FLO11 Antibody

Code CSB-PA357442XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FLO11 Polyclonal antibody
Uniprot No.
Target Names
FLO11
Alternative Names
FLO11 antibody; MAL5 antibody; MUC1 antibody; S1 antibody; S2 antibody; YIR019CFlocculation protein FLO11 antibody; Flo11p antibody; Flocculin-11 antibody; Mucin-like protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FLO11 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Homophilic binding protein that enables kin discrimination in heterogeneous yeast populations by mediating homotypic cell-cell interactions during flocculation, a reversible and asexual process in which cells adhere to form aggregates (flocs). Plays a role in cell-substrate adhesion, haploid invasive growth, diploid pseudohyphae formation and biofilm (flor) development. Adhesive activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose.
Gene References into Functions
  1. by deleting many of the known transcriptional activators of invasion, as well as the gene encoding the cell surface protein Flo11 from five relevant segregants and showing that these individuals differ in the genes they require for invasion PMID: 26232408
  2. Interactions by the fungal Flo11 adhesin depend on a fibronectin type III-like adhesin domain girdled by aromatic bands. PMID: 25960408
  3. Flo11 downregulation causes filamentous and invasive growth of rho mutants. PMID: 23172851
  4. findings show that Flo11p is covalently associated with the cell wall and shed into the extracellular matrix of the growing mat. PMID: 21908597
  5. Surface plasmon resonance analysis showed that the N-terminal domain of Flo11p self-interacts and explains the cell-cell interaction capacity of FLO11-expressing cells. PMID: 22129043
  6. Adhesin Flo11p participates in the formation of extracellular fibers which in biofilms interconnect oval cells in the upper layer and pseudohyphae in the roots. PMID: 21875945
  7. A flo11Delta mutant, which fails to form pseudohyphae, yielded normal sporulation in cultures, but was defective for minicolony sporulation. PMID: 21205160
  8. FLO11 is an essential gene involved in pellicle formation by wine spoilage wild yeasts. PMID: 20926338
  9. Two alleles of 3.1 and 5.0 kb were cloned into strain BY4742 to compare the FLO11-associated phenotypes in the same genetic background. There is a significant correlation between biofilm-forming ability and FLO11 length. PMID: 19729408
  10. Results describe the ability of tailor-made zinc finger proteins to activate the expression of the FLO11 gene in Saccharomyces cerevisiae , with phenotypic consequences. PMID: 17710146
  11. FLO11 has a role in biofilm formation in Saccharomyces cerevisiae. PMID: 15321692
  12. required for air-liquid interfacial biofilm formation during growth of wine strain on ethanol PMID: 15932987
  13. Flo11p-dependent flocculation revealed is dependent on calcium, occurs only at cell densities greater than 1 x 10(8) ml(-1), and only occurs at acidic pH. PMID: 16043420
  14. disruptant (Deltaflo11) shows lower hydrophobicity of cell surface than the wild type PMID: 16556982
  15. analysis of adaptive evolution mutations in the FLO11 gene PMID: 16844788
  16. we present a modular steady state approach to quantify the network comprising of cAMP-PKA and MAP kinase pathways involved in the regulation of FLO11, a gene which is required for pseudohyphae growth in Saccharomyces cerevisiae under nitrogen starvation. PMID: 16863676
  17. These results indicate that Opi1p affects mat formation and invasive growth by participating in the regulation of FLO11. PMID: 16896211
  18. Deletion of CPC2/ASC1 abolished amino acid starvation-induced adhesive growth and impaired basal expression of FLO11 and its activation upon starvation in haploid cells. PMID: 17704055
  19. Flo11/Muc1 is a Saccharomyces cerevisiae mannoprotein with homotypic properties of adhesion PMID: 17921350
  20. biofilm formation and sliding motility were shown to be strictly dependent on the cell-surface adhesin Flo11p PMID: 17947402
  21. It is therefore concluded that low levels of FLO11 transcripts are essential and sufficient for derepression of FLO11 expression and adhesive growth during amino acid starvation. PMID: 17999676
  22. Several genes whose function was essential to maintain the expression of FLO11, were isolated. PMID: 18202364
  23. global signaling pathways respond with specific sensitivity to regulate the expression of FLO11 under nitrogen limitation. PMID: 18301741
  24. study reports that, under optimal laboratory conditions, the repeats domain of the FLO11 gene in wild yeasts is unstable; length variations in this gene provide a combinatorial diversity which may contribute to rapid adaptation to fluctuating environments PMID: 19160455
  25. FLO11 switch between a silenced and competent promoter state in a random and independent fashion. PMID: 19798446
  26. demonstrate that Rpd3L activates FLO11 transcription via its repressive effects on one of the cis-acting noncoding RNAs that itself negatively regulates FLO11 transcription. PMID: 19805129

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Subcellular Location
Secreted, cell wall. Membrane; Lipid-anchor, GPI-anchor.
Protein Families
Flocculin family
Database Links

KEGG: sce:YIR019C

STRING: 4932.YIR019C

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