Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) BIK1 Polyclonal antibody

Uniprot No.

O48814

Target Names

BIK1

Alternative Names

BIK1 antibody; At2g39660 antibody; F12L6.32 antibody; F17A14.3Serine/threonine-protein kinase BIK1 antibody; EC 2.7.11.1 antibody; Protein BOTRYTIS-INDUCED KINASE 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana BIK1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Plays a central role in immune responses. Required to activate the resistance responses to necrotrophic pathogens, including the regulation of defense hormone expression (e.g. jasmonic acid (JA) and salicylic acid (SA)). Phosphorylates FLS2 and BAK1. Involved in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling, including calcium signaling, and defense responses downstream of FLS2; upon PAMP recognition, first phosphorylated by FLS2 prior to being monoubiquitinated by ATL44/RHA3A and ATL45/RHA3B at the plasma membrane, then internalized dynamically into endocytic compartments together with FLS2. Acts additively with PBL1 in PTI defenses. Acts as positive regulator of the PAMP flg22-induced increase of cytosolic calcium. Binds directly and phosphorylates the NADPH oxidase RBOHD at specific sites in a calcium-independent manner to enhance reactive oxygen species (ROS) generation upon flg22 perception. ROS production in response to flg22 controls stomatal movement and restriction of bacterial entry into leaf tissues. Seems not required for flg22-induced MAPK activation. Required for Pep1-induced defenses. Pep1 is an endogenous elicitor that potentiates PAMP-inducible plant responses. In association with PEPR1, acts downstream of the canonical ethylene signaling cascade to regulate ethylene responses. Involved in ethylene signaling. Destabilizes EIN3, the key transcription activator in ethylene signaling, and represses EIN3-dependent transcription. Acts as a negative regulator in brassinosteroid (BR) signaling. Functions in BR signaling by direct interaction with the BR receptor BRI1 cytosolic kinase domain.; (Microbial infection) Xanthomonas campestris effector AvrAC/XopAC-mediated uridylylation prevents activation by phosphorylation at the same residues, thus affecting immune responses and reducing defense responses toward X.campestris, mediating avrAC/XopAC virulence functions.

Gene References into Functions

These findings demonstrate the mechanistic basis of signal transduction from EFR to phytohormones, mediated through a EFR-BIK1-WRKY axis. PMID: 29649442
Purification, crystallization and X-ray diffraction analysis of BIK1 has been reported. PMID: 27710938
Results demonstrate that BIK1 is a Pi starvation-responsive gene that functions as a negative regulator of Pi homeostasis in Arabidopsis. PMID: 27389008
These data suggest that BIK1 cooperates with BAK1 to regulate constitutive immunity and cell death. PMID: 28225199
Data show that Xanthomonas oryzae pv. oryzae (Xoo) PXO99A XopR protein interacts with receptor-like cytoplasmic kinase BIK1 at the plasma membrane. PMID: 27520828
Upon pathogen-associated molecular patterns perception, PP2C38 is phosphorylated on serine 77 and dissociates from the FLS2/EFR-BIK1 complexes, enabling full BIK1 activation. PMID: 27494702
autophosphorylated BIK1 in the plasma membrane negatively regulates EIN3-dependent gene expression. PMID: 26021844
CPK28 contributes to the PAMP-induced Ca(2+) burst, supporting its role as a negative regulator of BIK1. PMID: 26039480
CPK28 facilitates BIK1 turnover and negatively regulates BIK1-mediated immune responses triggered by several pathogen-associated molecular patterns PMID: 25525792
required for microbe- or damage-associated molecular pattern-induced calcium signaling PMID: 25522736
study first mapped the phosphorylation patterns of BAK1 associated with four RLK partners (BRI1, FLS2, EFR and BIK1 PMID: 24953020
These findings highlight a direct role of the FLS2-BIK1 complex in the regulation of RbohD-mediated ROS production and stomatal defense. PMID: 24629339
Direct regulation of the NADPH oxidase RBOHD by the PRR-associated kinase BIK1 regulates plant antibacterial immunity. PMID: 24630626
The essential function of tyrosine kinase activity of BIK1 in plants echoes the function of nonreceptor tyrosine kinases that transduce receptor tyrosine kinase signaling via dimerization and phosphorylation in metazoans. PMID: 24532660
BIK1 mediates inverse functions in plant immunity and development via dynamic association with specific receptor complexes and differential phosphorylation events. PMID: 23818580
BIK1 interacts with PEPR1 and PEPR2 to amplify ethylene-induced immune responses PMID: 23431184
The critical role of BIK1 in plant immune responses hinges upon phosphorylation, its function in ethylene signaling, and complex interactions with other immune response regulators. PMID: 21862710
Xanthomonas campestris pathovar campestris type III effector AvrAC enhances virulence and inhibits plant immunity by specifically targeting Arabidopsis BIK1 and RIPK, two receptor-like cytoplasmic kinases known to mediate immune signalling. PMID: 22504181
Results identify the receptor-like cytoplasmic kinase BIK1 that is rapidly phosphorylated upon flagellin perception, depending on both FLS2 and BAK1. PMID: 20018686
BOTRYTIS-INDUCED KINASE1 (BIK1) modulates the signaling of cellular factors required for defense responses to pathogen infection and normal root hair growth, linking defense response regulation with that of growth and development.[BIK1] PMID: 16339855

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Subcellular Location

Cell membrane; Lipid-anchor. Endosome membrane; Lipid-anchor. Nucleus.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Database Links

KEGG: ath:AT2G39660

STRING: 3702.AT2G39660.1

UniGene: At.21254

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BIK1 Antibody

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