Target Names
N (Nucleoprotein)
Alternative Names
N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
Species Reactivity
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Immunogen
Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)
CSB-EP3325GMY
Immunogen Species
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Isotype
Mouse scFv fusion with human IgG1 Fc
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Tested Applications
ELISA
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description
The production of the N recombinant monoclonal antibody involves a systematic process to ensure its high quality and specific binding properties. The journey begins by isolating B cells from the spleen of an immunized animal, where the recombinant human SARS-CoV-2 N protein (1-419aa) is used as the immunogen. RNA is then extracted from these B cells and converted into cDNA through reverse transcription. The N antibody genes are amplified using specific primers designed for the antibody constant regions and cloned into an expression vector. The human IgG1 Fc is inserted into the vector, downstream of the N antibody gene and the HRP is also incorporated into the vector. This recombinant vector is introduced into host cells through transfection, allowing for the production of the N recombinant monoclonal antibody. After an appropriate incubation period, the antibody is collected from the cell culture supernatant and purified using affinity chromatography to ensure a highly pure form. The purified N recombinant monoclonal antibody is characterized using ELISA analysis to validate its specificity and functionality in detecting human SARS-CoV-2 N protein. Through this comprehensive production process, a reliable and effective N recombinant monoclonal antibody is generated, making it an invaluable tool for various SARS-CoV-2-associated research.
