Recombinant Mouse Microtubule-associated protein tau(Mapt)


In Stock
Code CSB-YP013481MO
Product Type Recombinant Protein
Size US$2959Purchase it in Cusabio online store
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Uniprot No. P10637
Relevance Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP013481MO could indicate that this peptide derived from Yeast-expressed Mus musculus (Mouse) Mapt.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP013481MO could indicate that this peptide derived from Yeast-expressed Mus musculus (Mouse) Mapt.
Storage Buffer Tris-based buffer,50% glycerol
Alias Neurofibrillary tangle protein Paired helical filament-tau Short name: PHF-tau
Species Mus musculus (Mouse)
Purity Greater than 90% as determined by SDS-PAGE.
Sequence ADPRQEFDTMEDHAGDYTLLQDQEGDMDHGLKESPPQPPADDGAEEPGSETSDAKSTPTAEDVTAPLVDERAPDKQAAAQPHTEIPEGITAEEAGIGDTPNQEDQAAGHVTQGRREGQAPDLGTSDWTRQQVSSMSGAPLLPQGLREATCQPSGTRPEDIEKSHPASELLRRGPPQKEGWGQDRLGSEEEVDEDLTVDESSQDSPPSQASLTPGRAAPQAGSGSVCGETASVPGLPTEGSVPLPADFFSKVSAETQASQPEGPGTGPMEEGHEAAPEFTFHVEIKASTPKEQDLEGATVVGVPGEEQKAQTQGPSVGKGTKEASLQEPPGKQPAAGLPGRPVSRVPQLKARVASKDRTGNDEKKAKTSTPSCAKAPSHRPCLSPTRPTLGSSDPLIKPSSPAVSPEPATSPKHVSSVTPRNGSPGTKQMKLKGADGKTGAKIATPRGAASPAQKGTSNATRIPAKTTPSPKTPPGSGEPPKSGERSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSASKSRLQTAPVPMPDLKNVRSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL
Research Area Neuroscience
Source Yeast
Gene Names Mapt
Protein Names Recommended name: Microtubule-associated protein tau Alternative name(s): Neurofibrillary tangle protein Paired helical filament-tau Short name= PHF-tau
Expression Region 2-733aa
Tag Info N-terminal 6xHis-tagged
Mol. Weight 78.1kDa
Protein Description Full Length of Mature Protein
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Function Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Involvement in disease May be involved in the pathogenesis of cytoplasmic inclusions (as Mallory bodies) in livers of mice chronically intoxicated with Griseofulvin or DDC (3,5-diethoxycarbonyl-2,4-dihydrocollidine), a model for human alcoholic hepatitis. Alteration of Tau (abnormal phosphorylation and cross-linking) could contribute to Mallory bodies formation and disturbance of microtubule function in alcoholic liver disease.
Subcellular Location Cytoplasm, cytosol, Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cytoplasm, cytoskeleton, Cell projection, axon
Tissue Specificity Expressed in neurons and at a lower level in the liver and kidney. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
Database Links

KEGG: mmu:17762

STRING: 10090.ENSMUSP00000097919

UniGene: Mm.1287

Pathway MAPK signaling pathway

Q&A and Customer Reviews

Q&A
Q:

We purchased mouse tau antigen product (Mouse MAPT / Tau Protein (Recombinant 6His, N-terminus, aa2-733, CSB-YP013481MO lot #03768). We tested an ELISA experiment with this product. The assay consists of two antibodies (Tau5 and BT2). An obvious signal was found when human tau (2N4R) is used as an antigen, but no signal was found when CSB-YP013481MO is used as an antigen. These two tests were performed on the same plate and same time. The epitope of Tau5 is aa504-511 (PPTREPKK) of L CSB-YP013481MO and the epitope of BT2 is aa480-484 (RSGYS) of CSB-YP013481MO. Both epitopes are exist in human tau and mouse tau. We wonder why mouse antigen CSB-YP013481MO gave no signal on this ELISA. It has questioned the certainty of the product.

A:
Thanks for your feedback.Here are our replies to your questions. Please check.
1. The Tau monoclonal antibody (Tau5 and BT2) you used should be one of the following:
https://www.thermofisher.com/antibody/product/Tau-Antibody-clone-TAU-5-Monoclonal/MA5-12808
https://www.thermofisher.com/antibody/product/Tau-Antibody-clone-TAU-5-Monoclonal/AHB0042
https://www.novusbio.com/products/tau-antibody-tau-5_nb200-514
https://www.thermofisher.com/antibody/product/Tau-Antibody-clone-BT2-Monoclonal/MN1010
From your WB test result: Both of Tau-5 and BT2 recognize the 50kDa band. Tau has 9 isomers, and its standard isomer is Isoform PNS-tau with calculated value of 78.9kDa, which has multiple post-translation modifications (glycosylation/phosphorylation/acetylation). And its observed size must be larger than 80 kDa. This shows that neither of these two antibodies recognize the standard isoform (Isoform PNS-tau).
2. You used Tau (2N4R) as an antigen and the signal was detected. The corresponding information is as below: https://www.rpeptide.com/products/proteins/tau/t-1001-1
It corresponds to the tau-4 isomer (Isoform Tau- F) and its calculated value is 45.8 kDa. Taking the post-translational modification into consideration, its observed size should larger than 45.8 kDa. Combined with the previous two mAb information (Both recognized ~50 kDa band), it is presumed that both of these two mAbs recognize tau-4 isomer (identifier: P10636-8).
3. What we provided was the standard isoform of mouse species: Isoform PNS-Tau (identifier: P10637-1) with a calculated value of 76.2 kDa.
The two monoclonal antibodies did not recognize the standard isoform of human species: Isoform PNS-tau (identifier: P10636-1 ). It is likely that the standard isomers of mouse also can not recognized. The structure of different isomers is not exactly the same, and it is likely that the epitopes recognized by these two mAbs are different in the structure of standard isomers and tau-4 isomers
(existence of entrapment/non-linearity, etc.).
4. We have performed Mass Spectrometry Identification for this protein (CSB-YP013481MO) and have uploaded the MS image onto our website. You could have a look. Here is the link: https://www.cusabio.com/Recombinant-Protein/Recombinant-Mouse-Microtubule-associated-protein-tauMapt-3547.html
5. If you want the mouse antibody against this protein, we can provide customize service for this antibody with preferential price.
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