Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LHP1 Polyclonal antibody

Uniprot No.

Q946J8

Target Names

LHP1

Alternative Names

LHP1 antibody; TFL2 antibody; TU8 antibody; At5g17690 antibody; MVA3.4Chromo domain-containing protein LHP1 antibody; Protein LIKE HETEROCHROMATIN PROTEIN 1 antibody; Protein TERMINAL FLOWER 2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana LHP1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Structural component of heterochromatin involved in gene repression, including several floral homeotic genes and FLT that regulates flowering time. Required for maintenance of vernalization-induced repression of FLC. As part of the PRC1-like complex, recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me), leading to epigenetic repression. PcG PRC1 complex maintains the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Transcriptional repressor that binds DNA on GAGA-like motif and 5'-(A/G/T)AACCCTA(A/G)-3' consensus motif in the promoters of target genes. Recognizes and binds histone H3 tails methylated at 'Lys-27' (H3K27me) but depleted in active histone marks such as H3K4me, leading to epigenetic repression. When in complex with LHP1, recognizes and binds histone H3 tails methylated at 'Lys-4' (H3K4me) and 'Lys-27' (H3K27me), mostly corresponding to stress-responsive genes.

Gene References into Functions

a general role of LHP1 from local to higher conformation levels of chromatin configuration to determine its accessibility to define gene expression patterns PMID: 27410265
Neither PRC2 activity nor LHP1 function are a major determinant for H2A monoubiquitination in Arabidopsis thaliana. PMID: 28403905
Arabidopsis (Arabidopsis thaliana) GAGA-motif binding factor protein basic pentacysteine6 (BPC6) interacts with like heterochromatin protein1 (LHP1), a PRC1 component, and associates with vernalization2 (VRN2), a PRC2 component, in vivo. PMID: 26025051
MSI1 serves to link PRC2 to LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), a protein that binds H3K27me3 in vitro and in vivo and is required for a functional polycomb group system. PMID: 23778966
TFL2 is required for normal seedling de-etiolation, greening and shade avoidance response through negative regulation of both PHYB-dependent R light signalling and PHYA-dependent FR light signalling. PMID: 22145973
LIF2, involved in cell identity and cell fate decision, may modulate the activity of LHP1 at specific loci, during specific developmental windows or in response to environmental cues that control cell fate determination.[LIF2] PMID: 21304947
In vivo, TFL2 is targeted to a number of the YUCCA genes in an auxin-dependent fashion revealing a role of TFL2 in auxin regulation, probably as a component of protein complexes affecting transcriptional control. PMID: 21251106
We investigated the influence of heat stress on metabolites in the tu8 (a novel allele of TERMINAL FLOWER2) mutant. [TU8] PMID: 16025407
In transgenic plants expressing the LHP1-GFP fusion protein, two major localization patterns were observed according to cell types suggesting that localization evolves with age or differentiation states. PMID: 16244868
Results imply that LIKE HETEROCHROMATIN PROTEIN1 (LHP1) recruitment to chromatin is mediated through interaction with methyl K9 and that LHP1 controls different nuclear processes via transient binding to its nuclear sites. PMID: 16361394
chromodomain protein LIKE HETEROCHROMATIN PROTEIN (LHP)1 might play a role in vernalization PMID: 16549797
LIKE HETEROCHROMATIN PROTEIN 1 (LHP1) is necessary to maintain the epigenetically repressed state of FLC upon return to warm conditions typical of spring PMID: 16682972
LHP1, like its animal homologues, has a high binding affinity for A/T-rich regions. PMID: 16972870
Recognizes specifically trimethylated Histone H3 in vivo as part of a mechanism that represses the expression of many genes targeted by Polycomb repressive complex 2. PMID: 17542647
Arabidopsis thaliana chromodomain-containing protein LHP1 colocalizes with H3K27me3 genome-wide. The LHP1 chromodomain also binds H3K27me3 with high affinity, suggesting that LHP1 has functions similar to those of Polycomb PMID: 17676062
disruption of the chromodomain of LHP1 abolishes H3K27me3 recognition, releases gene silencing and causes similar phenotypic alterations as transcriptional lhp1 null mutants PMID: 19399177

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Subcellular Location

Nucleus. Note=Punctuated distribution and in heterochromatic chromocenters.

Tissue Specificity

Mainly expressed in meristematic tissues of vegetative, inflorescence and floral organs, like in developing ovules, petals, root pericycle, petiole and petiole side of developing leaf blade or vascular tissue.

Database Links

KEGG: ath:AT5G17690

STRING: 3702.AT5G17690.1

UniGene: At.7322

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LHP1 Antibody

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