Recombinant Human Dipeptidyl peptidase 4(DPP4)

Code CSB-CF007139HU
Size Pls inquire
Source in vitro E.coli expression system
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Product Details


The cDNA fragment encoding full-length human dipeptidyl peptidase 4(DPP4) was expressed in vitro E.coli expression system. CUSABIO offers a custom tag service. DPP4 is a serine protease that regulates postprandial islet hormone secretion and glycemia by rapidly inactivating the incretin peptides, glucagon-like peptide-1, and glucose-dependent insulinotropic polypeptide. It also exerts noncatalytic effects through its interaction with ligands such as caveolin-1, extracellular matrix, CXCR4, and ADA. DPP4-ADA interaction stimulates T-cell action. DPP4 has also been confirmed to physically interact with caveolin-1 on antigen-presenting cells, inducing aggregation and phosphorylation, which activates NF-κB.

Target Names DPP4
Uniprot No. P27487
Alternative Names DPP4; ADCP2; CD26; Dipeptidyl peptidase 4; ADABP; Adenosine deaminase complexing protein 2; ADCP-2; Dipeptidyl peptidase IV; DPP IV; T-cell activation antigen CD26; TP103; CD antigen CD26
Species Homo sapiens (Human)
Expression Region 1-766
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones such as brain natriuretic peptide 32. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.; (Microbial infection) Acts as a receptor for human coronavirus MERS-CoV-2.
Gene References into Functions
  1. Our results showed 14% inferior plasma DPP-4 activity in adolescent idiopathic scoliosis (AIS) patients when compared to healthy controls . Similarly, osteoblasts derived from AIS subjects had lower DPP-4 gene and protein expression than controls by 90.5% and 57.1% respectively (P < 0.009). DPP-4 expression was regulated in a different manner in osteoblasts isolated from AIS participants compared to controls. PMID: 28600546
  2. Here we review the recent advances in identifying the cellular sources of both circulating and membrane-bound DPP4 important for cleavage of the incretin hormones and regulation of glucose and lipoprotein metabolism. PMID: 29412815
  3. DPP4 activity when compared to healthy controls. Analysis of Dpp4-Liv-Tg mice revealed elevated systemic DPP4 activity and diminished active GLP-1 levels. PMID: 29031724
  4. DPP4 was not different in this group of polycystic ovarian syndrome patients. However, a relationship between DPP4 and markers of insulin resistance were found. PMID: 29680519
  5. The analysis of their molecular properties allowed the design of a multiple linear regression model for activity prediction. Their docking analysis allowed visualization of the interactions between the pharmacophore regions and hDDP-IV. PMID: 28027711
  6. The level of myocardial CD26 expression might be a predictive marker of prognosis in patients with heart failure. PMID: 30369565
  7. Our study showed that complement was excessively activated in MERS-CoV-infected hDPP4-Tg mice through observations of increased concentrations of the C5a and C5b-9 complement activation products in sera and lung tissues, respectively. PMID: 29691378
  8. API was demonstrated to inhibit the migration/invasion of NSCLC cells harboring different EGFR statuses via suppressing the Snail/Slug-mediated EMT..CD26 may be a useful biomarker for predicting NSCLC progression. API effectively suppressed lung cancer progression by targeting the CD26-Akt-Snail/Slug signaling pathway PMID: 30134935
  9. hepatocyte DPP4 promotes visceral adipose tissue inflammation and insulin resistance in obesity, and targeting this pathway may have metabolic benefits that are distinct from those observed with oral DPP4 inhibitors PMID: 29562231
  10. this study shows that CD26 expression is down-regulated on CD8(+) T cells in patients with Hashimoto's thyroiditis PMID: 29175506
  11. Several isoforms of DPP-IV and FAP are present in glioblastoma tissue. The absence of alkaline isoforms of both enzymes in glioma cell lines however suggests that isoforms from other, most likely stromal, cell types contribute to the overall pattern seen in glioblastoma tissues. PMID: 28452380
  12. Data suggest that dipeptidyl peptidase 4 (DPPIV) gene polymorphism influences disease susceptibility and acute pancreatitis (AP) severity. PMID: 28902797
  13. High levels of DPP4a are independently associated with an increased rate of no-reflow events. PMID: 28000723
