Name: IGSF8 (GV20-0251 Biosimilar) Recombinant Monoclonal Antibody
Brand: CUSABIO
SKU: CSB-RA856887MB1HU

Product Details

Uniprot No.

Q969P0

Target Names

IGSF8

Alternative Names

GV20 0251 research-grade biosimilar; XBH25 research-grade biosimilar ;IGSF8 antibody; CD81P3 antibody; EWI2 antibody; KCT4 antibody; Immunoglobulin superfamily member 8 antibody; IgSF8 antibody; CD81 partner 3 antibody; Glu-Trp-Ile EWI motif-containing protein 2 antibody; EWI-2 antibody; Keratinocytes-associated transmembrane protein 4 antibody; KCT-4 antibody; LIR-D1 antibody; Prostaglandin regulatory-like protein antibody; PGRL antibody; CD antigen CD316 antibody

Species Reactivity

Human

Immunogen

Recombinant Human IGSF8 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

0.01M PBS，pH7.4

Form

Liquid

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Notes

Validation Status
Application-specific performance (e.g., in flow cytometry, ELISA, IHC or other assay formats) has not yet been experimentally verified by CUSABIO. Users are advised to determine the optimal working conditions empirically in their own assay systems.
Guaranteed Quality
① Antibody purity > 95% tested by SDS-PAGE.
② Endotoxin level < 0.1EU/ug tested by LAL method.

Lead Time

3-4 weeks

Description

This recombinant monoclonal antibody is developed as a research-grade biosimilar to the reference antibody GV20-0251, specifically targeting IGSF8 (Immunoglobulin Superfamily Member 8), also known as EWI-2 or CD316. IGSF8 is a transmembrane glycoprotein that functions as a key regulator of cell adhesion, migration, and signal transduction by associating with tetraspanins such as CD9 and CD81. This protein plays important roles in various physiological processes including sperm-egg fusion, immune cell function, and cancer cell metastasis. IGSF8 expression has been implicated in several pathological conditions, particularly in tumor progression and metastasis, where it influences cancer cell motility and invasiveness across multiple malignancies.

The reference antibody GV20-0251 has been utilized in research investigating IGSF8's molecular interactions and cellular functions. This biosimilar provides researchers with a reliable tool for exploring IGSF8-mediated pathways in cell biology, immunology, and oncology studies. The antibody enables investigation of IGSF8's role in tetraspanin-enriched microdomains and its contribution to cellular processes relevant to cancer research and reproductive biology.

Usage

It is a non-therapeutic biosimilar antibody, owning the same variable region from the corresponding approved therapeutic antibody. In conclusion, it is a research-grade biosimilar antibody and expressed in mammalian cell, which can be directly used as positive controls in drug discovery or used for rapid verification of the biological functions of target protein.

Target Background

Function

May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.

Gene References into Functions

EWI-2 negatively regulates TGF-beta signaling and its downstream events including cytostasis (in vitro and in vivo), EMT-like changes, cell migration, CD271-dependent invasion, and lung metastasis (in vivo). PMID: 25656846
Authors demonstrated that EWI-2wint promotes CD81 clustering and confinement in CD81-enriched areas. PMID: 23351194
The EWI-2/alpha-actinin complex is involved in regulation of the actin cytoskeleton at T cell immune and virological synapses, providing a link between membrane microdomains and the formation of polarized membrane structures involved in T cell recognition. PMID: 22689882
analysis of interacting regions of CD81 and two of its partners, EWI-2 and EWI-2wint, and their effect on hepatitis C virus infection PMID: 21343309
EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and inhibits the migration of prostate cancer cells. PMID: 12750295
EWI-2-dependent reorganization of alpha4beta1-CD81 complexes on the cell surface is responsible for EWI-2 effects on integrin-dependent morphology and motility functions PMID: 15070678
EWI proteins EWI-2 and EWI-F, alpha3beta1 and alpha6beta4 integrins, and protein palmitoylation have contrasting effects on cell surface CD9 organization PMID: 16537545
EWI-2 and EWI-F link the tetraspanin web to the actin cytoskeleton through their direct association with ezrin-radixin-moesin proteins PMID: 16690612
Important functions of recently activated dendritic cells are thus critically modulated by the newly discovered HSPA8-EWI-2 interaction. PMID: 17785435
CD81 partner EWI-2wint inhibits hepatitis C virus entry [EWI-2wint] PMID: 18382656
EWI-2 causes a substantial molecular reorganization of multiple molecules known to affect proliferation and/or invasion of astrocytes and/or glioblastomas. PMID: 19107234

Hide All

Subcellular Location

Cell membrane; Single-pass membrane protein.

Tissue Specificity

Expressed in brain, kidney, testis, liver and placenta with moderate expression in all other tissues. Detected on a majority of B-cells, T-cells, and natural killer cells but not on monocytes, polynuclear cells and platelets.

Database Links

HGNC: 17813

OMIM: 606644

KEGG: hsa:93185

STRING: 9606.ENSP00000316664

UniGene: Hs.332012

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Code	CSB-RA856887MB1HU
Size	US$9799
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IGSF8 (GV20-0251 Biosimilar) Recombinant Monoclonal Antibody

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Target Background