STAT5B Antibody

Code CSB-PA022815ESR1HU
Size US$167Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western blot
    All lanes: STAT5B antibody at 16 μg/ml
    Lane 1: HepG2 whole cell lysate
    Lane 2: K562 whole cell lysate
    Lane 3: Hela whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 90 kDa
    Observed band size: 90 kDa

  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA022815ESR1HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA022815ESR1HU at dilution of 1:100

  • Chromatin Immunoprecipitation MCF-7 (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-STAT5B or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers (CSB-PP022815HU) against the AGT promoter.

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) STAT5B Polyclonal antibody
Uniprot No. P51692
Target Names STAT5B
Alternative Names Signal transducer and activator of transcription 5B antibody; STA5B_HUMAN antibody; STAT5 antibody; Stat5b antibody; Transcription factor STAT5B antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Signal transducer and activator of transcription 5B protein (40-280AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form Liquid
Tested Applications ELISA, WB, IHC, ChIP
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Carries out a dual function
Gene References into Functions
  1. STAT5B germline mutation variants exert dominant-negative effects through distinct pathomechanisms, manifesting in milder clinical growth hormone insensitivity syndrome with general sparing of the immune system. PMID: 29844444
  2. Study investigated whether RGS4 could participate in signalling pathways to regulate neurotropic events. These observations suggest that RGS4 is implicated in opioid dependent neuronal differentiation and neurite outgrowth via a "non-canonical" signaling pathway regulating STAT5B-directed responses. PMID: 28219718
  3. Somatic STAT5B N642H gain-of-function mutation in early onset nonclonal eosinophilia, urticaria, dermatitis, and diarrhea in two young patients. PMID: 27956386
  4. High incidence of activating STAT5B mutations in CD4-positive T-cell large granular lymphocyte leukemia. PMID: 27697773
  5. STAT5 signaling drives antigen restimulation-induced T cell death in effector memory T cells PMID: 29187589
  6. Data show that BACH2 and STAT5B are activated by viral insertions, generating chimeric mRNAs specifically enriched in T regulatory cells favoring their persistence. PMID: 28887441
  7. The findings, specifically frequent mutations of STAT5B, PIK3CD, and the histone methyltransferase SETD2, may help guide translational efforts to target hepatosplenic T-cell lymphoma PMID: 28373165
  8. this study shows that musculin inhibits human T-helper 17 cell response to interleukin 2 by controlling STAT5B activity PMID: 28612433
  9. Molecular interactions of EphA4, growth hormone receptor, Jak2, and STAT5B have been described. PMID: 28686668
  10. All merkel cell carcinoma (MCC) cases with a good outcome expressed pSTAT5B, whereas all MCC cases with a poor outcome did not express pSTAT5B; hence, pSTAT5B expression may be an indicator of positive prognosis in patients with MCC. PMID: 28476799
  11. STAT5b confers gemcitabine chemoresistance and promotes cell adherence and invasiveness in pancreatic cancer cells. PMID: 27035235
  12. STAT5 activation by EGF constitutes an important cascade for the regulation of cell proliferation and invasion in trophoblast cells. PMID: 25862676
  13. MSM decreased the ability of STAT5b to bind the promoter of the HER2 gene. PMID: 26648017
  14. Results confirm that STAT5B is mutated in T-PLL and underscore the potential therapeutical relevance of epigenetic regulator. PMID: 26917488
  15. ABL-N administration induced apoptosis of PC3 cells in a dose-dependent manner, along with the enhanced activity of caspases and increased Bax/Bcl-2 ratio. Expression of KLF5, Stat5b and ICAM-1 was significantly downregulated in PC3 cells. PMID: 26397390
  16. Interference of STAT 5b expression by siRNA targeting enhanced the chemo-sensitivity of gastric cancer cells to gefitinib by promoting mitochondrial pathway-mediated cell apoptosis. PMID: 25997700
  17. Our study also assessed the regulation of signaling molecules implicated in the growth, progression, differentiation, and migration of cancer cells, such as Jak2, STAT5b, insulin-like growth factor-1Rbeta, and their phosphorylation status PMID: 26084564
  18. Statistically significant positive correlation has been found between STAT5B and COX-2, and significant negative correlation between STAT5B and PIAS3. PMID: 25137041
  19. STAT5B mutations in the heterozygous state have a significant negative impact on height ( approximately 3.9 cm). This effect is milder than the effect seen in the homozygous state, with height usually within the normal range. PMID: 26034074
