Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans unc-43 Polyclonal antibody

Uniprot No.

O62305

Target Names

unc-43

Alternative Names

unc-43 antibody; K11E8.1 antibody; Calcium/calmodulin-dependent protein kinase type II antibody; CaM kinase II antibody; EC 2.7.11.17 antibody; Uncoordinated protein 43 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans unc-43 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Acts in the signaling of a variety of pathways and processes. Phosphorylates 'Ser-319' of daf-16 in response to stress signals, such as heat, starvation and oxidation, which plays a role in prolonging lifespan. Required for viability under chronic osmotic stress in which it acts downstream of osr-1. Has roles in locomotion, oocyte maturation, brood size, egg laying, defecation, meiotic maturation and neuronal cell fate specification. Required for the regulation of synaptic density and neuromuscular junction morphology. Regulates the synaptic trafficking of glr-1. Bidirectional modulator of neurotransmitter release with negative modulatory effects mainly mediated via slo-1 activation. Involved in activation of ADF neurons and increased tph-1 transcription following exposure to pathogenic bacteria which leads to learned olfactory aversion to the bacteria. Implicated in the muscle regulation of spicule protraction. In conjunction with egl-2 has a role in the suppression of mating behavior under food deprivation to encourage foraging. Involved in restricting str-2 expression to only one of the two AWC neurons. May suppress the functional response to an internal pacemaker, perhaps by modulating the activity of the IP3 receptor.

Gene References into Functions

these data implicate the importance of subunit stoichiometry as another regulatory mechanism of PKA activity and lipid metabolism. PMID: 27496951
Results with unc-43 mutants reveal a new function for CaM kinase II in blocking the regulated exocytosis of dense core vesicles before they are transported into axons. PMID: 24653209
UNC-43 functions cell-autonomously in ADF neurons to generate training-dependent enhancement in neuronal activity and tph-1 transcription, and this cell-autonomous function of UNC-43 is required for learning. PMID: 23325232
Studies sugget the effect of CaMKII autophosphorylation at Thr286 on calmodulin trapping that it stabilises the active state and therefore makes the high-affinity binding site accessible. PMID: 22279535
The activity of egg-laying defective protein (EGL)-2 is promoted by calcium/calmodulin-dependent protein kinase II (CaMKII) unc-43. PMID: 21145946
VAB-1 switches from a negative regulator into a redundant positive regulator of oocyte maturation upon binding to MSP. NMR-1 mediates this switch by controlling UNC-43 CaMKII activation at the oocyte cortex PMID: 16267094
comparison of the oligomeric states of the isolated association domains of CaMKII from mouse and Caenorhabditis elegans with that of the holoenzyme PMID: 16441656
presynaptic CaMKII is a bidirectional modulator of neurotransmitter release. PMID: 17898212

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Subcellular Location

Cytoplasm. Cell projection, axon. Perikaryon.

Protein Families

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily

Tissue Specificity

Expressed in the nervous system. Observed in the ADF and AWC neurons. Position in AWC neurons is regulated by microtubules. Localized to clusters in ventral cord neurites which appear to be required for glr-1 trafficking. Also present in oocytes.

Database Links

UniGene: Cel.22903

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unc-43 Antibody

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Target Background