CDC14 Antibody

1. Cdc14 plays very specific, and often different, roles in counteracting Cdk phosphorylation in all species. PMID: 28663385
2. Activation of Cdc14 at anaphase onset coincides with its release from the nucleolus into the nucleus and subsequently into the cytoplasm. This study describes a microscopy method, originally developed in the laboratory of Frederick Cross (Lu and Cross, Cell 141:268-279, 2010), that allows quantifying Cdc14 release in live cells using the open source software FIJI. PMID: 27826856
3. The phosphatase Cdc14 has a pivotal function in the mitotic exit of Saccharomyces cerevisiae. The activity of Cdc14 can be used as a readout to assay functional interactions of different components of the mitotic exit signaling pathways. PMID: 27826859
4. Recently, several lines of evidences have demonstrated that the mitotic phosphatase Cdc14 is required to ensure condensin loading onto chromosomes. Here is described an easy and reliable protocol to analyze Cdc14-dependent condensin loading onto specific genomic DNA regions by using a chromatin immunoprecipitation (ChIP) technique. PMID: 27826868
5. Together, these results show that Cdc14 promotes spindle stability and DNA double-strand break-spindle pole body tethering during DNA repair, and imply that metaphase spindle maintenance is a critical feature of the repair process. PMID: 27852625
6. Dephosphorylation of Iqg1 by Cdc14 regulates cytokinesis in budding yeast PMID: 26085509
7. The transient loss of Cdc14 activity causes cells to go through a single mitotic catastrophe with irreversible consequences for the genome stability of the progeny. PMID: 25971663
8. The crystal structure of Kap121p-Cdc14p complex is determined. PMID: 26022122
9. Cin8 mutants lacking critical CDK phosphorylation sites suppress the requirement for Cdc14 and Cdc5 in anaphase spindle elongation. PMID: 25706236
10. Swe1 regulation by Cdc14 allows proper coordination of spindle elongation with mitotic progression. PMID: 25406317
11. Here we systematically analyzed the positions of dephosphorylation sites for Cdc14 in the budding yeast Saccharomyces cerevisiae. PMID: 24319056
12. Have refined the substrate specificity of budding yeast Cdc14 and, using this insight, identified the Holliday junction resolvase Yen1 as a DNA repair target of Cdc14. PMID: 24631283
13. Master cell-cycle regulators, cyclin-dependent kinase (Cdk) and Cdc14 phosphatase, control the actions of Yen1 during mitosis. PMID: 24631285
14. Data indicate that Oaf1p, Pip2p and Adr1p are required for the initial binding of Oaf3p before oleate induction. PMID: 23088601
15. Zds1 regulates PP2A(Cdc55) activity and Cdc14 activation during mitotic exit through its Zds_C motif. PMID: 22427694
16. Cdc14 localizes at the site of cell division prior to the onset of cytokinesis. PMID: 22454527
17. Chs2 endoplasmic reticulum export requires the direct reversal of the inhibitory Cdk1 phosphorylation sites by Cdc14 phosphatase PMID: 22072794
18. Inactivation of Cdc14 causes silencing defects at the intergenic spacer sequences of ribosomal genes during interphase and at Y' repeats in subtelomeric regions during mitosis. PMID: 22020438
19. CLB activities are antagonized by the CDC14 phosphatase in order to couple cell cycle progression with cytokinesis at mitotic exit PMID: 21784165
20. Computational modelling of mitotic exit in budding yeast: the role of separase and Cdc14 endocycles. PMID: 21288956
21. dephosphorylation of the Dsn1 kinetochore protein in metaphase requires Cdc14 PMID: 20923974
22. Cdc14 targets proteins involved in wide-ranging mitotic events. PMID: 21127052
23. inactivates the mitotic checkpoint by dephosphorylating Sli15(INCENP) PMID: 20619650
24. Data show that Cdc14 activation requires the polo-like kinase Cdc5 together with either Clb-cyclin-dependent kinase (Cdk) or the MEN kinase Dbf2. PMID: 20660629
25. Study finds that this Cdc14 release oscillator functions at constant and physiological cyclin-Cdk levels, and is therefore independent of the Cdk oscillator. PMID: 20403323
26. Cdk1 phosphorylates the Mob1 protein to inhibit the activity of Dbf2-Mob1 kinase that regulates Cdc14 phosphatase. PMID: 20123997
27. We established that Cdc14p activity released by the FEAR pathway was required for proper condensin-to-rDNA targeting in anaphase. PMID: 15190202
28. results show that the FEAR network acts in part by stimulating phosphorylation of Net1 by Clb-Cdk, which directly weakens the interaction of Cdc14 with Net1, allowing for Cdc14 activation and mitotic exit PMID: 15273393
29. Our results suggest a requirement for Crm1p-dependent nuclear export of Cdc14p in coordinating mitotic exit and cytokinesis in budding yeast. PMID: 15917648
30. Cdc14 is retained in the nucleolus to support a favorable kinase/phosphatase balance while cells are replicating their DNA, in addition to the established role of Cdc14 sequestration in coordinating nuclear segregation with mitotic exit. PMID: 17116692
31. Ime2-dependent phosphorylation of a subset of cell-cycle proteins limits the effects of Cdc14 in meiosis. PMID: 17349956
32. Review identifies Ase1 as a key Cdc14 substrate and elucidates its coordination of spindle midzone assembly with metaphase to anaphase transition. PMID: 18235228
33. the opposing actions of Cdc28 and Cdc14 are primary factors limiting Acm1 to the interval from G(1)/S to late mitosis and are capable of establishing APC-independent expression patterns similar to APC substrates PMID: 18287090
34. Tof2 collaborates with Cdc14 specifically in rDNA segregation, presumably by targeting Cdc14 phosphatase activity to the nucleolus during anaphase to support resolution and compaction of this repetitive and highly transcribed DNA locus PMID: 18595708
35. Cdc14 is activated by Zds1 and Zds2 at anaphase onset. PMID: 18762578
36. Data show that Utp7 plays essential roles in ribosome biogenesis, chromosome segregation, and cell cycle control, and that Utp7 associates with and regulates the localization of Sli15 and Cdc14. PMID: 18794331
37. These data support a model whereby Tof2 coordinates the biphasic release of Cdc14 during anaphase by restraining a population of Cdc14 in the nucleolus after activation of the Cdc14 early anaphase release (FEAR) network, for subsequent release by the MEN. PMID: 18923139
38. Data show that Cdc5 interacts with Cdc14, that that this association is mediated by Cdc5's Polo-box domain, and that the Cdc5-Cdc14 association is direct. PMID: 18927509
39. Cdc14, a protein phosphatase required for nucleolar segregation and mitotic exit, inhibits transcription of yeast ribosomal genes (rDNA) during anaphase PMID: 19158678
40. Study shows that a MEN component, protein kinase Dbf2-Mob1, promotes transfer of Cdc14 to the cytoplasm and consequent exit from mitosis by direct phosphorylation of Cdc14 on serine and threonine residues adjacent to a nuclear localization signal. PMID: 19221193
41. The spindle assembly checkpoint prevents separation of microtubule-organizing centers (spindle pole bodies [SPBs]) when spindle assembly is defective. PMID: 19339280
42. The authors found that Brn1 release in wild-type cells coincided with the release of Cdc14 phosphatase from the nucleolus and with mitotic CDK inactivation. PMID: 19387493
43. Results suggest that that Cdc5 promotes Cdc14 release primarily by stimulating the degradation of Swe1, the inhibitory kinase for mitotic Cdk. PMID: 19570916
44. Ubiquitination of histone H2B and methylations of histone H3 are required for the release of Cdc14 from nucleolar chromatin. PMID: 19662160
45. Data show that CDC14 mutations pose an overwhelming challenge to genome stability, both generating chromosome damage and undermining the checkpoint control mechanisms. PMID: 19666479

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KEGG: sce:YFR028C

STRING: 4932.YFR028C