Purity
Greater than 90% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Mapt at 2 μg/mL can bind anti-MAPT recombinant antibody (CSB-RA013481A1HU),the EC50 is 436.1-518.6 ng/mL.
Alternative Names
(Neurofibrillary tangle protein) (Paired helical filament-tau) (PHF-tau)
Molecular Characterization
Species
Mus musculus (Mouse)
Expression Region
2-733aa
Target Protein Sequence
ADPRQEFDTMEDHAGDYTLLQDQEGDMDHGLKESPPQPPADDGAEEPGSETSDAKSTPTAEDVTAPLVDERAPDKQAAAQPHTEIPEGITAEEAGIGDTPNQEDQAAGHVTQGRREGQAPDLGTSDWTRQQVSSMSGAPLLPQGLREATCQPSGTRPEDIEKSHPASELLRRGPPQKEGWGQDRLGSEEEVDEDLTVDESSQDSPPSQASLTPGRAAPQAGSGSVCGETASVPGLPTEGSVPLPADFFSKVSAETQASQPEGPGTGPMEEGHEAAPEFTFHVEIKASTPKEQDLEGATVVGVPGEEQKAQTQGPSVGKGTKEASLQEPPGKQPAAGLPGRPVSRVPQLKARVASKDRTGNDEKKAKTSTPSCAKAPSHRPCLSPTRPTLGSSDPLIKPSSPAVSPEPATSPKHVSSVTPRNGSPGTKQMKLKGADGKTGAKIATPRGAASPAQKGTSNATRIPAKTTPSPKTPPGSGEPPKSGERSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSASKSRLQTAPVPMPDLKNVRSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The gene corresponding to the 2-733aa off the mouse microtubule-associated protein tau (MAPT) was fused with the 10xHis-tagged at the N-terminus and then cloned into a plasmid vector, forming the recombinant vector. Mammalian cells were transfected with the recombinant vector, allowing for its expression. The product was purified to obtain the N-terminal 10xHis-tagged recombinant mouse MAPT protein. Its purity is greater than 90% determined by SDS-PAGE. The endotoxin of this protein is less than 1.0 EU/ug measured by the LAL method. Its activity has also been validated through a functional ELISA. This MAPT protein can bind the anti-MAPT recombinant antibody, with the EC50 of 436.1-518.6 ng/mL.
MAPT protein, also called Tau, is abundant in neurons of the central nervous system (CNS), while relatively low in non-neuron cells like astrocytes and oligodendrocytes. Tau is involved in the regulation of axon transport, the maintenance of nuclear function to protect DNA integrity, the interaction with actin cytoskeleton to promote the formation of actin filaments, and the government of NMDA receptor signaling pathway through interaction with Fyn. Since its involvement in numerous neurodegenerative diseases such as Alzheimer's disease (AD) and primary age-related tau lesions (PART), Tau has become a hot target for therapy of these disorders. In clinical application, Tau protein examination is of great value for the evaluation of AD and PART.