dally Antibody

Code CSB-PA630501XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) dally Polyclonal antibody
Uniprot No.
Target Names
dally
Alternative Names
CG4974 antibody; dally antibody; Dally protein antibody; DALY_DROME antibody; Division abnormally delayed protein antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) dally protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Cell surface proteoglycan that bears heparan sulfate. Required for cell division patterning during post-embryonic development of the nervous system.
Gene References into Functions
  1. dally is essential for normal follicle stem cell maintenance. PMID: 29632032
  2. Autonomous and nonautonomous effects on Drosophila tissue growth rely on the up-regulation of the proteoglycan Dally. Dally also contributes to integrating nutrient sensing and organ scaling, the fitting of pattern to size. PMID: 26313758
  3. findings demonstrate that the glypican dally regulates formation of the follicular gradient of the Upd morphogen, Upd PMID: 23093424
  4. Dally is necessary to sequester Hedgehog at the cell surface and to promote Hedgehog internalisation with its receptor. PMID: 22872085
  5. the long-range activity of Hedgehog is regulated in the apical extracellular space by the glypican Dally and the hydrolase Notum PMID: 20412775
  6. The gene encodes an integral membrane proteoglycan of the glypican family that affects a number of patterning events during both embryonic and larval development. PMID: 11801730
  7. role in Hedgehog signal transduction in Drosophila embryo. PMID: 14602684
  8. Dally, could act as a classical co-receptor. PMID: 15647318
  9. Expression of a secreted form of Dally, a Drosophila glypican, induces overgrowth phenotype by affecting action range of Hedgehog. PMID: 15963974
  10. Heparan sulfate modification of Dally is not required for all in vivo activities; significant functional capacity resides in the protein core. PMID: 17055473
  11. Dally is required for short-range BMP signaling in germline but not in somatic cells. PMID: 19793889
  12. Dally is required for restriction of the female germline stem cell niche space. PMID: 19948496

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Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.
Protein Families
Glypican family
Database Links

KEGG: dme:Dmel_CG4974

STRING: 7227.FBpp0297069

UniGene: Dm.2277

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