cusF Antibody

1. We therefore recommend the use of CusF as a viable alternative to MBP or GST as a fusion protein/affinity tag for the production of soluble recombinant proteins in E. coli. PMID: 26805756
2. Arabidopsis with cell wall-, cytosol- and vacuole-targeted CusF had increased Cu accumulation; in particular, plants expressing CusF in the cytoplasm transport Cu from roots to shoots more efficiently than plants with cell wallor vacuole-targeted CusF. PMID: 24951313
3. N-terminal region of CusB is sufficient for metal binding and metal transfer with the metallochaperone CusF. PMID: 22812620
4. Data reveal that N-terminal region of CusB interacts with metal-binding face of CusF; these proteins transiently interact in a metal-dependent fashion. PMID: 21323389
5. Electron paramagnetic resonance (EPR) spectroscopy has been used to structurally characterize the copper-binding site in CusF protein from Escherichia coli PMID: 15770503
6. CusF has a topology and metal binding site that are unique among copper proteins and provide insight into its function. PMID: 16060662
7. The binding of CuI and AgI to CusF using isotheromal titration calorimetry and NMR spectroscopy is reported. PMID: 16964970
8. In the CusF-Ag(I) structure, Ag(I) is coordinated by two methionines and a histidine, with a nearby tryptophan capping the metal site. Measurements on the CusF-Cu(I) complex show a similar environment for Cu(I). PMID: 17893365
9. CusF uses a new metal recognition site wherein Cu(I) is tetragonally displaced from a Met2His ligand plane toward a conserved tryptophan PMID: 18157124

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KEGG: ecj:JW0562

STRING: 316385.ECDH10B_0639