Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GET4 Polyclonal antibody

Uniprot No.

Q7L5D6

Target Names

GET4

Alternative Names

C7orf20 antibody; CGI 20 antibody; CGI20 antibody; Conserved edge expressed protein antibody; Conserved edge protein antibody; Conserved edge-expressed protein antibody; GET 4 antibody; GET4 antibody; GET4_HUMAN antibody; Golgi to ER traffic protein 4 homolog (S. cerevisiae) antibody; Golgi to ER traffic protein 4 homolog antibody; Transmembrane domain recognition complex 35 kDa subunit antibody; Transmembrane domain recognition complex 35kDa antibody; TRC35 antibody; UPF0363 protein C7orf20 antibody

Raised in

Rabbit

Species Reactivity

Homo sapiens (Human)

Immunogen

Recombinant Homo sapiens (Human) GET4 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates in their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation. The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum. Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome. Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum. The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome.

Gene References into Functions

Both TRC35 and Ubl4A have distinct C-terminal binding sites on Bag6 defining a minimal Bag6 complex. PMID: 25535373
The crystal structure of the Get3-Get4-Get5 complex in an ATP-bound state is presented. PMID: 24727835
Data indicate that BCL2-associated athanogene 6 (BAG6) appears to be the central component for the process, as depletion of BAG6 leads to the loss of both UBL4A and GET4 proteins and resistance to cell killing by DNA-damaging agents. PMID: 23723067
Data indicate that the Bag6-Ubl4A-Trc35 complex is localized to the endoplasmic reticulum (ER) membrane to regulate ER-associated degradation (ERAD). PMID: 23665563
SGTA recognizes a noncanonical ubiquitin-like domain in the Bag6-Ubl4A-Trc35 complex to promote endoplasmic reticulum-associated degradation. PMID: 23246001

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Subcellular Location

Cytoplasm, cytosol.

Protein Families

GET4 family

Database Links

HGNC: 21690

OMIM: 612056

KEGG: hsa:51608

STRING: 9606.ENSP00000265857

UniGene: Hs.107387

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GET4 Antibody

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