The Recombinant protein with an MBP-tag at N-terminal and a 6xHis-tag at the C-terminal corresponds to the amino acids 22-555 of Human Perforin-1/PRF1 full-length ORF. This Baculovirus-expressed PRF1 protein got purified by SDS-PAGE and reached up to 85% in purity. On a reducing SDS–PAGE gel, the PRF1 protein migrated to about 105kDa. Customers can get the protein faster because it has stock. The recombinant full-length of mature PRF1 protein may be used for antibody synthesis or in the immunological research related to PRF1.
PRF1 is a Ca2+-dependent glycoprotein mainly expressed by natural killer (NK)cells and CD8-positive T-cells. Polymerization of PRF1 forms channels on the target cell, thus allowing for the passive diffusion of granzymes into the cell. The cellular cytotoxicity mediated by PRF1 is responsible for killing virus-infected and neoplastic cells. Mutations in the PRF1 gene are associated with familial hemophagocytic lymphohistiocytosis (FHL) and various types of lymphomas.
||Greater than 85% as determined by SDS-PAGE.
||Cytolysin; FLH2; HPLH2; Lymphocyte pore-forming protein; P1; PERF_HUMAN; perforin 1 (pore forming protein); Perforin 1; Perforin-1; PFP; PGFL; PIGF; PIGF-2; PLGF; Pore forming protein; prf1; SHGC-10760
||Homo sapiens (Human)
|Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
||Full Length of Mature Protein
N-terminal MBP-tagged and C-terminal 6xHis-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
||3-7 business days
||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
|Datasheet & COA
|| Please contact us to get it.