CDKN1B Antibody

Code CSB-RA005087A0HU
Size US$350
Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate
    All lanes: CDKN1B antibody at 0.9μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 23 KDa
    Observed band size: 27 KDa

  • IHC image of CSB-RA005087A0HU diluted at 1:97.5 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-RA005087A0HU diluted at 1:97.5 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-RA005087A0HU at 1:32.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

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Product Details

Uniprot No. P46527
Target Names CDKN1B
Alternative Names AA408329 antibody; AI843786 antibody; Cdki1b antibody; CDKN 1B antibody; CDKN 4 antibody; CDKN1B antibody; CDKN4 antibody; CDN1B_HUMAN antibody; Cyclin Dependent Kinase Inhibitor 1B antibody; Cyclin dependent kinase inhibitor p27 antibody; Cyclin-dependent kinase inhibitor 1B (p27; Kip1) antibody; Cyclin-dependent kinase inhibitor 1B antibody; Cyclin-dependent kinase inhibitor p27 antibody; Cyclin-dependent kinase inhibitor p27 Kip1 antibody; KIP 1 antibody; KIP1 antibody; MEN1B antibody; MEN4 antibody; OTTHUMP00000195098 antibody; OTTHUMP00000195099 antibody; p27 antibody; p27 Kip1 antibody; P27-like cyclin-dependent kinase inhibitor antibody; p27Kip1 antibody
Species Reactivity Human, Mouse
Immunogen A synthesized peptide derived from human CDKN1B
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
IF 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA
Gene References into Functions
  1. the p27 gene rs34330 polymorphism might increase the cancer susceptibility, especially in Asians. PMID: 28317869
  2. our study identified that p27 expression was transcriptionally upregulated by enhancing the binding of FOXO1 to its promoter and post-transcriptionally induced through decreasing binding of miR-182 to its mRNA 3'-UTR upon isorhapontigenin treatment PMID: 29409027
  3. Cholangiocarcinoma growth is associated with nuclear export of P27 that is due to AKT-mediated phosphorylation of P27 at T157. PMID: 29428513
  4. Multiple function of p27 in cell cycle regulation, apoptosis, epigenetic modifications and post- translational modification are reviewed, and the mechanisms and factors that have important roles in p27 functions are briefly discussed. PMID: 30075372
  5. Data show that fluid shear stress activates NOTCH signaling, which upregulates GJA4 (commonly, Cx37) and downstream cell cycle inhibitor CDKN1B (p27). PMID: 29247167
  6. High KIP1 expression is associated with multiple endocrine neoplasia type 1-related pancreatic neuroendocrine tumors. PMID: 29134609
  7. Meta-analysis failed to validate any association between p27-V109G and prostate cancer risk. PMID: 29750086
  8. after irradiation, loss of p27 is associated to accumulation of residual DNA damage and increased number of mitotic aberration. We show that p27 expression is necessary to preserve genomic integrity and for correct recognition and clearing-out of aberrant cells. PMID: 28377607
  9. FoxO3a overexpression increased the transcription and protein expression of Bcl2like protein 11 and cyclindependent kinase inhibitor 1B, and inhibited cyclin D1 transcription and expression. PMID: 29257235
  10. Results suggest that CacyBP/SIP plays an important role in inhibiting glioma cell migration and invasion through promoting the degradation of cytoplasmic p27. PMID: 29024247
  11. The functional variant rs34330 of CDKN1B is associated with risk of neuroblastoma. PMID: 28667701
  12. FLT3 and FLT3-ITD can directly bind and selectively phosphorylate p27kip1 on tyrosine residue 88 in acute myeloid leukemia. Inhibition of FLT3-ITD in cell lines strongly reduced p27 tyrosine 88 phosphorylation and resulted in increased p27 levels and cell cycle arrest PMID: 28522571
  13. Our results suggest that oxidised photoreceptor outer segments -induced retinal pigment epithelial cell cytokinesis failure is, at least in part, due to the upregulation of p27kip1 through activating the PKC, particularly PKCzeta pathway PMID: 29016360
