Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) dps Polyclonal antibody

Uniprot No.

P0ABT2

Target Names

dps

Alternative Names

dps antibody; pexB antibody; vtm antibody; b0812 antibody; JW0797DNA protection during starvation protein antibody; EC 1.16.-.- antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) dps protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

During stationary phase, binds the chromosome non-specifically, forming a highly ordered and stable dps-DNA co-crystal within which chromosomal DNA is condensed and protected from diverse damages. It protects DNA from oxidative damage by sequestering intracellular Fe(2+) ion and storing it in the form of Fe(3+) oxyhydroxide mineral, which can be released after reduction. One hydrogen peroxide oxidizes two Fe(2+) ions, which prevents hydroxyl radical production by the Fenton reaction. Dps also protects the cell from UV and gamma irradiation, iron and copper toxicity, thermal stress and acid and base shocks. Also shows a weak catalase activity.

Gene References into Functions

A double-alanine mutant of E. coli Dps protein is unable to assemble into a cage due to disruption of key protein-protein interactions. Randomization of residues surrounding the double mutation afforded a repacked interface and proteins with recovered cage formation. PMID: 29276894
three new candidates of E. coli nucleoid, namely DNA-binding protein from starved cells (Dps), host factor for phage Qbeta (Hfq) and suppressor of td(-) phenotype A (StpA) PMID: 26208826
Oxidative stress response proteins such as AhpF, Dps, and Fur, all of which belong to the oxyR regulon, were upregulated in Escherichia coli isolates from mice fed the lactose-rich diet. PMID: 22427493
study investigated the role key protein-protein interactions play in the assembly and stability of the DPS mini-ferritin PMID: 21898653
Unexpectedly, the authors found that dps expression is directly repressed by Mn(2+)-MntR. PMID: 21239586
In contrast, cells lacking Dps exhibited increased cellular copper concentrations compared to their wild-type parent. PMID: 19231146
We describe Escherichia coli survival following neutrophil phagocytosis among three mutants deleted respectively for araB, dps or oxyR. PMID: 15507758
Data suggest thatDps acts as a checkpoint during oxidative stress to reduce initiations, providing an opportunity for mechanisms to repair oxidative DNA damage. PMID: 18284581
Fis and H-NS selectively repress the dps promoter, preventing transcription initiation by RNA polymerase. PMID: 18452510
Dps protected DNA from acid damage by binding to DNA. Dps maintains or forms alpha-helices that are important for Dps-DNA complex formation. PMID: 18922164

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Subcellular Location

Cytoplasm, nucleoid.

Protein Families

Dps family

Database Links

KEGG: ecj:JW0797

STRING: 316385.ECDH10B_0880

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dps Antibody

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