Recombinant Mouse C-C motif chemokine 21c protein (Ccl21c) is produced in an E. coli expression system, covering the full length of the mature protein from amino acids 24 to 133. This tag-free protein achieves purity levels exceeding 97% as determined by SDS-PAGE and maintains endotoxin levels below 1.0 EU/μg as measured by the LAL method. The protein demonstrates full biological activity, effectively promoting chemotaxis in murine T-lymphocytes at concentrations ranging from 10-100 ng/ml.
C-C motif chemokine 21c protein appears to play a critical role in immune response regulation. It guides lymphocyte movement to lymphoid tissues—a key function in adaptive immunity. Ccl21c is likely essential for research focused on understanding cellular migration and immune cell trafficking, which may make it valuable for immunology studies and related fields.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. T-lymphocyte Chemotaxis Assays
This recombinant Ccl21c protein works well as a positive control or standard in chemotaxis assays studying T-lymphocyte migration patterns. The confirmed biological activity in the 10-100 ng/ml concentration range makes it suitable for dose-response studies examining T-cell directional migration. Scientists can apply this protein to investigate the molecular mechanisms underlying lymphocyte trafficking and to validate experimental chemotaxis systems. High purity levels (>97%) and low endotoxin content ensure reliable and reproducible results in cell-based migration assays.
2. Chemokine Receptor Binding Studies
Biologically active Ccl21c protein serves as a ligand in receptor binding assays to characterize chemokine receptor interactions. Scientists can apply this protein in competitive binding experiments or saturation binding studies to determine receptor affinity and specificity. The tag-free nature eliminates potential interference from fusion tags that might affect receptor binding kinetics. These studies may contribute to understanding chemokine-receptor signaling pathways and receptor pharmacology in mouse models.
3. Antibody Development and Validation
This high-purity recombinant Ccl21c protein works as an immunogen for generating specific antibodies against mouse Ccl21c or as a standard for antibody validation experiments. The full-length mature protein sequence (24-133aa) provides the complete antigenic profile for comprehensive antibody screening. Scientists can apply this protein in ELISA-based assays, Western blot validation, or immunoprecipitation experiments to characterize antibody specificity and sensitivity. Low endotoxin content makes it suitable for immunization protocols in antibody production workflows.
4. Protein-Protein Interaction Studies
Recombinant Ccl21c protein can be applied in biochemical assays to identify and characterize protein interactions involved in chemokine signaling networks. Scientists may use this protein in pull-down assays, surface plasmon resonance experiments, or co-immunoprecipitation studies to map molecular interactions. High purity and biological activity suggest that observed interactions reflect physiologically relevant binding events. These studies can help elucidate the molecular basis of chemokine function and identify novel regulatory proteins in lymphocyte signaling pathways.
5. Preclinical Research Models
Biologically active Ccl21c protein can be used in preclinical mouse studies to investigate immune cell recruitment and inflammatory responses. Scientists can administer the protein in controlled concentrations to study its effects on lymphocyte distribution and tissue infiltration patterns. The confirmed activity range provides a starting point for dose optimization in various experimental models. Such studies may contribute to understanding the role of Ccl21c in immune system function and inflammatory disease mechanisms in mouse models.
