GPA1 Antibody

Code CSB-PA323565XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GPA1 Polyclonal antibody
Uniprot No.
Target Names
GPA1
Alternative Names
GPA1 antibody; At2g26300 antibody; T1D16.6 antibody; Guanine nucleotide-binding protein alpha-1 subunit antibody; GP-alpha-1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GPA1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Exhibits a fast rate of basal nucleotide exchange. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Together with GCR1, may regulate the cell cycle via a signaling cascade that uses phosphatidylinositol-specific phospholipase C (PI-PLC) as an effector and inositol 1,4,5-trisphosphate (IP(3)) as a second messenger. Promotes abscisic acid (ABA) responses in guard cells. Involved in the blue light (BL) signaling. Together with GCR1 and ADT3, required for BL-mediated synthesis of phenylpyruvate and subsequently of phenylalanine (Phe), in etiolated seedlings. Modulates root architecture (e.g. lateral root formation). Negatively regulated by RGS1. In collaboration with CAND2/PMTR1, regulates the melatonin-mediated stomatal closure involving H(2)O(2) and Ca(2+) signals.
Gene References into Functions
  1. Found 394 GPA1-regulated genes spanning 79 processes, including biotic and abiotic stresses, development, flavonoid biosynthesis, transcription factors, transporters and nitrate/phosphate responses. Many are unknown in published gpa1 mutant analyses. PMID: 26346778
  2. Galpha mediates ethylene-induced stomatal closure via H2O2 production, and acts downstream of RAN1, ETR1, ERS1, EIN4 and CTR1 and upstream of EIN2, EIN3 and ARR2. The data also show that ETR1 and ERS1 mediate both ethylene and H2O2 signalling in guard cells. PMID: 25704455
  3. AGG3, GPA1 and AGB1 act in the same genetic pathway to influence organ size and shape in A. thaliana. PMID: 22380792
  4. GCR1 and GPA1 are involved in N-acyl-homoserine lactones -mediated elongation of Arabidopsis roots. PMID: 22250582
  5. These findings establish a signaling pathway leading to ultraviolet-B-induced stomatal closure that involves GPA1-dependent activation of hydrogen peroxide production and subsequent Nia1-dependent nitric oxide accumulation. PMID: 23341360
  6. Both cry1 and gpa1 showed reduced accumulation of anthocyanin under blue light. Both gpa1 and cry1 mutants showed reduced GTP-binding activity. It is propsed that cry1-mediated post-translational modification of GPA1 alters its GTP-binding activity. PMID: 22855128
  7. Absence of GPA1 interrupts abscisic acid signalling between ABA reception and reactive oxygen species production, with a consequent impairment in Ca(2+)-channel activation. PMID: 21262908
  8. Data show that the helical domain of AtGPA1 was necessary for self-activation. PMID: 21304159
  9. GPA1, AGB1, AGG and RGS1 are involved in glucose attenuation of auxin-mediated lateral root formation. PMID: 20862254
  10. A new mechanism of G-protein signaling is revealed, in which Gbeta regulates gene expression identically in the presence or absence of Galpha. PMID: 20531402
  11. GCR1 may act as a negative regulator of GPA1-mediated abscisic acid responses in guard cells PMID: 15155892
  12. The effect of extracellular calmodulin on stomatal closure in GPA1 mutant Arabidopsis. PMID: 15557100
  13. G-protein-coupled receptor 1, G-protein Galpha-subunit 1, and prephenate dehydratase 1 form all of or part of a signal transduction mechanism responsible for the light-mediated synthesis of phenylpyruvate and phenylalanine. PMID: 16415218
  14. the Gbetagamma complex serves a signaling function in the ER independent of its function in the Galphabetagamma heterotrimer PMID: 17360436
  15. This research provides evidence of the functional association of alpha and beta with the gamma subunits in Arabidopsis, while also suggesting that plasma membrane localization may be critical for function of plant heterotrimeric G proteins. PMID: 17383830
  16. may participate in pollen germination through modulation of the hyperpolarization-activated Ca(2+) channel in the plasma membrane of pollen cells PMID: 17953540
  17. These results provided the first genetic evidence that G-protein- and brassinosteroid-mediated pathways may be converged to modulate cell proliferation in a cell/tissue-specific manner. PMID: 18499742
  18. the stomatal density in Arabidopsis is modulated by GPA1 and AGB1 in an antagonistic manner. PMID: 18834874
  19. GPA1 is a positive regulator for chloroplast development in thf1 mutants. PMID: 19228339
  20. Extracellular calmodulin-mediated NO generation is regulated by GPA1, the Galpha-subunit of heterotrimeric G protein. PMID: 19321706
  21. Jasmonate-regulated gene expression is mediated by gpa1. PMID: 19342430

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Subcellular Location
Cell membrane.
Protein Families
G-alpha family
Tissue Specificity
More abundant in roots and/or leaves.
Database Links

KEGG: ath:AT2G26300

STRING: 3702.AT2G26300.1

UniGene: At.25148

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