Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MUS81 Polyclonal antibody

Uniprot No.

Q5W9E7

Target Names

MUS81

Alternative Names

MUS81 antibody; At4g30870 antibody; F6I18.220Crossover junction endonuclease MUS81 antibody; EC 3.1.22.- antibody; Protein MMS AND UV SENSITIVE 81 antibody; AtMUS81 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana MUS81 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks, nicked Holliday junctions and also intact Holliday junctions with a reduced efficiency. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. Plays a role in DNA repair and in genotoxic stress-induced homologous recombination (HR) in somatic cells. Mediates a subset of meiotic recombination events that are insensitive to crossover interference. Together with SEND1, essential for the resolution of toxic replication structures to ensure genome stability, and to maintain telomere integrity and replication.

Gene References into Functions

Data show that DNA polymerase zeta catalytic subunit REV3 cooperates with ATP-dependent DNA helicase RECQ4A and e ndonuclease MUS81 to repair DNA interstrand cross links. PMID: 26474640
RECQ4A and MUS81 define RAD5A-independent pathways of DNA repair. RECQ4A and MUS81 act in parallel pathways presumably to resolve replication-associated DNA intermediates. PMID: 20971895
The role of AtMUS81 in meiotic and mitotic recombination. PMID: 16945961
AtMUS81 is involved in a secondary subset of meiotic crossovers that are interference insensitive. PMID: 17696612
The role of MUS81 in the reproduction and growth of A. thaliana, via its expression and metabolism during meiosis, is reported. PMID: 18182028
Both MUS81-EME1 endonuclease complexes are involved in DNA recombination and repair processes in Arabidopsis. PMID: 19339504

Hide All

Subcellular Location

Nucleus. Nucleus, nucleolus.

Protein Families

XPF family

Tissue Specificity

Ubiquitous but preferentially expressed in young flowers buds, notably in anthers.

Database Links

KEGG: ath:AT4G30870

STRING: 3702.AT4G30870.1

UniGene: At.51242

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA149623XA01DOA
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

MUS81 Antibody

Product Details

Customer Reviews and Q&A

Target Background