Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ASHH2 Polyclonal antibody

Uniprot No.

Q2LAE1

Target Names

ASHH2

Alternative Names

ASHH2 antibody; EFS antibody; LAZ2 antibody; SDG8 antibody; SET8 antibody; At1g77300 antibody; T14N5.15Histone-lysine N-methyltransferase ASHH2 antibody; EC 2.1.1.- antibody; ASH1 homolog 2 antibody; H3-K4-HMTase antibody; Histone H3-K36 methyltransferase 8 antibody; H3-K36-HMTase 8 antibody; Protein EARLY FLOWERING IN SHORT DAYS antibody; Protein LAZARUS 2 antibody; Protein SET DOMAIN GROUP 8 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ASHH2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Histone methyltransferase involved in di and tri-methylation of 'Lys-36' of histone H3 (H3K36me2 and H3K36me3). Binds to H3 already mono- or di-methylated on 'Lys-4'(H3K4me1 or H3K4me2), but not to H3K4me3. H3K4me and H3K36me represent specific tags for epigenetic transcriptional activation. Regulates positively FLC transcription to prevent early flowering transition. Required for flowering transition in response to vernalization and for the maintenance of FLC expression in late embryos, but dispensable for the initial reactivation in early embryos during reprogramming. Seems also to modulate several traits including floral organ size, root size and dormancy. Promotes apical dominance. Directly involved in the tri-methylation of 'Lys-36' of histone H3 (H3K36me3) at LAZ5 chromatin to maintain a transcriptionally active state of LAZ5, a TIR-NB-LRR protein involved in innate immunity. Required for brassinosteroid (BR)-induced gene expression and histone H3 trimethylation on 'Lys-36' (H3K36me3) in BR-regulated genes.

Gene References into Functions

findings provide first structural insights into the molecular basis for specific recognition of monomethylated H3K4 by the H3K4me1 reader protein SDG8 from Arabidopsis. PMID: 29496997
both maternal and paternal EFS alleles are responsible for the seed size regulation PMID: 29372509
SDG8 plays an essential role in mediating BR-regulated gene expression PMID: 24838002
provide evidence that ASHH2 is acting on H3K4me-marked genes, allowing for ASHH2-dependent H3K36 tri-methylation, which contributes to sustained expression of tissue-specific and developmentally regulated genes. PMID: 21522130
SDG8 is required for basal and R protein-mediated pathogen resistance in Arabidopsis, revealing the importance of chromatin remodeling as a key process in plant innate immunity. PMID: 20949080
SDG8 plays crucial roles in plant defense against necrotrophic fungal pathogens through histone H3K36me3-mediated activation of a subset of genes within the jasmonate/ethylene pathway. PMID: 20810545
These results suggest that SDG8 regulates shoot branching via controlling the methylation states of its target genes. PMID: 18602372
The CCR1 gene encodes a histone methyltransferase that methylates histone H3 and downregulates CRTISO. PMID: 19174535

Hide All

Subcellular Location

Nucleus. Chromosome, centromere.

Protein Families

Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, SET2 subfamily

Tissue Specificity

Ubiquitous, with higher levels in young tissues, including shoot and root apex. Expressed in ovules, tapetum layer and microspores.

Database Links

KEGG: ath:AT1G77300

STRING: 3702.AT1G77300.1

UniGene: At.34505

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ASHH2 Antibody

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