  20. Expression of STAT5B was significantly correlated with astrocytoma tumor grade and Karnofsky Performance Scale score. High expressions of NPM, p-JAK2, and STAT5B were associated with a short survival time. PMID: 25907517
  21. study showed that a common mutation of STAT5B results in constitutive STAT5B phosphorylation, growth factor-independent proliferation in cell-based assays, activation of downstream targets in leukemia cells of children with T-cell acute lymphoblastic leukemia and a higher risk of relapse PMID: 24972766
  22. STAT5b rs2293157 G/T genotype was at increased risk of glioma. Furthermore, rs2293157T allele was more significantly prognostic in patients suffering from glioblastoma compared to other subtypes of gliomas PMID: 24878107
  23. Data indicate no STAT5 transcription factor STAT5B mutation was identified in 65 adult T-cell acute lymphoblastic leukemia (T-ALL) patients, and only one STAT5B N642H mutation was detected in 28 pediatric patients. PMID: 25749351
  24. Results identify miR-134 as a novel RTK-regulated tumor-suppressive hub that mediates RTK and RTK-inhibitor effects on GBM malignancy by controlling KRAS and STAT5B. PMID: 24440911
  25. We report a high frequency of STAT5B and STAT5 mutations in gamma-delta hepatosplenic T-cell lymphoma. PMID: 24947020
  26. Macrophage migration inhibitory factor induced accumulation of mast cells in vivo is associated with activation of STAT5. PMID: 24091309
  27. STAT5B homozygous mutation is responsible for a new growth hormone insensitivity syndrome associated to immunological dysfunction. PMID: 23896798
  28. Data indicate that cyclooxygenase-2 (COX-2) expression is dependent on STAT5 phosphorylation. PMID: 23601296
  29. our data suggest that the CD82/STAT5/IL-10 signaling pathway is involved in the survival of CD34(+)/CD38(-)acute myelogenous leukemia cells PMID: 23797738
  30. SNP rs16967637 in the STAT5 gene was the only SNP associated with Crohn's disease without enteritis; homozygosity for the at-risk allele demonstrated the strongest association with this phenotype; results suggest a role for this SNP in development of inflammatory bowel disease of the large intestine PMID: 23929016
  31. aligned IL-2 regulated genes detected by Affymetrix gene expression microarrays with the STAT5 cistrome identified by chip-on-ChIP analysis in an IL-2-dependent human leukemia cell line, Kit225 PMID: 23451206
  32. STAT5B-RARA positive acute promyelocytic leukemia variant is unresponsive to both all trans retinoic acid and arsenic trioxide. PMID: 23271512
  33. This is the first time somatic STAT5 mutations are discovered in human cancer and further emphasizes the role of STAT family genes in the pathogenesis of large granular lymphocytic leukemia. PMID: 23596048
  34. Human STAT5b A630P mutation, like STAT5b F646S is prone to aggregation, as evidenced by its detection in the insoluble fraction, the presence of dimers and higher-order proto-aggregates. PMID: 23160480
  35. Protein level of the signal transducer and activator of transcription, STAT5b, is transiently decreased at temperatures above 37 degrees C. PMID: 23166650
  36. Data suggest that cross-talk occurs between cAMP/PKA and the IL-2R beta/Jak3/Stat5b cascade in T-cells. PMID: 23341462
  37. High STAT5b expression is associated with tumor growth, angiogenesis, and metastases in pancreatic cancer. PMID: 23097626
  38. No association has been found between the STAT5b rs6503691 (C>T) single nucleotide polymorphisms and myeloproliferative neoplasms. PMID: 23130760
  39. These data suggest that STAT5AB is required for the self-renewal of leukemic stem cells PMID: 23149921
  40. results delineate a novel association of nuclear DeltaEGFR with STAT5b, which promotes oncogenesis and treatment resistance in glioblastoma by direct regulation of anti-apoptotic gene, Bcl-XL PMID: 22729867
  41. results point out to a hitherto undescribed link between STAT5 and oxidative stress and provide new insights into STAT5 functions and their roles in leukemogenesis PMID: 22522791
  42. NR0B2 physically interacted with STAT5 and inhibited STAT5 recruitment on the PEPCK gene promoter. PMID: 22977252
  43. pSTAT5 expression was significantly associated with an increased risk of death and of relapse after achieving complete remission PMID: 22725130
  44. Heterozygous STAT5B mutations, with or without heterozygous IGFALS defects, may be associated with growth hormone insensitivity. PMID: 22678306
  45. STAT5b pathway regulates Hsp90alpha expression under hypoxic conditions PMID: 22552610
