HSFA2 Antibody

Code CSB-PA528052XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HSFA2 Polyclonal antibody
Uniprot No.
Target Names
HSFA2
Alternative Names
HSFA2 antibody; HSF04 antibody; At2g26150 antibody; T19L18.4 antibody; Heat stress transcription factor A-2 antibody; AtHsfA2 antibody; AtHsf-04 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana HSFA2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Transcriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE). Involved in heat stress responses. Seems to be involved in other environmental stress responses. Activates ascorbate peroxidase 2 (APX2) in addition to several heat shock protein (HSPs).
Gene References into Functions
  1. Study demonstrated the flexible mode of heat response pathways involving bZIP28, HSFA2 and ROS-dependent signals. PMID: 28873003
  2. LIN treatment rapidly increases the levels of aggregated proteins in the chloroplast, unleashing a specific retrograde signaling pathway that up-regulates expression of ClpB3 and other nuclear genes encoding plastidial chaperones.Expression of HsfA2 and cpUPR-related target genes is independent of GUN1, a central integrator of retrograde signaling pathways PMID: 28937985
  3. transcriptional responses of the OPDA-responsive genes HsfA2 and DREB2A to the protein synthesis inhibitor cycloheximide and to the HSP90 inhibitor geldanamycin PMID: 25036962
  4. Self-regulation of HsfA2 expression is through a small truncated HsfA2 isoform encoded by the splice variant. PMID: 23503691
  5. Data indicate that cngc16 mutant pollen have attenuated expression of several heat-stress response genes, including two heat shock transcription factor genes, HsfA2 and HsfB1. PMID: 23370720
  6. We have established a previously unrecognized molecular connection between the downregulation of RPS1 expression in chloroplast and the activation of HsfA2-dependent heat-responsive genes in nucleus, which is required for heat tolerance in higher plants. PMID: 22570631
  7. These findings indicated that HsfA1d and HsfA1e not only regulate HsfA2 expression but also function as key regulators of the Hsf signaling network in response to environmental stress. PMID: 21471117
  8. The phenotype of seedlings overexpressing AtSUMO1 resembled the phenotype of AtHsfA2 knock out seedlings, which were more sensitive than wild type seedlings to repeated heat treatment. PMID: 20521085
  9. HsfA2 is an important transcription factor that confers anoxia tolerance to Arabidopsis seedlings. PMID: 20089772
  10. The accumulation of polyubiquitinated proteins causes the induction of HsfA2 expression under oxidative stress. PMID: 20147301
  11. AtHsfA2 has role in linking heat shock with oxidative stress signals in Arabidopsis PMID: 16483133
  12. The results of this study indicate that HsfA2 is a key regulator in the induction of the defence system under several types of environmental stress. PMID: 17059409
  13. HsfA2 functions as a heat-inducible transactivator, sustains the expression of Hsp genes, and extends the duration of acquired thermotolerance in Arabidopsis. PMID: 17085506
  14. HsfA2 protects plants against oxidative damage, organelle dysfunction, and subsequent cell death. PMID: 19342427
  15. HsfA2 appears to function not only in the triggering of response to environmental stress, but also in the amplification of the signal in the response. PMID: 19352026

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
HSF family, Class A subfamily
Database Links

KEGG: ath:AT2G26150

STRING: 3702.AT2G26150.1

UniGene: At.38933

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