  14. p27Kip1-838C>A; rs36228499 is functional in human venous smooth muscle cells (SMCs) and in adventitial cells. The AA genotype is associated with stronger expression of the p27 gene and p27 protein. Only the adventitial cells, and not the SMCs, are responsive to the inhibitory effects of the protective AA genotype in cell growth. PMID: 28526559
  15. Overview of CDKN1B mutations in MEN4 (review) PMID: 28824003
  16. Taken together, these data clearly show that FKBP3/Sp1/HDAC2/p27 control cell proliferation during non-small cell lung cancer development. PMID: 28839465
  17. Sumoylation of K73 site of cyclin-dependent kinase inhibitor 1B (p27kip1) is critical to the nuclear-cytoplasmic translocation of p27kip1, which is mediated by RAN binding protein 2 and CRM1 protein. Sumoylation of p27kip1 promotes the proliferation of cholangiocarcinoma QBC939 cells PMID: 28882106
  18. loss of CDKN1B increased the prevalence of cell cycle regulator defects in immature T-ALL, usually only ascribed to CDKN2A/B deletions, and that CDKN1B deletions frequently coincide with expression of MEF2C, considered as one of the driving oncogenes in immature early T-cell precursor (ETP) ALL PMID: 28482719
  19. This study reveals a molecular pathway involving lncRNA GAS5/E2F1/P27(Kip1) which regulates cell proliferation and could be a potential therapeutic target in prostate cancer. PMID: 28396462
  20. miR-155-5p promotes fibroblast cell proliferation and inhibits FOXO signaling pathway by negative modulation of both FOXO3 and CDKN1B in vulvar lichen sclerosis PMID: 29339071
  21. We demonstrate that E6AP regulates p27 expression by inhibiting its transcription in an E2F1-dependent manner. Concomitant knockdown of E6AP and p27 partially restores PC cell growth, supporting the contribution of p27 to the overall effect of E6AP on prostate tumorigenesis. PMID: 28477016
  22. SIRT1-mediated downregulation of p27Kip1 is essential for overcoming contact inhibition of Kaposi's sarcoma-associated herpesvirus transformed cells PMID: 27708228
  23. we suggest that gastrin contributes to the emergence of MDR of SGC7901 cells via the degradation of p27Kip1 PMID: 28498440
  24. this studies show that p27kip1 is an important regulator of Ras induced neoplastic transformation PMID: 27579539
  25. Loss of p27kip1 expression is frequently found in papillary thyroid carcinoma (PTC) compared with benign lesions and normal thyroid tissue. When present in PTC, it is correlated with aggressive tumor behavior. PMID: 27834461
  26. Overall, the authors find that p27 directly promotes cell invasion by facilitating invadopodia turnover via the Rac1/PAK1/Cortactin pathway. PMID: 28287395
  27. Upon binding to p27(Kip1) 3'UTR, CPEB1 promotes elongation of poly-A tail and the subsequent translation of p27(Kip1) mRNA. This leads to higher levels of p27(Kip1) in the cell, in turn significantly inhibiting cell proliferation, and confers to CPEB1 a potential value as a tumor suppressor in Glioblastoma. PMID: 27142352
  28. p27(kip1) overexpression regulates IL-1beta in the microenvironment of stem cells and eutopic endometriosis PMID: 26817396
  29. Data suggest that NOX5 expression in melanoma cells could contribute to cell proliferation due, in part, to the generation of high local concentrations of extracellular ROS that modulate multiple pathways that regulate HIF-1alpha and networks that signal through Akt/GSK3beta/p27(Kip1) . PMID: 28762556
  30. SNHG6 acted as an oncogene in gastric cancer cells through regulating miR-101-3p/ZEB1 at a post-transcriptional level and silencing expression at a transcriptional level by recruiting enhancer of zeste homolog 2 (EZH2) to the promoter of p27. PMID: 28683446
  31. PCTAIRE1 has a role in regulating p27, c-Myc levels and tumor growth in cutaneous cutaneous squamous cell carcinoma cells PMID: 28274513
  32. Low P27KIP1 expression is associated with Non Small Cell Lung Cancer. PMID: 27880728
  33. Results show that that Id2 was directly upregulated by BMP4, resulting in the mediated expression of cell cycle regulatory protein of CDKN1B. PMID: 28543546
  34. p27 and its cognate ubiquitin ligases, Skp2/KPC/Pirh2, are specifically involved in determining the clinical profiles of lung carcinomas. PMID: 28601655
