LGR5

CSB-MP012906HU(A4) is detected by Mouse anti-10*His monoclonal antibody.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human LGR5 at 10 μg/ml can bind Anti-LGR5 recombinant antibody (CSB-RA012906MA1HU). The EC₅₀ is 1.690-1.867 ng/mL.The VLPs (CSB-MP3838) is negative control.

Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue
All lanes: LGR5 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 100, 98, 93 kDa
Observed band size: 100 kDa

Overlay histogram showing HepG2 cells stained with CSB-RA262034A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*10⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.

IHC image of CSB-PA012906LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA012906LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).