Code | CSB-RA005087A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
The production of the CDKN1B recombinant monoclonal antibody involves the utilization of DNA recombinant technology and in vitro genetic manipulation. Initially, animals are immunized with a synthesized peptide derived from human CDKN1B, which triggers an immune response and allows for the isolation of B cells. Following a stringent screening process, positive B cells are identified and selected for further analysis. The genes encoding the light and heavy chains of the CDKN1B antibody are then amplified using PCR and inserted into a plasmid vector, resulting in the creation of a recombinant vector. This recombinant vector is subsequently introduced into host cells, where it directs the expression of the antibody. The CDKN1B recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. This purified antibody exhibits high specificity towards both human and CDKN1B protein and is tested for use in ELISA, WB, IHC, and IF applications.
The CDKN1B protein, also known as p27, plays a critical role in regulating the cell cycle by inhibiting the activity of CDKs. CDKN1B binds to and inhibits the activity of CDK-cyclin complexes, preventing the phosphorylation of key substrates that promote cell cycle progression. This ultimately leads to cell cycle arrest in the G1 phase, which allows for DNA repair, differentiation, or apoptosis. Additionally, CDKN1B is also involved in the regulation of cell migration, adhesion, and apoptosis.
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