Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5(LGR5),partial

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Code CSB-EP012906HU
Size US$1726
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names LGR5
Uniprot No. O75473
Research Area others
Alternative Names FEX; G protein coupled receptor 49; G protein coupled receptor 67; g protein-coupled receptor fex; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; GPCR GPR49; GPR 49; GPR 67; GPR49; GPR67; GRP 49; GRP49; HG 38; HG38; Leucine rich repeat containing G protein coupled receptor 5 ; Leucine-rich repeat-containing G-protein coupled receptor 5; LGR 5; LGR5; LGR5_HUMAN; MGC117008; Orphan G protein coupled receptor HG38
Species Homo sapiens (Human)
Source E.coli
Expression Region 22-561aa
Target Protein Sequence GSSPRSGVLLRGCPTHCHCEPDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRHVPTEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTEIPVQAFRSLSALQAMTLALNKIHHIPDYAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELGFHSNNIRSIPEKAFVGNPSLITIHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLESLTLTGAQISSLPQTVCNQLPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIKVDTFQQLLSLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPITGLHGLTHLKLTGNHALQSLISSENFPELKVIEMPYAYQCCAFGVCENAYKISNQWNKGDNSSMDDLHKKDAGMFQAQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDGWLIR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 65.4kDa
Protein Length Extracellular Domain
Tag Info N-terminal 10xHis-tagged and C-terminal myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Gene References into Functions
  1. Data conclude that LGR5 expression in CTCs may serve as a marker for CRC metastasis. PMID: 29949050
  2. LGR5 is a new functional GSC marker and prognostic indicator that can promote EMT by activating the Wnt/beta-catenin pathway and would thus be a novel therapeutic target for glioma. PMID: 30208924
  3. Data found LGR5 as a potential target of miR-340. LGR5 was highly expressed in breast cancer cells. Relative expression of LGR5 was negatively regulated by miR-340. Knockdown of LGR5 also inhibited cell proliferation and drug resistance to docetaxel with enhanced cell apoptosis of breast cancer cells. PMID: 30300682
  4. knockdown of CASC15 significantly inhibited the proliferation, migration and invasion of colon cancer cells in vitro and in vivo. Following mechanistic experiments, CASC15 was observed to act as a sponge to suppress microRNA (miR)4310 that targeted LGR5. PMID: 29956772
  5. LGR5 overexpression was associated with high T stage and LN metastasis status in breast cancer. High LGR5 expression was also associated with reduced relapse-free survival, indicating that LGR5 may represent a promising prognostic marker for breast cancer patients. PMID: 29471794
  6. LGR5 is a critical effector of DDX1 in colorectal cancer cells. PMID: 29869821
  7. Logarithmic expansion of LGR5-expressing cells was observed in human colorectal cancer. PMID: 28958617
  8. Our findings indicated that the Lgr5High/DCLK1High expression pattern may be considered as a signature phenotype for intestinal subtypes of gastric carcinoma. PMID: 28946555
  9. Findings suggest a tumor suppressor role for miR-216a in gliomas, which inhibits glioma cell proliferation, migration, and invasion by targeting LGR5. PMID: 28256193
  10. LGR5 promotes cancer stem cell traits and chemoresistance in cervical cancer. PMID: 28880275
  11. LGR5 expression in the epithelium and stroma was associated with tumor stage, and by integrating functional experiments with LGR5-sorted cell RNA sequencing data from adenoma and normal organoids, there were correlations between LGR5 and CRC-specific genes, including dickkopf WNT signaling pathway inhibitor 4 (DKK4) and SPARC-related modular calcium binding 2 (SMOC2 PMID: 29467240
  12. LGR5 expression is transiently upregulated during the early stage of cardiomyocyte differentiation from hPSCs, and although LGR5 expression is not required for maintaining hPSCs in the undifferentiated state, knockdown of LGR5 leads to decreased expression of key cardiac transcription factors at the early stage with eventual lack of robust beating cardiomyocytes at the late stage. PMID: 28793247
  13. Lgr5 plays important roles in angiogenesis. Lgr5-specific siRNA could be designed into an effective therapeutic agent to inhibit gastric cancer angiogenesis. PMID: 28404940
  14. A regulatory mechanism of LGR5 expression in gastric carcinogenesis, with SP1 as an important component of the transcriptional complex and LGR5 activity, which is modulated by its ligands RSPO1 and RSPO2, whose expression is modulated by methylation. PMID: 28219935
  15. these results demonstrate that Lgr5 activation of Wnt/beta-catenin signaling is a potential mechanism to promote the progression of ESCC and ESCC stem cell renewal, and Lgr5 may be used as a molecular target for the development of treatments for ESCC. PMID: 28404917
