Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DOT1 Polyclonal antibody

Uniprot No.

Q04089

Target Names

DOT1

Alternative Names

DOT1 antibody; KMT4 antibody; PCH1 antibody; YDR440W antibody; D9461.26Histone-lysine N-methyltransferase antibody; H3 lysine-79 specific antibody; EC 2.1.1.360 antibody; Disrupter of telomere silencing protein 1 antibody; Histone H3-K79 methyltransferase antibody; H3-K79-HMTase antibody; Lysine N-methyltransferase 4 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DOT1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones. Can bind to DNA (in vitro).

Gene References into Functions

and Dot1 cooperate to drive senescence PMID: 29649255
Findings suggest that Dot1p-mediated histone chaperone activity controls nucleosome dynamics in transcribed regions. PMID: 29339748
Dot1 histone methyltransferases share a distributive mechanism but have highly diverged catalytic properties PMID: 25965993
Dot1 has a role in histone H3K79 methylation that promotes the formation of meiotic double-strand breaks in the absence of histone H3K4 methylation PMID: 24797370
Dot1-dependent H3K79me also promotes Hop1 activation and its proper distribution along zip1 meiotic chromosomes, at least in part, by regulating Pch2 localization PMID: 23382701
histone methyltransferase Dot1 mediates global genomic repair by methylating histone H3 on lysine 79. PMID: 21460225
loss of Dot1 did not globally alter Sir2 or Sir3 occupancy in subtelomeric regions, but only led to some telomere-specific changes PMID: 21474073
contributes to the regulation of DNA damage tolerance and modulates the response to the alkylating agent MMS through its catalytic activity on H3K79. PMID: 20674515
Taken together, these results indicate that a direct interaction between the lysine-rich region of Dot1p and the ubiquitin of H2Bub is required for H2Bub-mediated trans-tail regulation. PMID: 20678485
the relative abundance of the two Dot1 isoforms changed under nutrient-limiting conditions PMID: 19778927
Dot1-dependent histone H3 methylation has a role in G1 and S phase DNA damage checkpoint functions of Rad9 PMID: 16166626
a dot1 null mutation and a histone H3 point mutation at lysine-79 cause increased sensitivity to UV radiation, suggesting that lysine-79 methylation is important for efficient repair of UV damage PMID: 17267293
Loss of DOT1 influences a Rad50-dependent nuclease, leading to more rapid accumulation of ssDNA. PMID: 18418382
Dot1 inhibits translesion synthesis (TLS) by Polzeta/Rev1 and that the methyl methanesulfonate resistance observed in the dot1 mutant results from the enhanced TLS activity. PMID: 18562671
Histone H2B monoubiquitination by Rad6/Bre1 is required for the trimethylation of both histone H3K4 and H3K79 by Dot1 methyltransferase. PMID: 19667127

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Subcellular Location

Nucleus.

Protein Families

Class I-like SAM-binding methyltransferase superfamily, DOT1 family

Database Links

KEGG: sce:YDR440W

STRING: 4932.YDR440W

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DOT1 Antibody

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