Recombinant Saccharomyces cerevisiae Nuclear polyadenylated RNA-binding protein NAB2 (NAB2), partial

1. Binding A11G RNA induces dimerization of Zn fingers 5-7 mediated by the novel spatial arrangement of the fingers promoting each RNA chain binding two protein chains. The dimerization of Nab2 induced by RNA binding provides a basis for understanding its function in both poly(A) tail length regulation and in the compaction of mature transcripts to facilitate nuclear export. PMID: 28180315
2. Studies provide evidence Nab2 is important for efficient RNAPIII transcription initiation in addition to its known functions in gene expression, i.e., poly(A) tail length control, mRNP biogenesis, and nuclear mRNA export. PMID: 27049816
3. Nab2p uses its last two CCCH domains and its RGG-box to form a Nab2p/poly(A) complex of unusual topology that defines and controls mRNA poly(A) tail length by preventing 3'-end accessibility to the poly(A) polymerase PMID: 18304944
4. absence of Nab2p results in robust nuclear mRNA decay by the ribonucleolytic RNA exosome. PMID: 26119729
5. Nab2 increases TFIIIB binding to the promoter, thereby stimulating RNAPIII transcription. PMID: 26220998
6. Nab2p tandem zinc finger TZF34 exhibits a distinctive two-fold symmetry of the zinc centers with mutual recognition of histidine ligands. PMID: 23994011
7. A seven-residue segment of the PY-NLS of Nab2 is observed to bind Kapbeta2 in an extended conformation and occupies the same PY-NLS binding site observed in other Kapbeta2.PY-NLS structures. PMID: 23535894
8. the solution structure of Nab2 ZnF5-7 and their interaction with polyadenosine-RNA PMID: 22560733
9. nuclear mRNP surveillance step involves targeting of Rrp6p by Nab2p-bound pA-tailed RNPs and that pre-mRNA abundance is regulated at this level PMID: 22683267
10. Nab2 function is a central player in the general and coordinated control of gene expression from transcription to translation. PMID: 21680710
11. Nab2 binding to RNA is critical for proper control of poly(A) tail length. PMID: 20554526
12. data show that Nab2 is preferentially associated with THO/TREX complex members and with many if not all poly(A)+ mRNAs; this supports a model in which Nab2 is involved in general mRNP assembly and export PMID: 19840948
13. N-terminal Nab2 domain is distinct from its RNA binding domains suggesting Nab2 could bind Gfd1 and RNA simultaneously PMID: 15208322
14. these proteins are members of an evolutionarily conserved family of poly(A) RNA binding proteins PMID: 17630287
15. NAB2 functions together with PUB1 to modulate mRNA stability and strengthen a model where nuclear events are coupled to the control of mRNA turnover in cytoplasm. PMID: 17636033
16. The N-terminal domain of Nab2 (residues 1-97) mediates interactions with both the C-terminal globular domain of the nuclear pore-associated protein, myosin-like protein 1 (Mlp1), and the mRNA export factor, Gfd1. PMID: 18190927
17. Functional significance of the interaction between the mRNA-binding protein, Nab2, and the nuclear pore-associated protein, Mlp1, in mRNA export PMID: 18682389

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KEGG: sce:YGL122C

STRING: 4932.YGL122C