Recombinant Escherichia coli Uncharacterized ABC transporter ATP-binding protein YjjK(yjjK)

Code CSB-YP364392ENV
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Source Yeast
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Code CSB-EP364392ENV
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Source E.coli
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Code CSB-EP364392ENV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP364392ENV
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Source Baculovirus
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Code CSB-MP364392ENV
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ettA
Uniprot No. P0A9W3
Alternative Names ettA; yjjK; b4391; JW4354Energy-dependent translational throttle protein EttA; EC 3.6.1.3; Translational regulatory factor EttA
Species Escherichia coli (strain K12)
Expression Region 2-555
Target Protein Sequence AQFVYTMHR VGKVVPPKRH ILKNISLSFF PGAKIGVLGL NGAGKSTLLR IMAGIDKDIE GEARPQPDIK IGYLPQEPQL NPEHTVRESI EEAVSEVVNA LKRLDEVYAL YADPDADFDK LAAEQGRLEE IIQAHDGHNL NVQLERAADA LRLPDWDAKI ANLSGGERRR VALCRLLLEK PDMLLLDEPT NHLDAESVAW LERFLHDFEG TVVAITHDRY FLDNVAGWIL ELDRGEGIPW EGNYSSWLEQ KDQRLAQEAS QEAARRKSIE KELEWVRQGT KGRQSKGKAR LARFEELNST EYQKRNETNE LFIPPGPRLG DKVLEVSNLR KSYGDRLLID DLSFSIPKGA IVGIIGPNGA GKSTLFRMIS GQEQPDSGTI TLGETVKLAS VDQFRDSMDN SKTVWEEVSG GLDIMKIGNT EMPSRAYVGR FNFKGVDQGK RVGELSGGER GRLHLAKLLQ VGGNMLLLDE PTNDLDIETL RALENALLEF PGCAMVISHD RWFLDRIATH ILDYQDEGKV EFFEGNFTEY EEYKKRTLGA DALEPKRIKY KRIAK
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
A translation factor that gates the progression of the 70S ribosomal initiation complex (IC, containing tRNA(fMet) in the P-site) into the translation elongation cycle by using a mechanism sensitive to the ATP/ADP ratio. Binds to the 70S ribosome E-site where it modulates the state of the translating ribosome during subunit translocation. Stimulates dipeptide bond synthesis in the presence of ATP (cell in high energy state), but inhibits dipeptide synthesis in the presence of ADP (cell in low energy state), and thus may control translation in response to changing ATP levels (including during stationary phase). Following ATP hydrolysis is probably released allowing the ribosome to enter the elongation phase. ATPase activity is stimulated in the presence of ribosomes. Its specificity for the IC may be conferred by its recognition of features unique to tRNA(fMet).
Gene References into Functions
  1. The ATP-bound form of EttA binds to the ribosomal tRNA-exit site, where it forms bridging interactions between the ribosomal L1 stalk and the tRNA bound in the peptidyl-tRNA-binding site. PMID: 24389465
  2. EttA (also known as YjjK) is involved in regulating protein synthesis in energy-depleted cells, which have a low ATP/ADP ratio. PMID: 24389466
Subcellular Location Cytoplasm.
Protein Families ABC transporter superfamily, ABCF family, Translational throttle EttA subfamily
Database Links

KEGG: ecj:JW4354

STRING: 316385.ECDH10B_4549

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