Code | CSB-YP013481MO |
MSDS | |
Size | $368 |
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Recombinant full-length of mature Mouse Microtubule-associated protein tau (Mapt) cDNA (2-733aa) constructed with a 6xHis-tag at the N-terminus was expressed in the yeast. The protein identity was confirmed by both SDS-PAGE and LC-MS/MS analysis. It is over 90% in purity and has a calculated molecular weight of 78.1 kDa. Importantly, this recombinant Mapt protein is in stock so there is no waiting period for product preparation. The Mapt can act as an immunogen to elicit an adaptive immune reaction and thus obtain specific antibodies. Besides, this protein may also be used in the studies of the neuroscience field.
Mapt is abundant in the axons of neurons where it promotes microtubule (MT) assembly and stability. Together with stathmin and other destabilizing MAPs, Mapt also participates in MT dynamics via the modulation of assembly, dynamic behavior, and the spatial construction of MTs. Aberrant hyperphosphorylation of Mapt-causing its self-aggregation into paired helical filaments and buildup in the neurons frequently occurred in the tauopathies such as Alzheimer's disease (AD) and frontotemporal dementia (FTD).
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We purchased mouse tau antigen product (Mouse MAPT / Tau Protein (Recombinant 6His, N-terminus, aa2-733, CSB-YP013481MO lot #03768). We tested an ELISA experiment with this product. The assay consists of two antibodies (Tau5 and BT2). An obvious signal was found when human tau (2N4R) is used as an antigen, but no signal was found when CSB-YP013481MO is used as an antigen. These two tests were performed on the same plate and same time. The epitope of Tau5 is aa504-511 (PPTREPKK) of L CSB-YP013481MO and the epitope of BT2 is aa480-484 (RSGYS) of CSB-YP013481MO. Both epitopes are exist in human tau and mouse tau. We wonder why mouse antigen CSB-YP013481MO gave no signal on this ELISA. It has questioned the certainty of the product.