High-Sensitivity ELISA Kits for Low-Abundance Detection

Why Choose CUSABIO High-Sensitivity ELISA Kits?

Ultimate Sensitivity

Detection limit as low as 0.002 pg/mL, with some products reaching femtogram-level lower limits, >100 times improvement over traditional ELISA. Provides precise assurance for your low-abundance sample detection.

Coverage of Hot Research Areas

230+ HS series ELISA kits, covering 15+ species and 200+ targets, comprehensively covering hot areas such as immunology, neuroscience, cardiovascular, metabolism, and oncology, supporting your various research needs.

8 Major Quality Control Validations

Products undergo multi-dimensional validation including linearity, recovery, precision, specificity, stability, etc., suitable for various sample types such as serum, plasma, cell supernatant, tissue homogenate, with low CV values ensuring result reproducibility.

Full-Process Support

Ready-to-use kits, complete operation instructions, delivery within 3-5 business days, and full technical support team response, allowing you to have no worries during experimental design and operation.

CUSABIO ELISA kits have been cited in 32,000+ SCI papers

Trusted by global researchers

HS Series Product Catalog

Species:

Target:

Target	Product Name	Product Name	Sensitivity	Code

Selected Citations

VEGF-C-driven lymphatic drainage enables immunosurveillance of brain tumours. Nature, 2020. IF=50.5

This study demonstrates that enhancing VEGF-C expression in a mouse brain tumor model promotes meningeal lymphatic drainage, enhances CD8⁺ T cell priming and tumor infiltration in the deep cervical lymph nodes, thereby improving brain tumor immune surveillance and potentiating the efficacy of immune checkpoint therapy. In this study, the CUSABIO Mouse VEGF-C ELISA Kit (CSB-E07361m) was used to detect VEGF-C levels in mouse cerebrospinal fluid (CSF), meninges, brain tissue, and serum, to verify the expression/distribution of VEGF-C following AAV-VEGF-C or VEGFC mRNA treatment.

In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation. Cell, 2017. IF=45.5

This study developed and validated a CRISPR/Cas9-mediated in vivo target gene activation system that can activate endogenous genes via trans-epigenetic modulation, producing measurable ameliorative effects in mouse disease models of diabetes, muscular dystrophy, and acute kidney injury. In this study, the CUSABIO Mouse Klotho ELISA Kit (CSB-E14362m) was used to detect the levels of secreted Klotho protein in mouse serum, helping to verify that the AAV-delivered gene activation system indeed increased Klotho protein expression, thereby providing experimental evidence for its functional improvement in the acute kidney injury model.

Bone-targeting engineered small extracellular vesicles carrying anti-miR-6359-CGGGAGC prevent valproic acid-induced bone loss. Signal transduction and targeted therapy, 2024. IF=40.8

This study found that valproic acid (VPA) promotes osteoclast formation and bone resorption through miR-6359/SIRT3/ROS/MAPK-related mechanisms, leading to reduced bone mass and bone mineral density in patients and mice. The researchers constructed bone-targeting engineered small extracellular vesicles carrying anti-miR-6359-CGGGAGC to alleviate VPA-induced bone loss. In this study, the CUSABIO Mouse CTX-II ELISA Kit (CSB-E16211m) was used to detect CTX-II levels in mouse serum as an indicator of bone resorption/bone metabolism changes, helping to evaluate the bone loss caused by VPA and the ameliorative effects following intervention with the engineered small extracellular vesicles.

Gut microbial DL-endopeptidase alleviates Crohn's disease via the NOD2 pathway. Cell host & microbe, 2022. IF=31.316

This study found that gut microbiota-derived DL-endopeptidase increases the levels of NOD2 ligands in the intestine, and that this enzyme is reduced in patients with Crohn's disease. Supplementation with DL-endopeptidase or related NOD2 ligands in a mouse model of colitis alleviates intestinal inflammation via the NOD2 pathway. In this study, the CUSABIO Mouse IL-6 ELISA Kit (CSB-E04639m) was used to detect IL-6 levels in mouse experiments as an indicator of the inflammatory response, helping to assess whether the severity of colitis inflammation was ameliorated following DL-endopeptidase intervention.

Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice. Cell host & microbe, 2023. IF=31.316

This study found that APAP-induced liver injury is accompanied by alterations in the gut microbiota, among which Lactobacillus vaginalis can release daidzein from the diet via its β-galactosidase and alleviate APAP-induced hepatotoxicity by inhibiting Fdps-associated ferroptosis in hepatocytes. In this study, the CUSABIO Mouse MCP-3 ELISA Kit (CSB-E07426m) was used to measure MCP-3 concentrations in the blood of mice following APAP exposure. The results showed that blood MCP-3 levels in L. vaginalis -treated mice were lower than those in the control group, supporting its ameliorative effect on APAP-induced inflammation/liver injury.

Gut microbial co-metabolite 2-methylbutyrylcarnitine exacerbates thrombosis via binding to and activating integrin a2b1. Cell Metabolism, 2024. IF=30.9

This study found that the gut microbial metabolite 2-methylbutyrylcarnitine (2MBC) is elevated in patients with COVID-19 and major adverse cardiovascular events, and can enhance platelet reactivity and thrombosis risk by binding to and activating platelet integrin α2β1. In this study, the CUSABIO Rat TXB2 ELISA Kit (CSB-E08047r) was used to measure plasma TXB2 levels to assess platelet activation/thrombosis-related responses; and the CUSABIO Mouse IL-6 ELISA Kit (CSB-E04639m) was used to detect mouse plasma IL-6 as an inflammation-related indicator to help evaluate the physiological responses following 2MBC treatment.

Q&A

Q：What is a high-sensitivity ELISA kit? +

A：A high-sensitivity ELISA kit is designed to quantify low-abundance target molecules in biological samples. Compared with conventional ELISA kits, high-sensitivity ELISA kits generally offer a lower detection limit and better performance in the low-concentration range, making them suitable for detecting cytokines, hormones, neuropeptides, growth factors, and other low-level biomarkers in research samples.

Q：When should I choose a high-sensitivity ELISA kit? +

A：A high-sensitivity ELISA kit is recommended when the target analyte is expected to be present at a very low concentration, when the sample volume is limited, or when a conventional ELISA kit may not provide sufficient sensitivity. Typical applications include low-level cytokine detection, early inflammatory response studies, hormone or neuropeptide measurement, and serum or plasma analysis in animal models.

Q：Does a lower detection limit always mean a better kit? +

A：Not always. A lower detection limit indicates that the kit can detect lower concentrations of the target analyte, but kit selection should also consider the sample type, expected concentration range, assay range, linearity, recovery, precision, and specificity. For samples with relatively high target levels, a suitable detection range can be just as important as sensitivity.

Q：What sample types can be used with CUSABIO high-sensitivity ELISA kits? +

A：CUSABIO high-sensitivity ELISA kits are suitable for common research sample types such as serum, plasma, cell culture supernatant, and tissue homogenates. Supported sample types may vary by product, so researchers are encouraged to check the product datasheet or contact technical support before starting the experiment.

Q：How do I choose the right high-sensitivity ELISA kit? +

A：To select the right kit, first confirm the target analyte, species, sample type, and expected concentration range. For low-abundance targets, pay close attention to the sensitivity and the low-end assay range. For complex sample matrices, specificity, recovery, and linearity data should also be considered. If you are unsure, CUSABIO technical support can help recommend a suitable product based on your research needs.

Q：What should I pay attention to when measuring low-abundance samples? +

A：Low-abundance detection requires careful sample handling and consistent operation. Avoid repeated freeze-thaw cycles, mix samples thoroughly, follow the protocol for incubation, washing, and color development, and run replicates whenever possible. A preliminary test or dilution series is also recommended when the expected concentration range is unclear.

Q：Can a high-sensitivity ELISA kit be used for samples from different species? +

A：Each ELISA kit is developed for specific species. Because antibody recognition and antigen epitopes may differ between species, cross-species use is not recommended unless it has been validated. If your sample species is not listed in the product information, please contact technical support to evaluate potential cross-reactivity.

Q：What should I do if my sample concentration is outside the assay range? +

A：If the sample concentration is above the standard curve range, dilute the sample with the recommended diluent and repeat the assay, then multiply the result by the dilution factor. If the concentration is below the assay range, consider optimizing sample preparation, concentrating the sample if appropriate, or selecting a kit with higher sensitivity and a more suitable detection range.

Q：Does CUSABIO provide product selection and technical support? +

A：Yes. CUSABIO can help researchers select suitable high-sensitivity ELISA kits based on the target analyte, species, sample type, expected concentration range, and research purpose. Technical support is also available for sample preparation, assay operation, and result interpretation.