  46. Ser-193 phosphorylation is associated with Stat5b proto-oncogenic activity and therefore may serve as a novel therapeutic target for treating hematopoietic malignancies. PMID: 22442148
  47. STAT5 phosphorylation levels of EPO and TPO receptors are elevated in bone marrow cells of patients with paroxysmal nocturnal hemoglobinuria. PMID: 22093990
  48. a novel negative cross talk between the NFAT1- and Stat5-signaling cascades that may affect breast tumor formation, growth, and metastasis PMID: 21964595
  49. Stat5-dependent transcriptional regulation is displaced by strong cytosolic iron starvation status induced by mitochondrial ferritin. PMID: 21712541
  50. Genetic variants in STAT5B are associated with total cholesterol and low-density lipoprotein cholesterol levels among six populations. PMID: 21752895
  51. STAT5 regulates glioma cell invasion via at least two different pathways: one that is dependent of STAT5 DNA binding, and a second pathway independent of STAT5 DNA binding. PMID: 20969921
  52. Results indicate an important role for SHP2 in STAT5 activation and GF-mediated proliferation, survival, and differentiation of CD34+ progenitor cells. PMID: 21670473
  53. STAT5B may play a role in regulating the cholesterol metabolism in children with growth hormone deficiency PMID: 21470284
  54. Results describe an open, mass spectrometry-based approach to identify tyrosine phosphorylations associated with AEGFR, and have identified STAT5 as a new target for this receptor. PMID: 21214269
  55. hTid1 negatively regulates the expression and transcriptional activity of STAT5b and suppresses the growth of hematopoietic cells transformed by an oncogenic form of STAT5b. PMID: 21106534
  56. STAT5 expression was elevated in nasal NK/T cell lymphoma. PMID: 17225522
  57. This review focuses on recent discoveries related to the role of STAT5 in the development, function, and transformation of B and T lymphocytes, and its activity as a transcriptional repressor. PMID: 21276004
  58. ZFP36L1 negatively regulates erythroid differentiation by directly binding the 3' untranslated region of Stat5b encoding mRNA. PMID: 20702587
  59. Proteomic analysis identified RELA and STAT5 as two major proteins associated with carboplatin resistance in ovarian tumors PMID: 20585448
  60. Hemin inhibits cyclin D1 and IGF-1 expression via STAT5b under hypoxia in ERalpha-negative MDA-MB 231 breast cancer cells PMID: 20372799
  61. a novel frameshift mutation in STAT5B in 2 male siblings with GH insensitivity (GHI), immunologic defects and elevated prolactin; GHI associated with immune dysfunction, and hyperprolactinemia is strongly suggestive of mutation in STAT5B in both sexes PMID: 20538865
  62. Exercise and fasting increase GH-dependent myocellular STAT5b phosphorylation. PMID: 20534752
  63. Data demonstrate that IL-7 and IL-15 signaling converge on STAT5 to maintain effector CD8(+) T cell responses. PMID: 20644163
  64. STAT5 plays a key role in the generation of B cell precursor acute lymphoblastic leukemia (Review) PMID: 20227268
  65. these results suggest that the JAK2V617F/STAT5 signaling pathway promotes cell proliferation through the transcriptional activation of PTTG1. PMID: 20621061
  66. Data show that exosomes inhibited the expression of JAK3 and phosphorylation of STAT5 in high doses in T-cells, but not JAK2, while EXO/IL-12 had much less attenuated reduction of the expression of p-STAT5. PMID: 20372811
  67. In renal cell carcinoma patients, a decreased proliferative response to tumor, associated with defects in JAK3/STAT5/6 expression that led to increased p27KIP1 expression and alterations in the cell cycle, was observed. PMID: 20339477
  68. These finding indicate a preferential role for STAT5 protein in proliferation of leukemia K562 cells. PMID: 20352702
  69. study of FLT3-associated biological parameters in acute myeloid leukaemia; phospho-STAT5 was reduced in FLT3-internal tandem duplication(ITD)/cytoplasmic nucleophosmin NPMc+ mutation group suggesting NPMc+ may oppose FLT3-ITD-dependent activation of STAT5 PMID: 19775300
  70. STAT5b is integral to breast cancer cell migration. A novel, SH2-dependent function of STAT5b regulates beta1-integrin-mediated migration of highly aggressive breast cancer cells. PMID: 19630967
  71. Results suggest that the NFkappaB regulator BCL10 is an IL-2-independent STAT5 target gene. PMID: 19709433
  72. The data presented here demonstrate that, in contrast to activation by the cytokine, growth hormone (GH), the activation of STAT5b by the growth factor, epidermal growth factor (EGF), requires overexpression of the EGF receptor (EGFR). PMID: 11751923
  73. Interactions of STAT5b-RARalpha, a novel acute promyelocytic leukemia fusion protein, with STAT3 and STAT5 signaling pathways. PMID: 11929748
