Cyp6g1 Antibody

Code CSB-PA894648XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Cyp6g1 Polyclonal antibody
Uniprot No.
Target Names
Cyp6g1
Alternative Names
Cyp6g1 antibody; CYP6-like antibody; DDT-R antibody; Rst(2)DDT antibody; CG8453Cytochrome P450 6g1 antibody; EC 1.14.-.- antibody; CYPVIG1 antibody; Cyp6-like protein antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Cyp6g1 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Necessary and sufficient for resistance to insecticides DDT and imidacloprid. May be involved in the metabolism of insect hormones.
Gene References into Functions
  1. CYP6g1 role in the azinphos-methyl resistance PMID: 27317781
  2. a combination of gene duplication, regulatory changes and protein coding changes has taken place at the Cyp6g1 locus during evolution and this locus may play a role in providing resistance to different environmental toxins in different Drosophila species PMID: 24416303
  3. Recombinant D. melanogaster CYP6G1 exhibited p-nitroanisole O-dealkylation activity but was not active against eleven other typical P450 marker substrates. PMID: 23470655
  4. expression and tolerance to methylmercury during development PMID: 22699155
  5. Resistance to nicotine or related plant allelochemicals is one of the beneficial phenotypes conferred by the Accord insertion allele of CYP6G1. PMID: 22543020
  6. The induction response of the P450 gene Cyp6g1 to the xenobiotic phenobarbital requires the presence of both tissue specific enhancers and a distinct cis-regulatory element. PMID: 21807095
  7. The active site of Cyp6g1 can accommodate differently shaped substrates. PMID: 20583201
  8. Cyp6g1 locus is a major contributor to Dichloro-Diphenyl-Trichloroethane resistance in field populations PMID: 20585622
  9. Caffeine, DDT and phenobarbital fail to induce upstream DNAs of Cyp6g1. PMID: 20002224
  10. results show that DDT-R, a gene conferring resistance to DDT, is associated with overtranscription of a single cytochrome P450 gene, Cyp6g1 PMID: 12351787
  11. Data show that Cyp6g1 itself may not have to be overexpressed for DDT resistance to occur. PMID: 17134855
  12. Cis-regulatory elements in the accord retrotransposon result in tissue-specific expression of the Drosophila melanogaster insecticide resistance gene Cyp6g1. PMID: 17179088
  13. when levels of Cyp6g1 gene were manipulated in just the Malpighian tubules of adult Drosophila, the survival of the whole insect after 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) challenge was altered. PMID: 17488889
  14. CYP6G1 is capable of metabolising at least two insecticides. PMID: 17912692

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Subcellular Location
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Protein Families
Cytochrome P450 family
Database Links

KEGG: dme:Dmel_CG8453

STRING: 7227.FBpp0087100

UniGene: Dm.3866

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