  14. DPP-4 activity and GLP-1total levels were higher in patients with microvascular complications associated with T2DM. Contrary to expectations, no negative correlation was seen between GLP-1 and DDP-4 levels. This result suggests the possible inefficacy of DDP-4 activity as a marker to predict in vivo degradation of endogenous GLP-1. PMID: 29061224
  15. The study showed that DPP-4 inhibitor use does not modify the risk of bone fracture compared with placebo or other anti-diabetic medications in patients with type 2 diabetes. PMID: 27384445
  16. A close connection between SerpinB3 and DPPPIV has been identified, but further studies are required to better understand the mechanism by which these proteins communicate and exert metabolic effects in hepatocellular carcinoma. PMID: 29524519
  17. There is a U-shaped association of serum DPP-IV with mortality in chronic heart failure patients. PMID: 28318663
  18. Levels of DPPIV enzyme activity in sera of patients with psoriatic pruritus were significantly increased compared to those of healthy controls. PMID: 28365081
  19. These findings implicate that DPP4 plays a role in the aggressiveness of UCs, and can serve as a novel prognostic marker and therapeutic target. PMID: 27936466
  20. Data suggest that up-regulation of serum DPP4 activity is associated with severity of diabetic nephropathy/renal insufficiency/albuminuria in subjects with type 1 diabetes. PMID: 27625045
  21. DPPIV overexpression altered cell morphology and stimulated cell proliferation, invasion and tumorigenesis in endometrial carcinoma cells. PMID: 28060721
  22. MERS-CoV S protein binds to DPP4 to suppress macrophage activation via induction of IRAK-M, PPARgamma and the immunosuppressive cytokine IL-10. PMID: 28118607
  23. DPP4a may be associated with future major adverse cardiac or cerebrovascular events in diabetic STEMI patients. PMID: 28587613
  24. DPP4 inhibitors have been demonstrated to be beneficial in the improvement of endothelial homeostasis. PMID: 28440488
  25. As for 18Lin(-), CD34(-) HSCs are characterized by low expression of the tetraspanin CD9, which promotes homing, and high expression of the peptidase CD26, which inhibits homing. PMID: 28687990
  26. suggests that DPP4 T-molecules have modified binding and functions compared with their FL counterparts and may serve regulatory roles in normal and malignant hematopoiesis PMID: 28344320
  27. Analysis of both the structures and inhibitory activities suggests that the binding of the trifluorophenyl moiety in the S1 pocket and the piperazine-2-one moiety have hydrophobic interactions with Phe357 in the S2 extensive subsite, and that the multiple hydrogen bonds made by the (R)-beta-amine group in the S2 pocket and the contacts made by the (R)-tert-butyl group with Arg125 contribute to the high potency observed... PMID: 29061303
  28. the selective expression of DPP4 on the surface of senescent cells enables their preferential elimination. PMID: 28877934
  29. Study shows that increased DPP4 activities are independently associated with mild cognitive impairment in elderly patients with type 2 diabetes. PMID: 27371673
  30. A common variant, i.e., single nucleotide polymorphism rs6741949, in the DPP4 gene interacts with body adiposity and negatively affects glucose-stimulated GLP-1 levels, insulin secretion, and glucose tolerance. PMID: 28750074
  31. CUX-1 overexpression enhanced TNF- production on sCD26/LPS stimulation, while CUX-1 depletion had no effect. Neither CUX-1 overexpression nor CUX-1 depletion had an effect on IL-6 stimulation. These results are discussed in the context of a model that describes the mechanisms by which stimulation of monocytic cells by sCD26 and LPS leads to elevation of TNF- and IL-6 expression. PMID: 23805228
  32. Increased plasma DPP-4 activity may correlate with aneurysmal development. CD26 on monocytes plays a critical role in cell differentiation, possibly mediated by extracellular signal-regulated protein kinase 1/2-p21 axis signaling pathways and cytoskeletal proteins reassembly. PMID: 27887857
  33. Plasma DPP4 activity is significantly associated with NAFLD. The underlying mechanisms may be partly attributed to the interactions between insulin resistance, oxidative stress, inflammation, and DPP4. PMID: 28637594
