Very nice to receive your inquiry. The product details are confirmed as below.
Name: Recombinant Human Perforin-1(PRF1)
Expression Region: 22-555aa; Full Length of Mature Protein
Tag Info: N-terminal 6xHis-SUMO-tagged
Product Type: Developed Recombinant Protein
We have two lots of temporary stock for this protein. The details are as below.
1) Delivery Form: Liquid; Storage Buffer: 10 mM Tris-HCl,1 mM EDTA, pH 8.0, 20% glycerol; Endotoxin Removal: No; Aseptic Processing: No;
2) Delivery Form: Lyophilized Powder; Packing Size: 200ug/vial;
(If your order size is 200ug or 200ug*X, you could choose lyophilized powder, if not, we suggest liquid form.)
We haven't performed Endotoxin Removal for both Liquid Form and Lyophilized Powder. If you have demand for endotoxin level, please remark this requirement in your PO,
and we could guarantee Endotoxin Level <1.0 EU per 1μg of the protein by the LAL method.
We haven't validated the biologically activity of this protein. There is literature reporting that the full-length protein recombinantly expressed by the insect baculovirus system (BP) has quite good Cytolytic activity
(Needs neutral pH conditions and certain calcium ion concentrations). (Biochemical and functional characterization showed the purified full-length recombinant perforin to be capable of lysing target cells,
inducing Ca2+ influx into target cells, and forming structural pores in target membranes). Here is the link of the literature: http://www.jimmunol.org/content/156/9/3292.abstract
The protein we provide is the full length of mature protein which has complete structure, so our protein should have good activity in theory. We suggest you to purchase small size to do validation first.
If the protein is active after your validation, you could purchase larger size later, and we will provide certain discount for your re-order.