The production of the IFNAR1 recombinant monoclonal antibody involves the use of DNA recombinant technology and in vitro genetic manipulation. Initially, animals are immunized with a synthesized peptide derived from human IFNAR1, and B cells are isolated from them for further screening. Positive B cells are selected, and individual clones are identified. The light and heavy chains of the IFNAR1 antibody are then amplified through PCR and inserted into a plasmid vector. This recombinant vector is transfected into a host cell line to facilitate antibody expression. The IFNAR1 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. This antibody exhibits specificity towards human IFNAR1 protein and is highly suitable for use in ELISA.
The IFNAR1 protein is a receptor protein involved in the cellular response to interferons, which are proteins that play a critical role in the immune response to viral infections and other pathogens. When interferons bind to the IFNAR1 receptor on the surface of a cell, a signal is initiated that activates a signaling pathway known as the JAK-STAT pathway. This leads to the expression of a variety of genes that play a role in antiviral defense and other immune responses. In addition to its role in the immune response, IFNAR1 has also been implicated in other cellular processes, including cell proliferation and differentiation.
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