Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GET3 Polyclonal antibody

Uniprot No.

Q12154

Target Names

GET3

Alternative Names

GET3 antibody; ARR4 antibody; YDL100C antibody; D2371ATPase GET3 antibody; EC 3.6.-.- antibody; Arsenical pump-driving ATPase antibody; Arsenite-stimulated ATPase antibody; Golgi to ER traffic protein 3 antibody; Guided entry of tail-anchored proteins 3 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GET3 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.

Gene References into Functions

Get3 functions as a redox-regulated chaperone, effectively protecting eukaryotic cells against oxidative protein damage. PMID: 25242142
The crystal structure of the Get3-Get4-Get5 complex in an ATP-bound state is presented. PMID: 24727835
Get3 moves reversibly to deposition sites for protein aggregates, hence supporting the sequestration of tail-anchored proteins under conditions that prevent tail-anchored protein insertion. PMID: 23203805
ATP specifically induces multiple conformational changes in Get3 that culminate in its ATPase activation through tetramerization PMID: 23610396
Get1 uses two interfaces to stabilize the open dimer conformation of Get3. PMID: 22684149
Interaction surface and topology of Get3-Get4-Get5 protein complex, involved in targeting tail-anchored proteins to endoplasmic reticulum PMID: 22190685
crystal structures of Get3 in complex with cytosolic domain of Get1 and Get2 in different states; data show Get1 and Get2 can bind simultaneously to Get3;docking to Get1/2 complex allows for conformational change in Get3 required for TA protein insertion PMID: 21719644
Data provide further evidence for a model in which Get4/5 operates upstream of Get3 and mediates the specific delivery of a TA substrate. PMID: 20554915
the conformational changes to switch the Get3 between the open and closed conformations may facilitate the membrane insertions for TA proteins PMID: 19956640
the Get4-Get5 complex interacts with Sgt2, Get3, and Ydj1 PMID: 20106980
Arr4p antagonizes the function of Gef1p PMID: 16260785
Study shows that Get3, the yeast homolog of the Tail-anchored(TA)-interacting factor Asna1/Trc40, specifically recognizes transmembrane domains of TA proteins destined for the secretory pathway. PMID: 18724936
crystals od Get3 diffracted to 2.7 A resolution using a synchrotron X-ray source PMID: 19407384
crystal structures of yeast Get3 in 'open' (nucleotide-free) and 'closed' (ADP.AlF(4)(-)-bound) dimer states PMID: 19675567
determined three crystal structures in apo and ADP forms from Saccharomyces cerevisae (ScGet3-apo) and Aspergillus fumigatus (AfGet3-apo and AfGet3-ADP). PMID: 19706470
Structural and functional analysis of yeast Get3 provides a molecular model for nucleotide-regulated binding and release of tail-anchored proteins during targeting to the ER membrane. PMID: 19675567

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Subcellular Location

Cytoplasm. Endoplasmic reticulum. Golgi apparatus. Note=GET1 and GET2 are required for targeting GET3 to the endoplasmic reticulum.

Protein Families

ArsA ATPase family

Database Links

KEGG: sce:YDL100C

STRING: 4932.YDL100C

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GET3 Antibody

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