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E.coli-expressed L-dopachrome tautomerase (DCT) CSB-EP006562HU is a recombinant fusion protein of partial DCT coupled to a 10xHis-SUMO-tag at the N-terminus and a Myc-tag at the C-terminus. This protein consists of the topological domain (23-108aa) of human DCT and has a calculated molecular weight of 71.3 kDa. The SDS-PAGE showed an about 80 kDa molecular mass of this protein and determined the purity of over 90%. In-stock DCT proteins are offered now. In addition to producing specific antibodies, this recombinant DCT protein may be used in the studies of melanogenesis.
L-DCT or TRP-2 is an important regulatory enzyme involved in melanin synthesis. It also executes proliferative, immunogenic, and anti-apoptotic roles in melanoma cells. L-DCT/TRP-2 is also a marker for amelanotic and melanotic melanoma. Studies showed that A3B5 (N-(3,5-dimethylphenyl)-3-methoxybenzamide) effectively inhibited melanin production by the degradation of TRP-2.
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I was hoping you can supply more information about the expression of this protein in E.coli. Was it expressed as a soluble construct or from inclusion bodies with refolding? I am trying to express my own protein however I cannot get soluble protein and my protein does not work as well as the protein I bought from you. Are you able to help with this information? CSB-EP006562HU Recombinant Human L-dopachrome tautomerase (DCT)