ettA Antibody

Code CSB-PA364392XA01ENV
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Product Details

Full Product Name Rabbit anti-Escherichia coli (strain K12) ettA Polyclonal antibody
Uniprot No. P0A9W3
Target Names ettA
Alternative Names ettA antibody; yjjK antibody; b4391 antibody; JW4354Energy-dependent translational throttle protein EttA antibody; EC 3.6.1.3 antibody; Translational regulatory factor EttA antibody
Raised in Rabbit
Species Reactivity Escherichia coli (strain K12)
Immunogen Recombinant Escherichia coli (strain K12) ettA protein
Immunogen Species Escherichia coli (strain K12)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (14-16 weeks)

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Target Background

Function
(From Uniprot)
A translation factor that gates the progression of the 70S ribosomal initiation complex (IC, containing tRNA(fMet) in the P-site) into the translation elongation cycle by using a mechanism sensitive to the ATP/ADP ratio. Binds to the 70S ribosome E-site where it modulates the state of the translating ribosome during subunit translocation. Stimulates dipeptide bond synthesis in the presence of ATP (cell in high energy state), but inhibits dipeptide synthesis in the presence of ADP (cell in low energy state), and thus may control translation in response to changing ATP levels (including during stationary phase). Following ATP hydrolysis is probably released allowing the ribosome to enter the elongation phase. ATPase activity is stimulated in the presence of ribosomes. Its specificity for the IC may be conferred by its recognition of features unique to tRNA(fMet).
Gene References into Functions
  1. The ATP-bound form of EttA binds to the ribosomal tRNA-exit site, where it forms bridging interactions between the ribosomal L1 stalk and the tRNA bound in the peptidyl-tRNA-binding site. PMID: 24389465
  2. EttA (also known as YjjK) is involved in regulating protein synthesis in energy-depleted cells, which have a low ATP/ADP ratio. PMID: 24389466
Subcellular Location Cytoplasm.
Protein Families ABC transporter superfamily, ABCF family, Translational throttle EttA subfamily
Database Links

KEGG: ecj:JW4354

STRING: 316385.ECDH10B_4549

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