  35. In thyroid cancer cells, oncogene activation prevented TGF-beta/SMAD-dependent p27 repression, and CDK2/SMAD3 phosphorylation, leading to p65 NFkappaB upregulation which repressed BAX, induced cyclin D1 and promoted TGF-beta-dependent growth. PMID: 27452523
  36. PTEN loss and p27 loss differ among morphologic patterns of prostate cancer. PMID: 28504208
  37. These findings provide a new understanding of the effects of oxLDL on endothelial proliferation, which is essential for developing new treatments against neovascularization and progression of atherosclerosis. PMID: 28701359
  38. abnormal levels of Skp2 and p27(KIP1) have probably been involved in the pathogenesis of ADH and DCIS. Thus, Skp2 and p27(KIP1) may serve as important diagnosis markers. PMID: 28514182
  39. Cip2a markedly decreased the expression and nuclear localization of p27Kip1 and this is critical for the ability of Cip2a to promote Triple-negative breast cancer progression. PMID: 27694903
  40. These results indicate that the dynamic interplay between O-GlcNAcylation and cyclin dependent kinase inhibitor p27 phosphorylation coordinates and regulates cell proliferation in hepatocellular carcinoma. PMID: 27175940
  41. Besides controlling cyclin/CDK kinase activity, p27 also regulates cytoskeletal dynamics, cell motility and cell invasion. Following processing by caspases, p27 fails to bind to RhoA and to inhibit its activation, and thereby abolishes the ability of p27 to stimulate cell migration and invasion PMID: 26829051
  42. p27 is a key target of MDM4 oncogenic activity in breast cancer (BC) with mutant p53. PMID: 28097652
  43. a mechanism of transcriptional regulation mediated by p27, Pax5, and PCAF PMID: 28158851
  44. Our study has confirmed that altered expressions of the p16 and p27 proteins might be useful biomarkers in the progression of laryngeal squamous cell carcinomas PMID: 28476808
  45. This study to analyses cytoplasmic localization of p27 in OSCC and correlates it with prognosis. Cytoplasmic localization is associated with poor prognosis in OSCC with lymph node metastasis. PMID: 26750594
  46. Results show that AMPKa2 regulates cellular proliferation in bladder cancer through p27 and that AMPK-mediated control of p27 is SKP2 dependent. PMID: 27638620
  47. High KIP1 expression is associated with Metastasis in Osteosarcoma. PMID: 27197201
  48. The lack of somatic CDKN1B mutations in our samples points to a rare involvement in parathyroid adenomas, despite the frequent loss of nuclear p27 expression. MEN1 biallelic inactivation seems to be directly related to down-regulation of p27 expression through the inhibition of CDKN1B gene transcription. PMID: 27038812
  49. our results provide strong evidence that CD244 co-operates with c-Kit to regulate leukemogenesis through SHP-2/p27 signaling. PMID: 28126968
  50. The anti-proliferative effect of silibinin on LX-2 human stellate cells is via the inhibition of the expressions of various cell cycle targets including TP53, p27, Akt and sirtuins. PMID: 28119262
  51. Our study was the first to screen for CDKN1B mutations, which have been shown to cause MEN4, in a Chinese population. Nevertheless, no CDKN1B mutation was detected in the present study, which supports the rarity of CDKN1B gene mutation. PMID: 27846313
  52. ACTB, CDKN1B, GAPDH, GRB2, RHOA and SDCBP are potent reference genes in neuroendocrine tumors of the lung. PMID: 27802291
  53. p27 protein expression is controlled by SIRT1 in contact inhibition. PMID: 27514448
  54. Higher expression of Skp2 correlates with lower expression of p27kip1 in gastric cancer tissues. PMID: 27572672
  55. These data reinforce the P27 underexpression in pituitary adenomas and provide further evidence of the post-translational machinery involvement. PMID: 27824399
  56. the interplay between CRM-1 and p27Kip1 may provide potentially potent biomarkers and functional targets for the development of future cholangiocarcinoma treatments. PMID: 27279267
