Alternative Names
BRICD6 ELISA Kit; BRICHOS domain containing 6 ELISA Kit; PSP C ELISA Kit; PSPC ELISA Kit; PSPC_HUMAN ELISA Kit; Pulmonary surfactant apoprotein 2 ELISA Kit; Pulmonary surfactant apoprotein PSP C ELISA Kit; pulmonary surfactant apoprotein-2 SP-C ELISA Kit; Pulmonary surfactant associated protein C ELISA Kit; Pulmonary surfactant associated proteolipid SPL pVal ELISA Kit; Pulmonary surfactant associated proteolipid SPL(Val) ELISA Kit; Pulmonary surfactant protein SP5 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit; SFTP 2 ELISA Kit; SFTP2 ELISA Kit; SFTPC ELISA Kit; SFTPC surfactant pulmonary associated protein C ELISA Kit; SMDP2 ELISA Kit; SP 5 ELISA Kit; SP C ELISA Kit; SP-C ELISA Kit; SP5 ELISA Kit; SPC ELISA Kit; Surfactant associated protein pulmonary 2 ELISA Kit; Surfactant protein c ELISA Kit; Surfactant proteolipid SPL-pVal ELISA Kit; Surfactant pulmonary associated protein C ELISA Kit
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.41 ng/ml - 300 ng/ml
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% |
|
Three samples of known concentration were tested twenty times on one plate to assess. |
Inter-assay Precision (Precision between assays): CV%<10% |
|
Three samples of known concentration were tested in twenty assays to assess. |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
Sample |
Serum(n=4) |
1:1 |
Average % |
92 |
|
Range % |
89-99 |
1:2 |
Average % |
97 |
|
Range % |
91-101 |
1:4 |
Average % |
98 |
|
Range % |
92-105 |
1:8 |
Average % |
96 |
|
Range % |
86-100 |
Recovery
The recovery of human SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
Sample Type |
Average % Recovery |
Range |
Serum (n=5) |
96 |
89-99 |
EDTA plasma (n=4) |
98 |
90-105 |
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
ng/ml |
OD1 |
OD2 |
Average |
Corrected |
300 |
1.931 |
1.872 |
1.902 |
1.741 |
100 |
1.512 |
1.444 |
1.478 |
1.317 |
33.3 |
0.958 |
0.904 |
0.931 |
0.770 |
11.1 |
0.462 |
0.486 |
0.474 |
0.313 |
3.7 |
0.288 |
0.306 |
0.297 |
0.136 |
1.23 |
0.265 |
0.252 |
0.259 |
0.098 |
0.41 |
0.229 |
0.210 |
0.220 |
0.059 |
0 |
0.168 |
0.153 |
0.161 |
|
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx