Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) COF1 Polyclonal antibody

Uniprot No.

Q03048

Target Names

COF1

Alternative Names

COF1 antibody; YLL050C antibody; L0596Cofilin antibody; Actin-depolymerizing factor 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) COF1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. Binding to F-actin is regulated by tropomyosin. It is the major component of intranuclear and cytoplasmic actin rods. Required for accumulation of actin at the cell division site via depolymerizing actin at the cell ends. In association with myosin II has a role in the assembly of the contractile ring via severing actin filaments. Involved in the maintenance of the contractile ring once formed. In association with profilin and capping protein, has a role in the mitotic reorganization of the actin cytoskeleton. In effect, yeast cofilin increases the rate of actin polymerization by making new ends available for actin subunit addition. Such a protein complex is important for the polarized growth of yeast cells.

Gene References into Functions

Report structural basis for noncanonical substrate recognition of cofilin-1/LIMK1 to regulation actin cytoskeleton dynamics. PMID: 27153537
Data indicate that the combined activities of cofilin and Srv2/CAP lead to enhanced filament severing and support an emerging view that actin disassembly is controlled not by cofilin alone, but by a more complex set of factors working in concert. PMID: 23135996
Findings highlight the conserved role of cofilin and SPCA1/Pmr1 in sorting of the soluble secretory proteins at the trans-Golgi network/late Golgi membranes in eukaryotes. PMID: 22553351
Cofilin tunes the nucleotide state of actin filaments and severs at bare and decorated segment boundaries. PMID: 21530260
Cofilin binds AIP1 across the cleft between the two propeller domains, leaving the actin binding sites exposed and flanking the cofilin binding site. PMID: 16421248
actin depolymerizing factor/cofilins play an active role in establishing new interprotomer interfaces in F-actin that substitute for disrupted or weakened (as in ADP-actin) longitudinal contacts in filaments PMID: 16530787
role of pH in the severing by cofilin was examined using fluorescence microscopy PMID: 16847879
These results indicate that cofilin severs the actin filament by a mechanism of allosteric and cooperative destabilization. PMID: 17134718
ternary Aip1p/cofilin/actin filament complex model offers insights into how Aip1p may disturb intersubunit contacts within an actin filament PMID: 17483419
These results suggest that cofilin-mediated actin filament flux is required for the multiple steps of endocytic trafficking. PMID: 17875745
confirms the important role of the N-terminal segment of cofilin in interactions with G-actin PMID: 18258262
Results show that yeast cofilin 1 can efficiently depolymerize and sever yeast actin filaments decorated with either tropomyosin (Tpm)1 or mouse tropomyosins TM1 and TM4. PMID: 18982060
Data suggest a revised model for the mechanism by which Srv2 promotes actin turnover, in which coordinated activities of its N- and C-terminal halves catalyze sequential steps in recycling cofilin and actin monomers. PMID: 19201756

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Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix. Note=Throughout the cytoplasm (but not on the cytoplasmic cables) and major component of the cortical actin cytoskeleton.

Protein Families

Actin-binding proteins ADF family

Database Links

KEGG: sce:YLL050C

STRING: 4932.YLL050C

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COF1 Antibody

Product Details

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Target Background