  57. Data indicate that high expression of p16 and p53 and low expression of p27 were related with the distinguishing of papillary thyroid carcinoma (PTC) from benign lesions. PMID: 26446458
  58. TRIP6 overexpression promotes tumor proliferation and reverses cell adhesion-mediated drug resistance via regulating nuclear p27(Kip1) expression in non-Hodgkin's lymphoma PMID: 26298725
  59. cytoplasmic p27, via ERK-activated Bcl-2 expression may predict an unfavorable response to cisplatin-based chemotherapy and poor outcomes in non-small cell lung cancer. PMID: 26482622
  60. High KIP1 expression is associated with metastasis occurrence in Prostate Cancer. PMID: 27268646
  61. Combining the expression level of different cyclins may be useful in determining the prognosis in endometrial cancer. Unfortunately, it remains unclear whether high p27 expression is a poor or a favorable prognostic factor PMID: 27919969
  62. LMO2 was associated with increased levels of cytosolic p27(Kip1) protein. PMID: 26383528
  63. High levels of miR-148a inhibits CDKN1B activity and promotes the proliferation of multiple myeloma cells. PMID: 27842905
  64. miR-222 confers the resistance of breast cancer cells to adriamycin through suppression of p27(kip1) expression. PMID: 27282281
  65. Data indicate that SUMOylation is a novel regulatory mechanism that modulates p27(Kip1) function in response to TGFbeta stimulation. PMID: 26450989
  66. CacyBP/SIP nuclear translocation contributes to the proliferation of gastric cancer cells, and CacyBP/SIP exerts this effect, at least in part, by stimulating ubiquitin-mediated degradation of p27Kip1. PMID: 27099442
  67. the protein status of p27(Kip1) is mainly determined by p38-MAPK, whereas the role of SKP2 is less significant in the doxoroubicin-treated A549 cells. PMID: 20716117
  68. Results show that association of IRF4 with p27 may provide mechanistic links between IRF4 and cell proliferation inhibition of gastric cancer cells. PMID: 26781452
  69. Through functional studies, we uncover cryptic sequence features within the p27 intrinsically disordered regions ( IDR) that influence the efficiency of T187 phosphorylation. PMID: 27140628
  70. p27 overrides Myc's suppression of senescence independent of Ser-62 and induces Myc degradation independent of Cdk2 by binding Myc via its C-terminus. Further, high expression of active p27 protein significantly correlated with low Myc protein level in human breast cancer. PMID: 26701207
  71. Data show that KDM5B is frequently up-regulated in hepatocellular carcinoma tissues and cell lines and correlated with poor prognosis. Its depletion leads to highly impaired clonogenesis and cell cycle arrest through up-regulation of p15 and p27. PMID: 26911146
  72. Somatic Mutations and Genetic Heterogeneity at the CDKN1B Locus is associated with Small Intestinal Neuroendocrine Tumors PMID: 25586243
  73. Results show that histological subtyping of pituitary tumor correlated with SSTR2, E cadherin and p27kip protein levels and these may serve as useful biomarkers for prognosis. PMID: 26391562
  74. results implicate Eya4/Six1 regulates normal cardiac function via p27/casein kinase-2alpha/histone deacetylase 2 and indicate that mutations within this transcriptional complex and signaling cascade lead to the development of cardiomyopathy. PMID: 26499333
  75. Data show that cytoplasmic cyclin-dependent kinase inhibitor p27 is preferentially observed in primary non-small cell lung cancer (NSCLC) with activated proto-oncogene proteins c-akt1 and predicts poor survival. PMID: 26053093
  76. HDACIs increase p27(Kip1) by hampering its nuclear ubiquitination/degradation PMID: 26682002
  77. These findings provide evidence that miR-24 has a tumor suppressor role in prostate cancer and also targets p27 and p16 in prostate cancer cells PMID: 26847530
  78. Cx43-Hsc70 interaction regulates cell cycle G1/S progression through a novel mechanism by which Cx43-Hsc70 interaction prevents the nuclear accumulation of p27 through controlling the nuclear translocation of cyclin D1-CDK4-p27 complex. PMID: 26481195
  79. These results define a novel mechanism by which PKM2 regulates glioma cell growth, and also define a novel set of potential therapeutic targets along the PKM2-HuR-p27 pathway PMID: 26874904
