TPK1 Antibody

Code CSB-PA361759XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TPK1 Polyclonal antibody
Uniprot No.
Target Names
TPK1
Alternative Names
TPK1 antibody; PKA1 antibody; SRA3 antibody; YJL164C antibody; J0541 antibody; cAMP-dependent protein kinase type 1 antibody; PKA 1 antibody; EC 2.7.11.11 antibody; CDC25-suppressing protein kinase antibody; PK-25 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TPK1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Gene References into Functions
  1. These results show that rapid activation of the TOR pathway by amino acids is not involved in rapid activation of the PKA pathway and that effects of Gcn2 inactivation as well as leu2 auxotrophy all act independently of the TOR pathway. PMID: 28810702
  2. Long non-coding RNA IRT1 transcription is repressed when nutrients are ample and target of rapamycin complex I (TORC1) and protein kinase A (PKA) are active. PMID: 27520925
  3. protein kinase A (PKA) and target of rapamycin complex I (TORC1) signalling mediate nutrient regulation of IME1 expression. PMID: 27272508
  4. Data indicate that Rgt1 and tpk1/2/3 (cAMP-dependent protein kinases) participate in the control of SUC2 expression. PMID: 25810078
  5. results describe that an autophosphorylation mechanism on Tpk1 controls PKA activity in response to glucose availability PMID: 24947305
  6. Trehalase activation is associated with phosphorylation of typical PKA sites. PMID: 23155055
  7. Tpk1 and Tpk2 interact with Pkh1 through a hydrophobic motif-PDK1-interacting fragment pocket interaction. PMID: 22957732
  8. The authors show that the evolutionarily conserved protein kinases Atg1, target of rapamycin kinase complex I, and protein kinase A (PKA) regulate autophagic flux, whereas autophagy gene induction depends solely on PKA. PMID: 21468027
  9. S. cerevisiae DPMS activity is regulated by the cAMP-dependent protein phosphorylation signal, and the phosphorylation target is serine 141 PMID: 15548536
  10. Tor and cyclic AMP-protein kinase A regulate expression of genes required for cell growth PMID: 15643061
  11. Tpk1p phosphorylation is dynamic and reversible: its up-regulation correlates with a fully fermentative metabolism, while its down-regulation with stationary phase or respiratory metabolism. PMID: 16226873

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Protein kinase superfamily, AGC Ser/Thr protein kinase family, cAMP subfamily
Database Links

KEGG: sce:YJL164C

STRING: 4932.YJL164C

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