Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Caf1-55 Polyclonal antibody

Uniprot No.

Q24572

Target Names

Caf1-55

Alternative Names

Caf1 antibody; CG4236Probable histone-binding protein Caf1 antibody; Chromatin assembly factor 1 p55 subunit antibody; CAF-1 p55 subunit antibody; Nucleosome-remodeling factor 55 kDa subunit antibody; NURF-55 antibody; dCAF-1 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Caf1-55 protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the nucleosome remodeling and deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the nucleosome remodeling factor (NURF) complex, which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin; and the polycomb group (PcG) repressor complex ESC-E(Z), which promotes repression of homeotic genes during development. Also required for transcriptional repression of E2F target genes by E2f2 and Rbf or Rbf2.

Gene References into Functions

CCR4 and CAF1, the two deadenylases in the CCR4-NOT complex, can remove 3' terminal non-A residues in an exonucleolytic manner. PMID: 27484313
CAF1-p180 and CAF1-p75 subunits mediate assembly of two different forms of chromatin. PMID: 23810557
CAF-1 promotes Notch signaling through epigenetic control of target gene expression during Drosophila development. PMID: 23942516
these studies suggest that p55 is not crucial for PRC2-mediated gene silencing in vivo, and the vital function of p55 is probably not dependent on its interaction with histone H4. PMID: 22241697
Chromatin-modifying complex component Nurf55/p55 associates with histones H3 and H4 and polycomb repressive complex 2 subunit Su(z)12 through partially overlapping binding sites. PMID: 21550984
analysis of Caf1 mutant tissue suggests that Caf1 plays important roles in cell survival and segment identity, and loss of Caf1 is associated with a reduction in the Polycomb Repressive Complex 2 (PRC2)-specific histone methylation mark H3K27me3 PMID: 21490066
spreading of heterochromatin is compromised in flies that have reduced CAF-1 p180. Furthermore, reduced CAF-1 p180 causes a defect in the dynamics of heterochromatic markers in early Drosophila embryos. PMID: 20663913
Results establish that dCAF-1-p180 is an essential gene for Drosophila development and further underscore the importance of dCAF-1 in regulating gene expression and DNA repair in vivo. PMID: 17916346
Structural basis of histone H4 recognition by p55. PMID: 18443147
larval endocycling cells lacking CAF-1 large subunit exhibit normal dynamics of progression through endocycles, although accumulating defects, such as perturbation of nucleosomal organisation, reduction of DNA replication and accumulation of DNA damage PMID: 19066929

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Subcellular Location

Nucleus.

Protein Families

WD repeat RBAP46/RBAP48/MSI1 family

Database Links

KEGG: dme:Dmel_CG4236

STRING: 7227.FBpp0082511

UniGene: Dm.1657

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Caf1-55 Antibody

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