Name: Fhit Antibody
Brand: CUSABIO
SKU: CSB-PA530819HA01MO

Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Fhit Polyclonal antibody

Uniprot No.

O89106

Target Names

Fhit

Alternative Names

FhitBis(5'-adenosyl)-triphosphatase antibody; EC 3.6.1.29 antibody; AP3A hydrolase antibody; AP3Aase antibody; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase antibody; Dinucleosidetriphosphatase antibody; Fragile histidine triad protein antibody

Raised in

Rabbit

Species Reactivity

Mouse

Immunogen

Recombinant Mouse Bis(5\\\'-adenosyl)-triphosphatase protein (2-150AA)

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

The Fhit Antibody (Product code: CSB-PA530819HA01MO) is Non-conjugated. For Fhit Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA530819HB01MO	Fhit Antibody, HRP conjugated	ELISA
FITC	CSB-PA530819HC01MO	Fhit Antibody, FITC conjugated
Biotin	CSB-PA530819HD01MO	Fhit Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Related Products

Target Background

Function

Possesses dinucleoside triphosphate hydrolase activity. Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Exhibits adenylylsulfatase activity, hydrolyzing adenosine 5'-phosphosulfate to yield AMP and sulfate. Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Exhibits adenylylsulfate-ammonia adenylyltransferase, catalyzing the ammonolysis of adenosine 5'-phosphosulfate resulting in the formation of adenosine 5'-phosphoramidate. Also catalyzes the ammonolysis of adenosine 5-phosphorofluoridate and diadenosine triphosphate. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity. Functions as tumor suppressor.

Gene References into Functions

Fhit loss and subsequent thymidine kinase 1 inactivation, combined with selective pressures, leads to neoplasia-associated alterations in genes and gene expression patterns in vitro and in vivo PMID: 27513973
Fhit-deficiency mutation signature also resembles a C>T and T>C mutation signature reported for human papillary kidney cancers and a similar signature recently reported for esophageal and bladder cancers, cancers that are frequently Fhit deficient. PMID: 26782170
Fhit deficiency-induced global genome instability promotes mutation and clonal expansion PMID: 24244712
Fhit delocalizes annexin A4 from plasma membrane to cytosol and sensitizes lung cancer cells to paclitaxel. PMID: 24223161
FHIT gene is a "caretaker gene" necessary for maintenance of genome stability. PMID: 23929738
Loss of Fhit expression contributes to cell transformation. PMID: 23102829
Defects in Fhit expression may promote MHC-I down-regulation in cancer cells and allow escape from immunosurveillance. PMID: 22451343
Human and mouse orthologous genes, FHIT and Fhit, are more highly conserved through evolution than PTPRG/Ptprg and yet contain more sequence elements that are exquisitely sensitive to genomic rearrangements PMID: 14630947
Fhit has a role in bladder cancer development PMID: 15569992
UV-induced alterations of the FHIT and WWOX fragile site gene expression are involved at least partially in the checkpoint function of DNA damage PMID: 15798093
Data suggest that heterozygosity for FHIT affects susceptibility of mice to spontaneous alopecia areata and benzo[a]pyrene-induced preneoplastic lesions of the uterus and does not alter responsiveness to budesonide and N-acetyl-L-cysteine. PMID: 16672365
Nit1 and Fhit share tumor suppressor signaling pathways, while localization of the NIT1 gene at a stable chromosome site explains the paucity of gene alterations and in frequent loss of expression of the NIT1 gene in human malignancies. PMID: 16864578
It is likely that the FHIT transgene is more tightly silenced in female transgenic mice, leading to a lack of protection from tumor induction PMID: 18000371
tumor-like microdeletions in FHIT/FRA3B are induced by replication stress PMID: 18162546
reduced oxidative stress, coupled with efficient but not error-free DNA damage repair, allows unscheduled long-term survival of genotoxin-exposed Fhit-deficient hematopoietic stem cells carrying deleterious mutations PMID: 18483248
the extent of tumor susceptibility due to Nit1 and Fhit deficiency is additive PMID: 19479888

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Subcellular Location

Cytoplasm. Nucleus. Mitochondrion.

Tissue Specificity

Expressed in heart, brain, lung and skeletal muscle. Particularly strong expression in liver, testis and kidney, where it is confined to the tubular epithelium.

Database Links

KEGG: mmu:14198

STRING: 10090.ENSMUSP00000123874

UniGene: Mm.397619