Recombinant Rat Tyrosine-protein phosphatase non-receptor type substrate 1 (Sirpa)

Code CSB-CF021334RA
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Source in vitro E.coli expression system
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Product Details

Target Names
Uniprot No.
Alternative Names
Sirpa; Bit; Mfr; Ptpns1; Shps1; Sirp; Tyrosine-protein phosphatase non-receptor type substrate 1; SHP substrate 1; SHPS-1; Brain Ig-like molecule with tyrosine-based activation motifs; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; Macrophage membrane protein MFP150; Signal-regulatory protein alpha-1; Sirp-alpha-1; CD antigen CD172a
Rattus norvegicus (Rat)
Expression Region
Target Protein Sequence
Protein Length
Full Length of Mature Protein
Tag Info
The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

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Target Background

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. May play a role in the release of nitric oxide by macrophages.
Gene References into Functions
  1. In the glomeruli of CNS patients carrying mutations in NPHS1, where SD formation is disrupted, the expression of SIRPalpha as well as Neph1 and nephrin was significantly decreased, indicating that SIRPalpha is closely associated with the nephrin complex PMID: 22747997
  2. Data suggest that hyperglycemia induces increase in association of SHPS-1 with IAP (integrin-associated protein); this appears to be a generalized response of endothelial cells that contributes to endothelial cell dysfunction. PMID: 22193512
  3. Data demonstrate that BIT/SHPS-1 promotes the antiapoptotic effect of BDNF on the low potassium-induced cell death of cerebellar granule neuronss. PMID: 21553247
  4. Data show that myelin down-regulates its own phagocytosis through CD47-SIRPalpha interactions. PMID: 21401967
  5. CD47-SIRP-alpha interaction may be functionally important in cell-cell communication in the diseased glomerulus. PMID: 20554646
  6. BIT is involved in the entrainment of circadian rhythms through the function of NMDARs and non-NMDARs. PMID: 12763253
  7. Gp93 was identified as the Ig superfamily member SIRPalpha (also known as SHPS-1, BIT or P84); in rat brain, gp93/SIRPalpha heterogeneity is the result of differential glycosylation (plus phosphorylation), rather than sequence heterogeneity PMID: 12807424
  8. Tyrosine phosphorylation level of BIT in the retina is higher in the light period than in the dark period. PMID: 14741368
  9. may play a role in the termination of hepatic proliferation during liver regeneration induced by physiological stress or pathological states, such as PH, drugs, toxins PMID: 15047126
  10. BIT is involved in the mechanism of neuronal responses to cold exposure in the hypothalamus PMID: 15158458
  11. the level of the SHPS-1 mRNA increases in rat skeletal muscle after denervation. Glycosylation of SHPS-1 is N-linked in a muscle-specific manner, and that this is altered upon innervation or denervation. PMID: 15858173
  12. SIRPalpha ligation induces macrophage NO production through the cooperative action of JAK/STAT and PI3-K/Rac1/NOX/H(2)O(2) signaling pathways and may function as an activating receptor PMID: 16055727
  13. SIRP alpha and SHP-1 potentially mediate the inhibitory effect of elastic laminae on monocyte adhesion. PMID: 16159885
  14. These results suggest that SIRPalpha functions in the modulation of axonal growth by extracellular matrix molecules, such as laminin. PMID: 16371655
  15. crystallization and X-ray diffraction data collection of the ligand-binding domain of SHPS-1 PMID: 16511298
  16. inhibition by SHPS-1 of phagocytosis in macrophages is mediated, at least in part, in a manner independent of the transinteraction between CD47 and SHPS-1 PMID: 16920950

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Tissue Specificity
Highly expressed in brain, spleen, lung, liver and kidney. Detected at lower levels in heart. Highly expressed in alveolar and peritoneal macrophages, and at lower levels in dendritic cells.
Database Links
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