Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ADO1 Polyclonal antibody

Uniprot No.

Q94BT6

Target Names

ADO1

Alternative Names

ADO1 antibody; FKL2 antibody; LKP1 antibody; ZTL antibody; At5g57360 antibody; MSF19.2Adagio protein 1 antibody; Clock-associated PAS protein ZTL antibody; F-box only protein 2b antibody; FBX2b antibody; Flavin-binding kelch repeat F-box protein 1-like protein 2 antibody; FKF1-like protein 2 antibody; LOV kelch protein 1 antibody; Protein ZEITLUPE antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ADO1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Component of an E3 ubiquitin ligase complex that plays a central role in blue light-dependent circadian cycles. Acts as a blue light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO1) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including the transition to flowering time, hypocotyl elongation, cotyledons and leaf movement rhythms. APRR1/TOC1 and APRR5, but not 'GIGANTEA', are proteolytic substrates of this ubiquitin ligase complex. Blue light enhances cooperative stabilization of 'GIGANTEA' and ADO1/ZTL, leading to amplification and sharpening of the expression profile of APRR1/TOC1. ADO1/ZTL interacts with ADO3, preventing the interaction of ADO3 with CDF1.

Gene References into Functions

the potential roles of ZTL in thermal responses and stability of the circadian clock in plants, are reported. PMID: 29172942
Data suggest that ZEITLUPE (ZTL) E3 ubiquitin ligase-mediated protein quality control contributes to the thermal stability of the circadian clock. PMID: 29061867
The results reveal that ZTL complexes with circadian clock proteins independent of light, but dictates enhanced protein degradation in the dark. PMID: 28244872
These data suggest the involvement of auxin and auxin-inducible genes in ZTL-mediated hypocotyl elongation. PMID: 26237185
functions as a positive regulator in warmth-induced hypocotyl elongation under light PMID: 26039487
The fast photocycle allows detection of the day-night transition facilitating circadian timing. ZTL kinetics reflect an evolutionary adaptation of the ZTL/FKF1/LKP2 family to function in distinct aspects of blue-light signaling. PMID: 24033190
As the absence of either GI or ZTL compromises clock function and diminishes the protein abundance of the other, our results highlight how their reciprocal co-stabilization is essential for robust circadian oscillations. PMID: 24004949
Mutations in the N terminus of ZTL interfere with binding to EBI. ZTL opposes the effects of EBI on circadian clock components during the day. PMID: 21300918
The SCFZTL complex is characterized in detail. PMID: 15447654
11 new ztl alleles encompassing mutations in each of the ZTL protein domains are described. PMID: 16428597
GIGANTEA is essential to establish and sustain oscillations of ZTL by a direct protein-protein interaction PMID: 17704763
TOC1/PRR3 phosphorylation-dependent interaction may protect TOC1 from ZTL-mediated degradation, resulting in an enhanced amplitude of TOC1 cycling PMID: 18562312

Hide All

Subcellular Location

Nucleus. Cytoplasm. Note=Nuclear after 9 hours of illumination (afternoon of long days). Cytoplasmic when plant have been subsequently grown 16 hours in light and 5 hours in dark (early morning of long days).

Protein Families

ADAGIO family

Tissue Specificity

Ubiquitously expressed with higher levels in cotyledons and leaves.

Database Links

KEGG: ath:AT5G57360

STRING: 3702.AT5G57360.2

UniGene: At.21022

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ADO1 Antibody

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