ISW2 Antibody

1. Histone hyperacetylation does not necessarily block ISWI functions and that ISWI enzymes can function on active chromatin as well. PMID: 26692330
2. the ISWI class of ATP-dependent chromatin remodeling complexes plays a conserved role during aging and in calorie restriction. PMID: 24814484
3. Isw2 showed little evidence of nucleosome disassembly. PMID: 24050724
4. Present evidence that a major targeting mechanism for the Isw2 chromatin remodeling enzyme to specific genomic loci is through sequence-specific transcription factor (TF)-dependent recruitment. PMID: 23478442
5. ISW2 has a conserved domain called SLIDE (SANT-like ISWI domain) that binds to extranucleosomal DNA ~19 base pairs from the edge of nucleosomes. PMID: 23334290
6. The GST-tagged SET-domain polypeptide of human ALL-1 was assayed for binding to assembled mononucleosomes and nucleosome-dimer particles, either intact or remodeled with purified Isw2. PMID: 22583166
7. Linker DNA was found to be critical for the specific docking of ISW2 with nucleosomes as shown by mapping the physical contacts of ISW2 with nucleosomes at base-pair resolution. PMID: 15131696
8. extranucleosomal DNA was required for nucleosome mobilization by ISW2 PMID: 15509805
9. Mutant Isw2p (K215R), but not wild-type Isw2p, is a sensitive chromatin immunoprecipitation (ChIP) reagent for marking sites of Isw2 activity in vivo. PMID: 15833917
10. histone H4 tail, in concert with a favorable length of extranucleosomal DNA, recruits and properly orients ISW2 onto the nucleosome for efficient nucleosome remodeling PMID: 17015471
11. Chromatin-mediated repression by Isw1 and Isw2 may serve to limit activation of CLB2 expression by the Clb2/Cdk1 kinase during G(2)/M and to fully repress expression during G(1). PMID: 17283050
12. Isw2 is required for transcriptional silencing at the rDNA locus. PMID: 17689493
13. The C-terminal domains of Isw2 are involved in anchoring the complex to nucleosomes through their interactions with linker DNA and that they facilitate the movement of DNA along the surface of nucleosomes. PMID: 17908792
14. histone H4 tetra-acetylation was also found to reduce the activity of the Chd1 and Isw2 remodelling enzymes by reducing catalytic turnover PMID: 17949749
15. yIsw2 is capable of translocating along single-stranded DNA in a reaction that is coupled to ATP hydrolysis. PMID: 19203228
16. Data show that the initial binding of ISW2 to extranucleosomal DNA, to the entry site, and near the dyad axis of the nucleosome is enhanced by ATP binding. PMID: 19595716

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KEGG: sce:YOR304W

STRING: 4932.YOR304W