  74. Oligomerization of the coiled-coil domain of Stat5 in the Stat5-RARalpha fusion protein leads to stable binding of the corepressor SMRT and is accompanied by an impaired response to differentiation signals in hematopoietic cell PMID: 11929749
  75. Up-regulation of RFC2 and the kinase pim-1 by BCR/ABL requires activation of STAT5b by signaling from SH3+SH2 domains of BCR/ABL. PMID: 12036885
  76. Our study thus suggests a functional cooperation between AR and Stat5. PMID: 12089361
  77. No evidence was found for STAT5b rearrangement at the chromosomal or molecular levels in primary leukemias. STAT5 activation is probably a secondary event in most leukemias. PMID: 12145702
  78. CPAP was found to augment Stat5-mediated transcription. PMID: 12198240
  79. role in mediating synergism between c-src and epidermal growth factor receptor PMID: 12429742
  80. role in suppressing transcriptional activity of estrogen receptors and inducing apoptosis in breast cancer cells PMID: 12642867
  81. In tissues from 33 individuals with head and neck cancer, Stat5 activation levels were correlated with progression to a malignant phenotype. PMID: 14583472
  82. Mpl-L-treated Stat5b-deficient mice demonstrated significantly delayed hematopoietic recovery, while Stat5b-deficient mice did not PMID: 14662325
  83. Crossing the STAT5b constitutively active transgene onto the IL-7 receptor-deficient background restores immature B and mature B cell populations as seen by significant increases of immature B and mature B cells. PMID: 15067053
  84. Stat5B is activated by pathways involving FGFR3 and Pyk2 PMID: 15105428
  85. requisite role of the Jak2/Stat5B pathway in mediating episodic growth hormone regulation of CYP2C11 PMID: 15591245
  86. Stat5 activation results in the induction of pax5 and bcl-xL in early thymic lymphoid progenitor cells and thereby redirects these cells down the B cell lineage. PMID: 15944278
  87. STAT5b may mediate the transcriptional activation of cyclin D1 after hypoxic stimulation. PMID: 16155412
  88. IFNalpha induces the activation of STAT5 in melanoma cells, and in STAT5-overexpressing cells, this contributes to IFNalpha resistance. PMID: 16169484
  89. Stat5b A630P mutant is an inactive transcription factor by virtue of its aberrant folding and diminished solubility triggered by a misfolded SH2 domain. PMID: 16303763
  90. STAT5b is an inefficient signal transducer and transcription factor, the detrimental impact on signaling pathways important for normal growth and immunity explains, in part, the complex clinical phenotype of GH insensitivity and immune dysfunction PMID: 16464942
  91. STAT5b and HNF4alpha exhibit bi-directional cross-talk that may augment HNF4alpha-dependent gene transcription while inhibiting STAT5b transcriptional activity via the inhibitory effects of HNF4alpha on JAK2 phosphorylation PMID: 16584384
  92. Cytoskeleton plays an important role in STAT5 activation and translocation into the nucleus in MCF7 cells stimulated with EGF. PMID: 16765629
  93. STAT5b activity in breast cancer cells is modulated by various mutations of tyrosines within the transactivation domain. PMID: 16772534
  94. Transgenic constitutively activated STAT5b-CA naive CD4+ T cells display a 4-fold greater expression of IL-15 receptor alpha chain on their surface when compared with wild-type T cells. PMID: 16887981
  95. STAT5b propagates an important IL-2-mediated signal for the in vivo accumulation of functional regulatory T cells PMID: 16920911
  96. Therapeutic activation of the GH:STAT5b axis therefore represents a novel target for restoring both normal anabolic metabolism and mucosal tolerance in CD. PMID: 17148664
  97. Defective STAT5b is an etiology for IGF deficiency and the growth hormone insensitivity phenotype. PMID: 17389811
  98. in addition to growth arrest and induced differentiation, OSM also sensitizes normal and transformed osteoblasts to apoptosis by a mechanism implicating (i) activation and nuclear translocation of STAT5 and p53 and (ii) an increased Bax/Bcl-2 ratio PMID: 17471233
  99. IGF-1 expression is activated by hypoxia through STAT5b in human HepG2 cells PMID: 17509524
  100. STAT5 expression is required for the maintenance and expansion of primitive hematopoietic stem and progenitor cells, both in normal as well as leukemic hematopoiesis. PMID: 17630355

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Involvement in disease Growth hormone insensitivity with immunodeficiency (GHII)
Subcellular Location Cytoplasm, Nucleus
Protein Families Transcription factor STAT family
Database Links

HGNC: 11367

OMIM: 245590

KEGG: hsa:6777

STRING: 9606.ENSP00000293328

UniGene: Hs.595276

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