Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NCED3 Polyclonal antibody

Uniprot No.

Q9LRR7

Target Names

NCED3

Alternative Names

NCED3 antibody; STO1 antibody; At3g14440 antibody; MOA2.4 antibody; 9-cis-epoxycarotenoid dioxygenase NCED3 antibody; chloroplastic antibody; AtNCED3 antibody; EC 1.13.11.51 antibody; Protein SALT TOLERANT 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana NCED3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids, in response to water stress.

Gene References into Functions

In wild-type leaves, methyl jasmonate (MeJA)stimulates the expression of NCED3, which encodes an enzyme in abscisic acid (ABA) biosynthesis. Endogenous ABA could be involved in MeJA signal transduction and lead to stomatal closure in the guard cells. PMID: 21402795
Overexpression of AtSTO1 improved salt tolerance in transgenic poplar. PMID: 24929937
A distal ABA responsive element in AtNCED3 promoter is required for positive feedback regulation of ABA biosynthesis in Arabidopsis. PMID: 24475264
An overlapping G-box recognition sequence (50-CACGTG-30) at 22248 base pairs from the transcriptional start site of AtNCED3 is an important cis-acting element in the induction of the dehydration response. PMID: 23604098
The ced1 (for 9-cis epoxycarotenoid dioxygenase defective 1) mutant isolated in this study showed markedly reduced expression of NCED3 in response to osmotic stress (polyethylene glycol) treatments compared with the wild type. PMID: 21610183
NCED5 thus contributes, together with NCED3, to abscisic acid production affecting plant growth and water stress tolerance. PMID: 22171989
ATX1 (histone-lysine N-methyltransferase) directly binds NCED3 and regulates its transcription. PMID: 21309869
Studies indicate that NCED3 encodes 9-cis-epoxycarotenoid dioxygenase that catalyzes the oxidative cleavage of a 9-cis isomer of epoxycarotenoid (9-cis-violaxanthin or 9'-cis-neoxanthin) to form xanthoxin. PMID: 21103336
The spatial constraint of AtNCED3 expression in vascular tissues provides a novel insight into plant systemic response to drought stresses. PMID: 18550687
Mutations in the SIS7/NCED3/STO1 gene confer a sugar-insensitive phenotype, which indicates that a role in ABA biosynthesis is not necessary to confer sugar insensitivity. PMID: 18854047
The 274th site (P<-->S) and the 327th site (P<-->R) amino acid variations might be the cause of abscisic acid content increase of 163av accession under drought stress. PMID: 19200156

Hide All

Subcellular Location

Plastid, chloroplast stroma. Note=Partially bound to the thylakoid.

Protein Families

Carotenoid oxygenase family

Tissue Specificity

Localized in roots, leaves, stems, empty silique envelopes and seeds. Expressed at the point of organ attachment and the abscission zones in the plant.

Database Links

KEGG: ath:AT3G14440

STRING: 3702.AT3G14440.1

UniGene: At.26346

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NCED3 Antibody

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