  80. measurements comprising the receptors EGFR, ERBB3 (HER3), and the cyclin-dependent kinase inhibitor p27 (CDKN1B) was found to accurately predict dependence on PI3K/AKT vs. MAPK/ERK signaling axes in HER2+ cancers PMID: 27035903
  81. Inhibition of cell cycle entry and progression in a variety of cancers may be achieved with therapies designed to correct p27Kip1 localization and/or block its degradation. (Review) PMID: 26279144
  82. Suppression of p27Kip1 following increased miR-150 expression promotes the development and progression of prostate cancer. PMID: 26636522
  83. eIF3a improves ovarian cancer patients' response to cisplatin-based chemotherapy by down regulating XPC and p27(Kip1). PMID: 26213845
  84. These findings provide further evidence that histatin 3 may be involved in the regulation of cell proliferation, particularly during G1/S transition, via the ubiquitin-proteasome system of p27(Kip1) and HSC70. PMID: 26775844
  85. MARCKS knockdown arrested VSMC cell cycle by decreasing KIS expression. Decreased KIS expression resulted in nuclear trapping of p27kip1 in VSMCs. PMID: 26528715
  86. Low expression of KIP1 is associated with Advanced Ovarian Cancer. PMID: 26320455
  87. Data show that Rheb/p27 axis induces autophagy-dependent cancer cell survival under stress conditions. PMID: 25594310
  88. COP1 directly interacts with p27 through a VP motif on p27 and functions as an E3 ligase of p27 to accelerate the ubiquitin-mediated degradation of p27. COP1-p27 axis deregulation is involved in tumorigenesis. PMID: 26254224
  89. The data demonstrates that PHD3 can drive cell cycle entry at the G1/S transition through decreasing the half-life of p27 that occurs by attenuating p27S10 phosphorylation. PMID: 26223520
  90. This supports the notion that synchronous activation of Galectin-3 and HBME-1 occurs in thyroid malignancy and is highly specific for malignancy. PMID: 25221953
  91. Data identified p27Kip1 as a direct target of miR-24-3p, and its protein level was negatively regulated by miR-24-3p. This implies that miR-24-3p/p27Kip1 axis has potential as a therapeutic target for breast cancer. PMID: 26044523
  92. P27 expression mediates drug resistance of gastric cancer cells towards an antineoplastic agent MLN4924; its inhibition reverses the resistance. PMID: 26313918
  93. A novel lncRNA, LUADT1, promotes lung adenocarcinoma proliferation via the epigenetic suppression of p27. PMID: 26291312
  94. Data show that heterogeneous nuclear ribonucleoprotein A0 (hnRNPA0) drives chemo-resistance of p53-mutant tumor cells via p27Kip1/Gadd45alpha mRNAs. PMID: 26602816
  95. CDKN1B V109G polymorphism seems to play a role in the development of aggressive tumours in Multiple endocrine neoplasia type 1. PMID: 25824098
  96. the Skp2mediated degradation of p27kip1 was important in the proliferation of tumor cells. The present study, therefore, provided a molecular reference for the treatment of liver cancer. PMID: 25572801
  97. Our data suggest that p27(Kip1) down-regulation in cervical cancer cells is primarily regulated through PI3K/AKT-mediated proteasomal degradation PMID: 25821107
  98. DLL4/Notch1 and BMP9 interdependent signaling induces endothelial cell quiescence via P27KIP1/thrombospondin pathway. PMID: 26471266
  99. Review and meta-analysis data suggest that carriage of a TG genotype at CDKN1B rs2066827 polymorphism may be associated with decreased susceptibility to cancer. PMID: 26579796
  100. Further studies showed that the expression of p27 was dependent on ERK1/2 activation, and p27 itself could halt cell cycle by inhibiting the activation of CDKs PMID: 25894374

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Involvement in disease Multiple endocrine neoplasia 4 (MEN4)
Subcellular Location Nucleus, Cytoplasm, Endosome
Protein Families CDI family
Tissue Specificity Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
Database Links

HGNC: 1785

OMIM: 600778

KEGG: hsa:1027

STRING: 9606.ENSP00000228872

UniGene: Hs.238990

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