Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ABI4 Polyclonal antibody

Uniprot No.

A0MES8

Target Names

ABI4

Alternative Names

ABI4 antibody; ERF052 antibody; GIN6 antibody; ISI3 antibody; SAN5 antibody; SIS5 antibody; SUN6 antibody; At2g40220 antibody; T7M7.16Ethylene-responsive transcription factor ABI4 antibody; ERF ABI4 antibody; Protein ABSCISIC ACID INSENSITIVE 4 antibody; Protein GLUCOSE INSENSITIVE 6 antibody; Protein IMPAIRED SUCROSE INDUCTION 3 antibody; Protein SALOBRENO 5 antibody; Protein SUCROSE UNCOUPLED 6 antibody; Protein SUGAR INSENSITIVE 5 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ABI4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcription regulator that probably binds to the GCC-box pathogenesis-related promoter element. Binds also to the S-box (5'-CACTTCCA-3') photosynthesis-associated nuclear genes-related (PhANGs-related) promoter element, and thus acts as a transcription inhibitor. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways. May have a function in the deetiolation process. Confers sensitivity to abscisic acid (ABA), and regulates the ABA signaling pathway during seed germination, upon nitrate-mediated lateral root inhibition, in hexokinase-dependent sugar responses (including feed-back regulation of photosynthesis and mobilization of storage lipid during germination), and in response to osmotic stress mediated by NaCl, KCl or mannitol. Plays a role in sucrose sensing or signaling, especially at low fluence far red light. Also involved in plant response to glucose treatment, especially at low concentration and in young seedlings. Required for the trehalose-mediated root inhibition and starch accumulation in cotyledons, probably by inhibiting starch breakdown. However, seems to not be involved in sugar-mediated senescence. Required for the ABA-dependent beta-amino-butyric acid (BABA) signaling pathway. BABA primes ABA synthesis and promotes resistance to drought and salt, and leads to a prime callose accumulation that confers resistance against necrotrophic pathogens such as A.brassicicola and P.cucumerina. Seems to be involved in resistance to S.sclerotiorum probably by regulating the ABA-mediated stomatal closure apparently by antagonistic interaction with oxalate. Negative regulator of low water potential-induced Pro accumulation whose effect is decreased by high levels of sugar.

Gene References into Functions

Compared to the wild-type (Col-0) Arabidopsis thaliana plants, the abi4 mutant demonstrated increased susceptibility to two-spotted spider mite, reflected as enhanced female fecundity and greater frequency of mite leaf damage after trypan blue staining. PMID: 29210003
The photorespiratory phenotype of cat2-2 mutants did not depend on the SHR functional interactor SCARECROW and the sugar signaling component ABSCISIC ACID INSENSITIVE4, despite the requirement for exogenous sucrose for cell death attenuation in cat2-2 shr-6 double mutants. PMID: 27432873
ABI4 plays a key role in abscisic acid and cytokinin signaling during eed germination and cotyledon greening.ABI4 suppresses the transcription of ARR6, ARR7 and ARR15 PMID: 27711992
Molecular analysis showed that BPCs bind to the ABI4 promoter and repress ABI4 transcription in roots. PMID: 28138058
ABA-INSENSITIVE 4 (ABI4) is a central factor in abscisic acid (ABA) and gibberellins (GAs) homeostasis and antagonism in post-germination stages. PMID: 26708041
ABSCISIC ACID-INSENSITIVE 4 (ABI4), a key component in the abscisic acid signalling pathway, negatively regulates floral transition by directly promoting FLOWERING LOCUS C (FLC) transcription. ABI4-overexpressing plants had delayed floral transition. PMID: 26507894
Disruption of RETARDED ROOT GROWTH-LIKE (RRL) decreased while overexpression increased ABA sensitivity during seed germination and seedling growth. ABI4 is a downstream regulatory factor in this RRL-mediated ABA signalling. PMID: 26163700
MYB96-ABI4 module regulates lipid mobilization specifically in the embryo to ensure proper seed germination under suboptimal conditions. PMID: 25869652
Chromatin immunoprecipitation-qPCR and transient expression analysis showed that ABI4 directly binds to the GSQ11/ANAC060 promoter to activate transcription. PMID: 24625790
The degradation of ABI4 protein through the PEST motif is mediated by the 26S proteasome in response to changes in the sugar levels. PMID: 24046063
The abi4, cbfA and cbp mutants showed weaker drought-tolerance after a herbicide norflurazon treatment, which indicated the physiological role of these key transcription factors. PMID: 23832569
ABI4 downregulates expression of the sodium transporter HKT1;1 in Arabidopsis roots and affects salt tolerance. PMID: 23240817
this study suggests that ABI4 is a key factor that regulates primary seed dormancy by mediating the balance between abscisic acid and gibberellins biogenesis. PMID: 23818868
suppressor mutation causing the phenotype of soa1 occurred in the ABI4 (ABA insensitive 4) gene PMID: 23196831
Low ascorbate triggers ABA- and jasmonate-dependent signaling pathways that together regulate growth through ABI4. PMID: 21926335
ABI4 promoter activity during seedling development was followed, focusing on the roots. PMID: 21448003
The study examined ABI4 protein accumulation indirectly, using transgenic lines expressing fusions to beta-glucuronidase. Inclusion of ABI4 coding sequence reduced the ratio of activity to transcript by ~150-fold when controlled by the ABI4 promoter. PMID: 21504878
Data suggest that ABI4 mediates abscisic acid and cytokinin inhibition of lateral root formation via reduction of polar auxin transport and that the resulting decrease in root auxin leads to a reduction in lateral root development. PMID: 21097710
Double-mutant analysis showed that ABA-insensitive 4 (ABI4) is epistatic to AtLPP2 but ABA-insensitive 3 (ABI3) is not. PMID: 15960620
Impaired responses to light, sugars, and abscisic acid in ABI mutants of A. thaliana. PMID: 16098105
ABI4 functions as a repressor of lipid breakdown. PMID: 16844907
Study defines abscisic acid(ABA)-induced resistance to L. maculans as a subset of the ABA responses regulated via ABI-1 and ABI4. PMID: 17427804
The abi4 phenotype of Arabidopsis expressing tomator ASR1 is the result of competition between the foreign ASR1 and the endogenous ABI4 on specific promoter DNA sequences. PMID: 18363631
ABI4 binds directly to the promoter region of ABI5 and SBE2.2, and its own promoter, and activates expression in vivo through a CE1-like element. PMID: 19392689
ABI4 plays a central role in mediating mitochondrial retrograde signals to induce the expression of AOX1a. PMID: 19482916

Hide All

Subcellular Location

Nucleus.

Protein Families

AP2/ERF transcription factor family, ERF subfamily

Tissue Specificity

In seeds, mostly in embryo, and seedlings, especially in vascular tissues. Confined to the hypocotyl, cotyledons, the root cap, and the root quiescent center.

Database Links

KEGG: ath:AT2G40220

STRING: 3702.AT2G40220.1

UniGene: At.50594

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ABI4 Antibody

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