  34. CD26+ fibroblasts possess proliferation advantage in comparison to CD26- fibroblasts, and the advantage caused expansion of CD26 positive fibroblast population promotes keloid progression. PMID: 28454879
  35. MFSD2B, CCL20 and STAT1, or STARD7 and ZNF512 genes may be risk or protect factors in prognosis of ADC; HTR2B, DPP4, and TGFBRAP1 genes may be risk factors in prognosis of SQC. PMID: 27301951
  36. Increased DPP4 expression is associated with cellular invasion and more aggressive disease in papillary thyroid cancer. DPP4 silencing or treatment with DPP4 inhibitors significantly suppressed colony formation, cell migration, and invasion. PMID: 28575350
  37. Cultured human skin fibroblasts displayed significantly higher levels of CD26 than gingival fibroblasts. This was associated with an increased expression of profibrotic genes and transforming growth factor-beta signaling in skin fibroblasts. PMID: 28641076
  38. Suggest DPP-4 inhibition has a neutral effect on cardiovascular risk in type 2 diabetics without a history of heart failure or renal insufficiency. PMID: 28145158
  39. To clarify whether DPP4 alters its general or partial structure according to the inhibitor used and whether DPP4 has a common rule for inhibitor binding, this study comprehensively analyzed X-ray cocrystal structures of DPP-4 and its inhibitor. All the main and side chains in the inhibitor binding area were minimally altered, except for a few side chains, despite binding to inhibitors of various shapes. PMID: 27491540
  40. DPP-4-dependent insulin like growth factor-1 inhibition in valvular interstitial cells contributes to aortic calcification. PMID: 28179397
  41. Data suggest that, in obesity, serum levels of active GLP1 are down-regulated and serum levels of soluble DPP4 are up-regulated; DPP4 levels correlate negatively with active GLP-1 levels but are positively associated with insulin resistance; thus, DPP4 may be biomarker for insulin resistance. This study was conducted in Malaysia. (GLP1 = glucagon-like peptide 1; DPP4 = dipeptidyl peptidase 4) PMID: 28288852
  42. YS110 is a humanised IgG1 monoclonal antibody with high affinity to the CD26 antigen. YS110 demonstrated preclinical anti-tumour effects without significant side effects PMID: 28291776
  43. Gain of CD26 expression on the malignant T-cells in relapsed erythrodermic leukemic mycosis fungoides PMID: 28083948
  44. like AprA, DPPIV is a chemorepellent for, and inhibits the proliferation of, D. discoideum cells, and that AprA binds some DPPIV binding partners such as fibronectin. PMID: 28028841
  45. CD26 expression should be incorporated in B-CLL patients risk assessment along with well known prognostic factors, since it seems to have a relationship with the tumor mass and influences TTT. PMID: 27376546
  46. Serum CD26 concentration might be an independent prognostic indicator in patients with esophageal squamous cell carcinoma . It might be also useful to detect recurrent tumor in postoperative patients. PMID: 27664490
  47. The trimeric form receptor-binding domain of MERS-CoV spike protein protected hDPP4 transgenic mice from lethal MERS-CoV challenge. PMID: 27750111
  48. Data suggest that dipeptidyl-peptidase 4 [DPP-4) induces a selective pathway of insulin resistance with reduced glycogen storage, enhanced glucose output and increased lipid accumulation in the liver. PMID: 28213130
  49. Analysis of human liver biopsy specimens revealed a correlation of DPP4 expression and DNA methylation to stages of hepatosteatosis and nonalcoholic steatohepatitis. PMID: 27999105
  50. The study suggests that a genetic interaction between DPP4 and CCR6 is involved in RA susceptibility PMID: 27587881

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Subcellular Location [Dipeptidyl peptidase 4 soluble form]: Secreted.; Cell membrane; Single-pass type II membrane protein. Apical cell membrane; Single-pass type II membrane protein. Cell projection, invadopodium membrane; Single-pass type II membrane protein. Cell projection, lamellipodium membrane; Single-pass type II membrane protein. Cell junction. Membrane raft.
Protein Families Peptidase S9B family, DPPIV subfamily
Tissue Specificity Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed
Database Links

HGNC: 3009

OMIM: 102720

KEGG: hsa:1803

STRING: 9606.ENSP00000353731

UniGene: Hs.368912

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