosal injury through activation of the p38 MAPK pathway PMID: 23220562
  48. Elevated occludin expression is associated with active ulcerative colitis. PMID: 22134947
  49. TTF-1 transcriptionally regulates occludin PMID: 22761434
  50. We define the interface between the ZO-1 PDZ3-SH3-U5-GuK (PSG) and occludin coiled-coil (CC) domains. PMID: 22711802

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Involvement in disease Pseudo-TORCH syndrome 1 (PTORCH1)
Subcellular Location Cell membrane, Multi-pass membrane protein, Cell junction, tight junction
Protein Families ELL/occludin family
Tissue Specificity Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Database Links

HGNC: 8104

OMIM: 251290

KEGG: hsa:100506658

STRING: 9606.ENSP00000347379

UniGene: Hs.592605

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