  16. This study uncovers a novel cross-talk between LGR5 and TGFbeta signaling in colon cancer and identifies LGR5 as a new modulator of TGFbeta signaling able to suppress colon cancer metastasis. PMID: 28939678
  17. Data show that IKKalpha directly bind to the promoters of LGR5, in turn, upregulating LGR5 expression through activation of STAT3 signaling pathway during cancer progression. PMID: 27049829
  18. Report the presence of aberrantly located LGR5(+) cells coexpressing epithelial markers in the stromal compartment of women with endometriosis. These cells have a statistically significantly different expression profile in deep-infiltrating endometriosis in comparison with other types of endometriosis, independent of the menstrual cycle phase. PMID: 28923287
  19. Arrb1 reduced the chemotherapy-induced Lgr5 stem cell apoptosis by inhibiting endoplasmic reticulum stress-mediated mitochondrial apoptotic signaling. PMID: 27195676
  20. LGR5 signaling in gastric cancer cells.LGR5 crosstalk with FOXO1 in gastric cancer cells. PMID: 28970066
  21. High LGR5 expression is associated with osteoarthritis. PMID: 28777797
  22. The LGR5 primarily functions via the IQGAP1-Rac1 pathway to strengthen cell-cell adhesion in normal adult crypt stem cells and colon cancer cells. PMID: 28739799
  23. Lgr5(+) chief cells are involved in maintaining the homeostatic stem cell pool. Define Lgr5(+) chief cells as a major cell-of-origin of gastric cancer. PMID: 28581476
  24. LGR5-expressing fraction of CD54+ cells represents gastric cancer CSCs, in which LGR5 is closely associated with stemness and EMT core genes PMID: 28033430
  25. clathrin-mediated endocytosis inhibition of the leucine-rich G protein-coupled receptor-5 diminishes cell fitness PMID: 28275053
  26. The findings indicated that Lgr5 might contribute to the intestinal metaplasia during gastric carcinogenesis. PMID: 26077638
  27. Endometrial LGR5 is not an endogenous stem cell marker. Instead, LGR5(+) cells appear to be recruited from blood to be part of the stem cell niche at the perivascular microenvironment to activate the endogenous niche. PMID: 27887719
  28. 2 LGR5 splice variants were expressed in human intestine crypt cells; one lacked exon 5 and the other lacked exons 5-8. Only LGR5FL appeared during cell cycle arrest. The transcript variants appeared when the cell cycle was proceeding. LGR5FL-positive cells were negative for Ki-67 and were enriched after chemotherapy. PMID: 27140312
  29. Low expression of LGR5 is associated with gastric cancer. PMID: 26386561
  30. Report targeting LGR5+ tumor stem cells with an antibody-drug conjugate as effective treatment for colon cancer. PMID: 26582901
  31. Lgr5 overexpression was significantly associated with deep invasion of colorectal cancer lymphnode metastasis, distant metastasis and AJCC stage PMID: 26758198
  32. Data suggest that targeting of leucine rich repeat containing G protein-coupled receptor 5 (LGR5) may be of therapeutic benefit for neuroblastomas. PMID: 26517508
  33. This study shows that Lgr5 can be a valuable and reliable prognostic factor of colorectal cancer progression. PMID: 26674601
  34. We conclude that overexpression of LGR5 is associated with markers of tumor aggressiveness in human papillary thyroid cancer PMID: 26416247
  35. The results suggest that trichosanthin may induce apoptosis in glioma cells by targeting LGR5 and repressing the Wnt/betabcatenin signaling pathway PMID: 26397053
  36. These findings indicate that LGR5 not only participates in carcinogenesis but also maintained stemness by activating Wnt/b-catenin signaling in breast cancer. PMID: 26086949
  37. We confirmed in a large and independent study cohort that LGR5 rs17109924 is a predictive genetic biomarker for time to recurrence in patients with colon cancer treated with 5-FU-based adjuvant chemotherapy. PMID: 25665511
  38. lgr5 methylation, through the regulation of lgr5 expression and colorectal CSC differentiation, may constitute a novel prognostic marker for colorectal cancer patients. PMID: 26599100
  39. High resolution crystal structure of an ectodomain variant of human LGR5 (hLGR5ecto) complexed with a signalling competent fragment of mouse Rspo2. PMID: 26123262
  40. Lgr5-positive cells may be cancer stem cells-like cells in gastric cancer. PMID: 26279446
  41. Demonstrate the presence of LGR5-positive cells in limbal epithelial crypts and their decrease in inflamed conditions, which suggests a critical role of this protein during inflammation and its possible use as a marker in normal crypts. PMID: 26097379
  42. Elevated Lgr5 expression might contribute to the development and progression of colorectal cancer. PMID: 24751002
  43. data reveal a RSPO2-induced, LGR5-dependent Wnt signaling-negative feedback loop that exerts a net growth-suppressive effect on CRC cells PMID: 24476626
