Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HTB1 Polyclonal antibody

Uniprot No.

P02293

Target Names

HTB1

Alternative Names

H2B1_YEAST antibody; Histone H2B.1 antibody; Histone H2B.2 antibody; HTB 1 antibody; HTB 2 antibody; HTB1 antibody; Htb1p antibody; HTB2 antibody; Htb2p antibody; SPT 12 antibody; SPT12 antibody; Suppressor of Ty protein 12 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HTB1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene References into Functions

This study not only demonstrates the applications of the versatile histone library, but also reveals many previously unknown functions of histone H2A and H2B. PMID: 29038170
Data indicate that histone H2B ubiquitylation (H2Bub) and Asf1 cooperatively promote transcriptional silencing at telomeres and mating loci. PMID: 28520954
A Nap1 dimer provides an acidic binding surface and asymmetrically engages a single H2A-H2B heterodimer. PMID: 27225933
Chd1 takes part in chromatin reorganization surrounding transcribing RNA polymerase II (Pol II) in asymmetric nucleosomes; ubiquitin-conjugated histone H2B stimulates nucleosome sliding by Chd1. PMID: 28032848
decreased CKII function in vivo results in decreased levels of histone H2B lysine 123 monoubiquitylation, a modification dependent on FACT and PAF-C. PMID: 27143358
Nap1 is essential for maintaining proper chromatin composition and modulating the exchange of H2A-H2B in vivo. PMID: 26884462
A role for the H2B nucleosome acidic patch in recruitment of histone modification machinery and maintenance of transcriptional integrity. PMID: 26241481
FACT, a heterodimer of Spt16 and Pob3, disrupts nucleosome structure by binding H2A-H2B with conserved peptide motifs. PMID: 26455391
A histone modification domain (HMD), when expressed as the only source of Rtf1, can promote H3 K4 and K79 methylation and H2B K123 ubiquitylation in yeast. PMID: 22699496
lysine 123 affects transcription in yeast primarily because of it being a site of ubiquitylation in HTB1 PMID: 17447102
A continued accumulation of the histone mRNA HTB1 appears to result from a delay in exit from S-phase in rrp6 deleted cells. PMID: 17855393
analysis of the NMR structure of budding yeast chaperone Chz1 complexed with histones H2A.Z-H2B PMID: 18641662
A yeast strain unable to be ubiquitylated on H2B (K123R) is still proficient for H3 trimethylation at both K4 and K79, indicating that H3 methylation status is not solely dependent on H2B ubiquitylation. PMID: 19255247

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Subcellular Location

Nucleus. Chromosome.

Protein Families

Histone H2B family

Database Links

KEGG: sce:YDR224C

STRING: 4932.YDR224C

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HTB1 Antibody

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