Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans mdt-15 Polyclonal antibody

Uniprot No.

Q21955

Target Names

mdt-15

Alternative Names

mdt-15 antibody; R12B2.5Mediator of RNA polymerase II transcription subunit 15 antibody; Mediator complex subunit 15 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans mdt-15 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for regulated expression of genes controlling fatty acid desaturation by factors including sbp-1 and nhr-49.

Gene References into Functions

Prolongation of survival under glucose exposure by quercetin is dependent on SIR-2.1, DAF-12, and MDT-15 PMID: 24407905
MDT-15/MED15 is required for the xenobiotic-induced expression of p38 MAP kinase PMK-1-dependent immune genes and protection from Pseudomonas aeruginosa infection PMID: 24875643
These findings reveal novel conserved players in the oxidative stress response and suggest a broad cytoprotective role for MDT-15. PMID: 23957350
Mdt-15 worms exhibit disturbed endoplasmic reticulum (ER) homeostasis, as indicated by a constitutively activated endoplasmic reticulum unfolded protein response. PMID: 24843123
Certain compounds have increased toxicity when combined with a dietary restriction regimen through increased metabolic activation through the MDT-15 transcription factor. PMID: 22132200
A single subunit of the Mediator complex, MDT-15, integrates the activities of several distinct regulatory factors to coordinate metabolic and hormonal regulation of FA metabolism. PMID: 16651656
the C. elegans ARC105 homologue MDT-15 is required for fatty acid homeostasis, and both SBP-1 and MDT-15 control transcription of genes governing desaturation of stearic acid to oleic acid PMID: 16799563
propose a model whereby MDT-15 integrates several transcriptional regulatory pathways to monitor both the availability and quality of ingested materials, including nutrients and xenobiotic compounds PMID: 18454197

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Subcellular Location

Nucleus.

Protein Families

Mediator complex subunit 15 family

Tissue Specificity

Expressed in the intestine and head neurons.

Database Links

KEGG: cel:CELE_R12B2.5

STRING: 6239.R12B2.5a.1

UniGene: Cel.38778

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mdt-15 Antibody

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