  44. our data highlight a potential role of Lgr5-positive cells in the tumorigenesis of colorectal cancer (CRC), and suggest that treating these Lgr5-positive cells in CRCs may substantially improve the outcome of CRC therapy PMID: 25835970
  45. Lgr5 may play an important role in the development and progression of cervical carcinoma. PMID: 25973068
  46. Based on the lack of correlation between Lgr5 and tested cancer stem cell markers, Lgr5 does not seem to be a potential stemness marker or prognostic factor in pancreatic ductal adenocarcinoma. PMID: 25804119
  47. High LGR5 expression was associated with poor prognosis in patients with colorectal cancer(CRC) and that LGR5 is an efficient prognostic factor in CRC. [meta-analysis; review] PMID: 25192390
  48. we suggest that Musashi-1, Lgr5, and pEGFR are overexpressed in human SIAs and may play roles in human SIA carcinogenesis and progression. PMID: 25773390
  49. the higher expression level of DCLK1 in patients undergoing CRT can propose it as a more relevant candidate among CSC markers comparing to Lgr5 for colorectal cancer patients. PMID: 25631749
  50. increased expression of LGR5 during embryogenesis and the neonatal period alter skin development and homeostasis. PMID: 26003047
  51. MicroRNA-100 functions as a tumor suppressor by inhibiting Lgr5 expression in colon cancer cells. PMID: 25483280
  52. High AGR2/LGR5 was associated with poor progression-free survival by multivariate analysis. PMID: 22605983
  53. LGR5 is the most selectively expressed gene in human zona glomerulosa and reduces aldosterone production and cell number. PMID: 25915569
  54. H pylori colonize and manipulate the progenitor and Lgr5(+)stem cell compartments, which alters turnover kinetics and glandular hyperplasia. PMID: 25725293
  55. LGR5 can promote proliferation and tumor formation in cervical cancer cells by activating the Wnt/beta-catenin pathway. PMID: 25193857
  56. Expression of LGR5 and ALDH1A1 was associated with the tumorigenicity, metastasis and poor prognosis of NSCLC, and LGR5 cells in NSCLC were likely have stem cell-like properties due to the significant correlation between LGR5 and ALDH1A1. PMID: 25881507
  57. results suggest that SOX9-mediated transcriptional regulation of LGR5 is critical for the tumorigenicity of glioblastoma cells PMID: 25770425
  58. LGR5 is highly expressed in motor neurons in nervous system and is involved in their development by regulating transcription factors that program motor neuron identity. PMID: 25451725
  59. the findings of this study suggest that LGR5 plays a vital role in CRC pathogenesis and has the potential to serve as a diagnostic marker and a therapeutic target for CRC patients. PMID: 25025569
  60. Nutrient stress within a tumour microenvironment has the capacity to alter LGR5 protein stability and membrane localisation through modulation of LGR5 glycosylation status. PMID: 25611300
  61. High LGR5 expression is associated with colorectal cancer. PMID: 25060070
  62. The stem cell-expressed receptor Lgr5 possesses canonical and functionally active molecular determinants critical to beta-arrestin-2 recruitment. PMID: 24386388
  63. Study demonstrated that elevated Lgr5 expression in gastric cancer following preoperative chemotherapy is significantly associated with tumor progression, poor response to chemotherapy and shorter survival time. PMID: 24859092
  64. LGR5 is required for the maintenance of spheroid-derived colon cancer stem cells. PMID: 24789370
  65. This review discusses the discovery of LGR5 and the impact it is having on our understanding of stem cell and cancer biology of the colon. [review] PMID: 24677446
  66. Results show that overexpression of Lgr5 was significantly correlated with lymph node metastasis, tumor stage, and response to chemotherapy and appeared to be associated with poorer survival in patients with small cell carcinoma of the esophagus. PMID: 24666414
  67. Lgr5(+) cells are seen in a large number of gastric tumors ; their frequent basal arrangements and coexpression of ISC markers support the idea that Lgr5(+) cells act as stem cells during the early stage of intestinal-type gastric tumorigenesis. PMID: 24340024
  68. data demonstrated a correlation between LGR5 expression and the proliferation index in glioma, and knockdown of LGR5 resulted in suppression of glioma cell proliferation in vitro and in vivo. PMID: 24172981
  69. Intensity of LGR5(+) staining showed an increasing trend that generally followed increasing dedifferentiation and tumor spread. PMID: 24379591
  70. Leucine-rich repeat-containing G-protein-coupled receptor 5 was correlated with invasion and metastasis. Leucine-rich repeat-containing G-protein-coupled receptor 5 inhibition could serve as a novel therapeutic approach. PMID: 24594994
  71. LGR5 expression may be functionally correlated with the neurogenic competence, and be regulated by OLIG2 in stem-like cells in glioblastoma. PMID: 23793848
