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| Code | CSB-EP007717HU |
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CUSABIO synthesized the recombinant gene by integrating the N-terminal 6xHis-SUMO tag sequence into the targeted gene encoding the 24-265aa of the human EPCAM. The synthesized gene was subsequently cloned into an expression vector. After cloning, the expression vector was introduced into the E.coli for expression. The product was purified to obtain the recombinant human EPCAM protein carrying N-terminal 6xHis-SUMO tag. The SDS-PAGE assayed the purity of this recombinant EPCAM protein greater than 90%. This EPCAM protein migrated along the gel to a band of about 43 kDa molecular weight.
EPCAM is a gene providing instruction of making a protein named epithelial cell adhesion molecule (also abbreviated as Ep-CAM and CD326) in human and belongs to EPCAM family. CD326, a transmembrane glycoprotein, plays an important role in the occurrence and development of colorectal cancer. CD326 is a mutifunctional molecule, including accelerating the cell cycle, promoting cell proliferation, differentiation, migration and immune escape. Furthermore, most tumors have EpCAM expression, and most epithelial tumors show high expression, non-epithelial tumors express weakly or not, and some mesenchymal tumors only show weak positive.
Applications : Control proteins
Review: Fluorescence intensity (at the emission wavelength of 517.6 nm) of the sensor in the presence of MB (5 ng mL -1 ), CD63 (50 ng mL -1 ), BSA (50 ng mL -1 ), EPCAM (50 ng mL -1 ), VEGF (50 ng mL -1 ) and black, respectively. Error bars: SD, n = 3.
By Anonymous
Applications : Drug related studies
Review: Fluorescence intensity (at the emission wavelength of 519 nm) of the sensor in the presence of MUC1 (5 ng mL−1), EpCAM (50 ng mL−1), BSA (50 ng mL−1), PSA (50 ng mL−1), VEGF (50 ng mL−1) and black, respectively. In the presence of other control proteins (50 ng mL−1), the significant increase of fluorescence signal is observed in the presence of the MUC1 (5 ng mL−1), indicating that this proposed strategy exhibited good specificity for MUC1 detection.
By Anonymous
Applications : As control proteins
Review: To verify the specificity of the sensor for CEA detection, control experiments were carried out by using BSA, PSA, CD86 and EpCAM as control proteins. As shown in Fig. 4C, an obvious current was obtained in buffer containing CEA while only negligible currents were obtained in control groups containing BSA, PSA, CD86 and EpCAM even at a 10-fold concentration of CEA, indicating the specificity of the sensor.
By Anonymous
Applications : Protein-protein interaction
Review: there is no significant difference between the current response values of these four groups of proteins (myoglobin, EpCAM, CRP, and CEA) and the blank control group (the difference can be ignored). But he sensing platform has an excellent specificity towards the detection of cTnI.
By Anonymous
