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| Code | CSB-PA364392XA01ENV |
| Size | Enquire |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Full Product Name | Rabbit anti-Escherichia coli (strain K12) ettA Polyclonal antibody |
| Uniprot No. | P0A9W3 |
| Target Names | ettA |
| Alternative Names | ettA antibody; yjjK antibody; b4391 antibody; JW4354Energy-dependent translational throttle protein EttA antibody; EC 3.6.1.3 antibody; Translational regulatory factor EttA antibody |
| Raised in | Rabbit |
| Species Reactivity | Escherichia coli (strain K12) |
| Immunogen | Recombinant Escherichia coli (strain K12) ettA protein |
| Immunogen Species | Escherichia coli (strain K12) |
| Conjugate | Non-conjugated |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification Method | Protein A/G |
| Concentration | It differs from different batches. Please contact us to confirm it. |
| Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Form | Liquid |
| Tested Applications | ELISA, WB (ensure identification of antigen) |
| Protocols | ELISA Protocol |
| Troubleshooting and FAQs | Antibody FAQs |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Value-added Deliverables | ① 200ug * antigen (positive control); ② 1ml * Pre-immune serum (negative control); |
| Quality Guarantee | ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection; ② ELISA titer can be guaranteed 1: 64,000; ③ WB validation with antigen can be guaranteed positive; |
| Lead Time | Made-to-order (14-16 weeks) |
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| Function (From Uniprot) |
A translation factor that gates the progression of the 70S ribosomal initiation complex (IC, containing tRNA(fMet) in the P-site) into the translation elongation cycle by using a mechanism sensitive to the ATP/ADP ratio. Binds to the 70S ribosome E-site where it modulates the state of the translating ribosome during subunit translocation. Stimulates dipeptide bond synthesis in the presence of ATP (cell in high energy state), but inhibits dipeptide synthesis in the presence of ADP (cell in low energy state), and thus may control translation in response to changing ATP levels (including during stationary phase). Following ATP hydrolysis is probably released allowing the ribosome to enter the elongation phase. ATPase activity is stimulated in the presence of ribosomes. Its specificity for the IC may be conferred by its recognition of features unique to tRNA(fMet). |
| Gene References into Functions |
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| Subcellular Location | Cytoplasm. |
| Protein Families | ABC transporter superfamily, ABCF family, Translational throttle EttA subfamily |
| Database Links |
KEGG: ecj:JW4354 STRING: 316385.ECDH10B_4549 |
