Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RAD27 Polyclonal antibody

Uniprot No.

P26793

Target Names

RAD27

Alternative Names

RAD27 antibody; FEN1 antibody; RTH1 antibody; YKL113C antibody; YKL510Flap endonuclease 1 antibody; FEN-1 antibody; EC 3.1.-.- antibody; Flap structure-specific endonuclease 1 antibody; RAD2 homolog nuclease 1 antibody; RTH1 nuclease antibody; Structure-specific endonuclease RAD27 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RAD27 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Gene References into Functions

Data show that wild-type Rad27 proteins are more stably associated with chromatin than tailless Rad27. PMID: 27759916
our findings underscore the importance of Rad27p in the maintenance of mtDNA, and demonstrate that it participates in multiple DNA repair pathways in mitochondria, unlinked to nuclear phenotypes. PMID: 28450457
C-terminal 16-amino acid fragment of Rad27 was sufficient and necessary for the stimulation of both Rad27 and Dna2. PMID: 22235122
Rad27p/FEN1 is localized in the mitochondrial compartment of both yeast and mice and that Rad27p has a significant role in maintaining mtDNA integrity. PMID: 19699691
Pathological interactions between the rad27Delta and specific nupDelta mutations result from the accumulation of unrepaired DNA damages. PMID: 15725626
the activity of Rad27p was critical for achieving full chronological lifespan PMID: 17506834
Biochemical fractionation studies identified FEN1 (Rad27) as another required DNA large loop repair component. PMID: 18628298
Dna2 enables FEN1 to cleave RPA-coated flaps. In summary, RPA, Dna2, and FEN1 have evolved highly coordinated binding properties enabling one protein to succeed the next for proper and efficient Okazaki flap processing. PMID: 19179330

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Subcellular Location

Nucleus, nucleolus. Nucleus, nucleoplasm. Mitochondrion. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.

Protein Families

XPG/RAD2 endonuclease family, FEN1 subfamily

Database Links

KEGG: sce:YKL113C

STRING: 4932.YKL113C

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RAD27 Antibody

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