  72. data elucidate mechanisms that define the role of LGR5 as a marker for stem-like cells in CRC PMID: 24282287
  73. Findings provide new insights into the underlying homeostatic regulation of human corneal endothelial stem/progenitor cells by LGR5 through the HH and Wnt pathways. PMID: 23553870
  74. Elevated Lgr5 level is associated with colorectal cancer progression and treatment response. PMID: 23784051
  75. Our results indicate that Lgr5 may mark adult taste stem or progenitor cells in the posterior portion of the tongue. PMID: 23377989
  76. Results show that LGR5 is coupled to the Rho pathway through G12/13 signaling PMID: 23912594
  77. Lgr5 is expressed in a subset of lung adenocarcinoma, and its expression is related to some clinicopathological parameters and a poorer prognosis. PMID: 23915911
  78. All our study manifested that LGR5 took on an important effect in the initiation and progression of colon cancer, provided also more helpful evidence for clinical diagnosis and an useful indicator for adjuvant therapy. PMID: 24063790
  79. Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5) as a putative human endometrial stem cell marker. PMID: 23475985
  80. Lgr5 and Bmi1 were co-expressed within the same cells of gastric glands. PMID: 23664892
  81. MiR-142-3p functions as a tumor suppressor by targeting CD133, ABCG2, and Lgr5 in colon cancer cells PMID: 23619912
  82. Expression of Nanog and LGR5 in normal ovaries and in borderline tumors may assist in the early detection and improved prognosis of ovarian cancer PMID: 23092806
  83. LGR5 small interfering RNA inhibited the survival-promoting effects of PGE2 in RG/C2. PMID: 23349017
  84. Both LGR5(+) and LGR5(-) cells were detected in the tumor tissues of colon cancer patients. PMID: 23081779
  85. TROY is up-regulated in human colorectal cancer cell lines and in intestinal tumors in mice. It functions as a negative modulator of the Wnt pathway in LGR5-positive stem cells. PMID: 23142137
  86. Leucine-rich repeat-containing G protein-coupled receptor 5 are overexpressed in gastric cancer. PMID: 23356221
  87. The constitutive internalization of LGR5 to the TGN suggests the existence of novel biochemical roles for its Wnt pathway related, but ill defined signaling program. PMID: 23439653
  88. the islet's beta cells, expressing LGR5 and Nanog markers are the initiating cells of pancreas cancer and LGR5 is a major biomarker for pancreatic ductal adenocarcinomas PMID: 23438436
  89. The results of this study found suggest that LGR5 plays a role in maintenance and/or survival of brain cancer stem-like cells. PMID: 22805276
  90. In gastric cancer patients with an active H. pylori infection, Lgr5-positive epithelial cells may be more susceptible to DNA damage than Lgr5-negative cells. PMID: 22945475
  91. Lgr5 expression is dependent on the Wnt pathway and overexpression induces clonogenic growth. PMID: 22969042
  92. LGR5 and CD44 expression may be coordinately associated with tumor relapse in locally advanced rectal cancer after preoperative chemoradiotherapy (CRT). PMID: 22923071
  93. Aberrant expression of LGR5 regulates epithelial cell phenotype and survival. PMID: 23127514
  94. Lgr5 expression remained restricted to cell clusters within developing nephrons in the cortex until postnatal day 7, when expression was permanently silenced. PMID: 22999937
  95. Results suggest that LGR5 expression may be implicated in resistance to chemoradiotherapy (CRT), therefore contributing to tumor relapse in patients with locally advanced rectal cancer treated with preoperative CRT. PMID: 22986809
  96. LGR5 is a part of the Wnt signaling complex at the membrane level to enhance Wnt/beta-catenin signaling. However, internalization of LGR5 does not appear to be essential for potentiating the canonical Wnt signaling pathway. PMID: 22473993
  97. LGR5 is differentially expressed in gastrointestinal cancers and that the spatial histoanatomical distribution of LGR5(+) cells has to be considered when their tumour biological significance is sought. PMID: 22530031
  98. LGR5 expression can be related to cellular stemness and can be considered as a new phenotypic marker of residual human corneal limbal stem cells. PMID: 22322201
  99. An attenuated mRNA level of the newly identified transcript variant GPR49Delta5 is a negative prognostic marker for disease-associated and recurrence-free survival in STS patients. PMID: 21978106
  100. Loss of LGR5 is associated with colorectal cancer. PMID: 21829496

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Subcellular Location Cell membrane, Multi-pass membrane protein, Golgi apparatus, trans-Golgi network membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of th
Database Links

HGNC: 4504

OMIM: 606667

KEGG: hsa:8549

STRING: 9606.ENSP00000266674

UniGene: Hs.658889

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